Antibody:	P469 antibody
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb mitral cell layer	Strong		7a
TS28: accessory olfactory bulb	Weak		7a

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Very strong		7b
TS28: taenia tecta	Moderate	Regionally restricted	7b	Expression is in the dorsal taenia tecta.
TS28: corpus striatum	Strong		7b
TS28: caudate-putamen	Strong	Regionally restricted	7b	There is moderate labeling of some of the striosomes.
TS28: bed nucleus of stria terminalis	Absent		7b

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Very strong		7c
TS28: arcuate nucleus	Strong		7c
TS28: caudate-putamen	Strong		7c
TS28: cerebral cortex	Strong		7c
TS28: corpus striatum	Strong		7c
TS28: indusium griseum	Strong		7c
TS28: septal nucleus	Strong	Regionally restricted	7c	Expression is in the triangular nucleus of the septum.
TS28: thalamus	Strong	Regionally restricted	7c	Most thalamic areas, including cell bodies and neuropil, contained moderate to strong expression.
TS28: hypothalamus	Moderate	Regionally restricted	7c
TS28: bed nucleus of stria terminalis	Absent		7c
TS28: cerebral internal capsule	Absent		7c
TS28: fimbria hippocampus	Absent		7c
TS28: fornix	Absent		7c
TS28: optic chiasma	Absent		7c

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Very strong		7d
TS28: caudate-putamen	Strong		7d
TS28: cerebral cortex	Strong		7d
TS28: corpus striatum	Strong		7d
TS28: lateral globus pallidus	Strong		7d
TS28: lateral septal nucleus	Strong		7d
TS28: medial geniculate nucleus	Strong		7d
TS28: ventral posterior lateral thalamic nucleus	Strong		7d
TS28: ventral posterior medial thalamic nucleus	Strong		7d
TS28: pallidum	Strong	Regionally restricted	7d	Expression is ventral.
TS28: thalamus	Strong	Regionally restricted	7d	Most thalamic areas, including cell bodies and neuropil, contained moderate to strong expression.
TS28: thalamic nucleus	Strong	Regionally restricted	7d	Expression is in the submedius thalamic nucleus.
TS28: anterior ventral thalamic nucleus	Weak		7d
TS28: bed nucleus of stria terminalis	Absent		7d
TS28: cerebral internal capsule	Absent		7d
TS28: optic chiasma	Absent		7d

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Very strong		7e
TS28: medial geniculate nucleus	Strong		7e
TS28: ventral posterior lateral thalamic nucleus	Strong		7e
TS28: ventral posterior medial thalamic nucleus	Strong		7e
TS28: thalamic nucleus	Strong	Regionally restricted	7e	Expression is in the submedius thalamic nucleus.

Structure	Level	Pattern	Image	Note
TS28: pontine nucleus	Very strong		7f
TS28: presubiculum	Very strong		7f
TS28: midbrain tegmentum	Strong		7f
TS28: red nucleus	Strong		7f
TS28: substantia nigra pars compacta	Present		7f
TS28: ventral tegmental area	Present		7f

Structure	Level	Pattern	Image	Note
TS28: pontine nucleus	Very strong		7g

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Very strong		7h
TS28: pontine nucleus	Very strong		7h
TS28: medulla oblongata	Very strong		7h	Expression is in neuropil and fibers.
TS28: cerebellum	Strong		7h
TS28: cerebellum white matter	Moderate		7h	There was low to moderate labeleing.
TS28: cerebellum Purkinje cell layer	Weak		7h
TS28: cerebellum molecular layer	Weak		7h

Structure	Level	Pattern	Image	Note
TS28: accumbens nucleus	Very strong		7i
TS28: cerebral cortex	Very strong		7i
TS28: dentate gyrus granule cell layer	Very strong		7i
TS28: olfactory tubercle	Very strong		7i
TS28: pontine nucleus	Very strong		7i
TS28: main olfactory bulb mitral cell layer	Strong		7i
TS28: medial geniculate nucleus	Strong		7i
TS28: ventral posterior lateral thalamic nucleus	Strong		7i
TS28: ventral posterior medial thalamic nucleus	Strong		7i
TS28: thalamus	Strong	Regionally restricted	7i	Most thalamic areas, including cell bodies and neuropil, contained moderate to strong expression.
TS28: thalamic nucleus	Strong	Regionally restricted	7i	Expression is in the submedius thalamic nucleus.
TS28: anterior commissure	Present		7i
TS28: corpus callosum	Weak		7i
TS28: main olfactory bulb external plexiform layer	Weak		7i

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb granule cell layer	Present		8a
TS28: main olfactory bulb mitral cell layer	Present		8a
TS28: main olfactory bulb external plexiform layer	Weak		8a

Structure	Level	Pattern	Image	Note
TS28: brain	Present		8b	Expression is in large and small neurons with their cell bodies and processes, as well as surrounding neuropil. Absent in glia or astroglial-like cells.

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Very strong		8c

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Very strong		8d
TS28: hippocampus CA1	Strong		8d
TS28: hippocampus CA2	Strong		8d
TS28: hippocampus CA3	Strong		8d

Structure	Level	Pattern	Image	Note
TS28: caudate-putamen	Strong	Regionally restricted	8i , 8g , 8h , 8e	There is moderate There is moderate labeling of some of the striosomes.labeling of some of the striosomes.
TS28: corpus striatum	Strong	Regionally restricted	8i , 8g , 8h , 8e	There is moderate There is moderate labeling of some of the striosomes.labeling of some of the striosomes.

Structure	Level	Pattern	Image	Note
TS28: arcuate nucleus	Strong		8f

Structure	Level	Pattern	Image	Note
TS28: pontine nucleus	Very strong		9a	Expression is in the cell bodies and fibers.

Structure	Level	Pattern	Image	Note
TS28: pontine nucleus	Very strong		9b	Expression is in the cell bodies and fibers.

Structure	Level	Pattern	Image	Note
TS28: pontine nucleus	Very strong		9c	Expression is in the cell bodies and fibers.

Structure	Level	Pattern	Image	Note
TS28: pontine nucleus	Very strong		9d	Expression is in the cell bodies and fibers.

Structure	Level	Pattern	Image	Note
TS28: area postrema	Present		9e
TS28: dorsal motor nucleus of vagus nerve	Present		9e
TS28: hypoglossal nucleus	Present		9e
TS28: brainstem	Present	Regionally restricted	9e	Expression is in the medial longitudinal fasciculus.`

Structure	Level	Pattern	Image	Note
TS28: medulla oblongata	Present		9g , 9f	Expression is in neuropil and fibers.
TS28: raphe obscurus nucleus	Present		9g , 9f	Expression is in neuropil and fibers.
TS28: raphe pallidus nucleus	Present		9g , 9f	Expression is in neuropil and fibers.

Structure	Level	Pattern	Image	Note
TS28: medulla oblongata	Very strong		9h	Expression is in neuropil and fibers.
TS28: trigeminal nerve	Weak	Regionally restricted	9h	Expression is in the spinal tract of the trigeminal nerve.

Structure	Level	Pattern	Image	Note
TS28: medulla oblongata	Very strong		9i	Expression is in neuropil and fibers.
TS28: gigantocellular reticular nucleus	Very strong		9i	Expression is in the neuropil and cell bodies.
TS28: inferior olivary nucleus	Very strong		9i	Expression is in the neuropil and cell bodies.
TS28: lateral reticular nucleus	Very strong		9i	Expression is in the neuropil and cell bodies.

Structure	Level	Pattern	Image	Note
TS28: medulla oblongata	Very strong		9k , 9j	Expression is in neuropil and fibers. Expression is weaker in the pyramid of the medulla.
TS28: gigantocellular reticular nucleus	Very strong		9j , 9k	Expression is in the neuropil and cell bodies.
TS28: inferior olivary nucleus	Very strong		9j , 9k	Expression is in the neuropil and cell bodies.
TS28: lateral reticular nucleus	Very strong		9j , 9k	Expression is in the neuropil and cell bodies.