Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Igs2tm2(Smarcd3-cre/ERT2)Bbr
Name:	intergenic site 2; targeted mutation 2, Benoit G Bruneau
MGI ID:	MGI:6790573
Synonyms:	F6CreERT2, Smarcd3-F6CreERT2
Gene:	Igs2  Location: unknown  Genetic Position: Chr11, Syntenic
Alliance:	Igs2tm2(Smarcd3-cre/ERT2)Bbr page

Germline Transmission:	Earliest citation of germline transmission: J:232545
Parent Cell Line:	Not Specified (ES Cell)
Strain of Origin:	Not Specified

Allele Type:    Targeted (Inducible, Recombinase) Inducer:    tamoxifen Mutation:    Insertion   Igs2tm2(Smarcd3-cre/ERT2)Bbr expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Smarcd3 (MGI:1914243)   Mutation details: Site-specific PhiC31 integrase-mediated transgenesis is used to insert H19 insulator flanked fragment of the Smarcd3-F6 fragment, including approximately 2.5 kb of the 5 end of the Smarcd3-F1 enhancer and Hsp68 minimal promoter followed by cre/ERT coding sequence and EF1 poly signal into the intergenic site 2 locus (Hipp11). This construct was microinjected, along with the PhiC31 integrase mRNA, into the pronuclei of zygotes collected from the TARGATT mice. (J:232545)

Activity:	Tissue activity of this recombinase allele
Driver:	Smarcd3 (mouse) Summary of all recombinase alleles driven by Smarcd3. Comparative matrix view of recombinase activities Alliance mouse Smarcd3 gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Igs2 Mutation:	68 strains or lines available

Original:	J:232545 Devine WP, et al., Early patterning and specification of cardiac progenitors in gastrulating mesoderm. Elife. 2014;3
All:	1 reference(s)