Slc29a4 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:122059 Dahlin A, et al., Expression and immunolocalization of the plasma membrane monoamine transporter in the brain. Neuroscience. 2007 May 25;146(3):1193-211
Assay type:	RNA in situ
MGI Accession ID:	MGI:6790478
Gene symbol:	Slc29a4
Gene name:	solute carrier family 29 (nucleoside transporters), member 4

Probe:	PMAT cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen 3a: postnatal adult (more

)

Structure	Level	Image
TS28: cerebral cortex	Very strong	3a
TS28: dentate gyrus	Very strong	3a
TS28: frontal association cortex	Very strong	3a
TS28: hippocampus CA1	Very strong	3a
TS28: hippocampus CA2	Very strong	3a
TS28: hippocampus CA3	Very strong	3a
TS28: main olfactory bulb	Very strong	3a
TS28: olfactory tubercle	Very strong	3a
TS28: nerve fiber tract	Absent	3a

Specimen 3b: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Very strong		3b
TS28: dentate gyrus	Very strong		3b
TS28: hippocampus CA1	Very strong		3b
TS28: hippocampus CA2	Very strong		3b
TS28: hippocampus CA3	Very strong		3b
TS28: main olfactory bulb	Very strong		3b
TS28: olfactory tubercle	Very strong		3b
TS28: prefrontal cortex	Strong	Regionally restricted	3b	Expression is in the motor cortex.
TS28: cingulate cortex	Strong		3b
TS28: prelimbic area	Strong		3b
TS28: visual cortex	Strong		3b
TS28: orbital area	Weak	Regionally restricted	3b	Expression is in the lateral orbital cortex.
TS28: taenia tecta	Present	Regionally restricted	3b	Expression is in the dorsal and ventral taenia tecta.
TS28: nerve fiber tract	Absent		3b

Specimen 3c: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Very strong		3c
TS28: dentate gyrus	Very strong		3c
TS28: hippocampus CA1	Very strong		3c
TS28: hippocampus CA2	Very strong		3c
TS28: hippocampus CA3	Very strong		3c
TS28: main olfactory bulb	Very strong		3c
TS28: olfactory tubercle	Very strong		3c
TS28: prefrontal cortex	Strong	Regionally restricted	3c	Expression is in the motor cortex.
TS28: 3rd ventricle choroid plexus epitheilum	Strong		3c
TS28: 4th ventricle choroid plexus epithelium	Strong		3c
TS28: cingulate cortex	Strong		3c
TS28: lateral ventricle choroid plexus epithelium	Strong		3c
TS28: medial preoptic area	Strong		3c
TS28: paraventricular thalamic nucleus	Strong		3c
TS28: prelimbic area	Strong		3c
TS28: somatosensory cortex	Strong		3c
TS28: subfornical organ	Strong		3c
TS28: visual cortex	Strong		3c
TS28: cortical amygdaloid nucleus	Moderate		3c
TS28: caudate-putamen	Moderate		3c	Moderate to dense labeling in many neurons.
TS28: corpus striatum	Moderate		3c	Moderate to dense labeling in many neurons.
TS28: bed nucleus of stria terminalis	Strong	Regionally restricted	3c	Expression is in the supracapsular part.
TS28: cerebral internal capsule	Weak		3c
TS28: globus pallidus	Weak		3c
TS28: nerve fiber tract	Absent		3c

Specimen 3d: postnatal adult (more

)

Structure	Level	Image
TS28: cerebral cortex	Very strong	3d
TS28: dentate gyrus	Very strong	3d
TS28: dentate gyrus granule cell layer	Very strong	3d
TS28: hippocampus CA1	Very strong	3d
TS28: hippocampus CA2	Very strong	3d
TS28: hippocampus CA3	Very strong	3d
TS28: main olfactory bulb	Very strong	3d
TS28: olfactory tubercle	Very strong	3d
TS28: cortical amygdaloid nucleus	Moderate	3d
TS28: midbrain	Weak	3d
TS28: nerve fiber tract	Absent	3d

Specimen 3e: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Very strong		3e
TS28: cerebral cortex	Very strong		3e
TS28: dentate gyrus	Very strong		3e
TS28: hippocampus CA1	Very strong		3e
TS28: hippocampus CA2	Very strong		3e
TS28: hippocampus CA3	Very strong		3e
TS28: main olfactory bulb	Very strong		3e
TS28: olfactory tubercle	Very strong		3e
TS28: 3rd ventricle choroid plexus epitheilum	Strong		3e
TS28: 4th ventricle choroid plexus epithelium	Strong		3e
TS28: lateral ventricle choroid plexus epithelium	Strong		3e
TS28: medulla oblongata	Moderate	Regionally restricted	3e	Overall expression was light to moderate with scattered cells with intense staining.
TS28: nerve fiber tract	Absent		3e

Specimen 3f: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Very strong		3f
TS28: cerebral cortex	Very strong		3f
TS28: dentate gyrus	Very strong		3f
TS28: dentate gyrus granule cell layer	Very strong		3f
TS28: hippocampus CA1	Very strong		3f
TS28: hippocampus CA2	Very strong		3f
TS28: hippocampus CA3	Very strong		3f
TS28: main olfactory bulb	Very strong		3f
TS28: olfactory tubercle	Very strong		3f
TS28: arcuate nucleus	Strong		3f
TS28: red nucleus	Strong		3f
TS28: substantia nigra	Strong		3f
TS28: ventral tegmental area	Strong		3f
TS28: medulla oblongata	Moderate	Regionally restricted	3f	Overall expression was light to moderate with scattered cells with intense staining.
TS28: midbrain	Weak		3f
TS28: nerve fiber tract	Absent		3f

Specimen 4a: postnatal adult (more

)

Structure	Level	Image
TS28: main olfactory bulb glomerular layer	Very strong	4a
TS28: main olfactory bulb mitral cell layer	Very strong	4a
TS28: main olfactory bulb	Strong	4a

Specimen 4b: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Strong		4b
TS28: cortical layer II	Strong		4b

Specimen 4c: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Strong		4c
TS28: cerebral cortex	Present		4c

Specimen 4d: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: piriform cortex	Very strong		4d

Specimen 4e: postnatal adult (more

)

Structure	Level	Image
TS28: dentate gyrus granule cell layer	Very strong	4e
TS28: hippocampus CA1	Strong	4e
TS28: hippocampus CA2	Strong	4e
TS28: hippocampus CA3	Strong	4e

Specimen 4g: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Very strong		4f , 4g	Cytoplasmic staining.

Specimen 4h: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: caudate-putamen	Moderate		4h	Moderate to dense labeling in many neurons.
TS28: corpus striatum	Moderate		4h	Moderate to dense labeling in many neurons.

Specimen 4i: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: arcuate nucleus	Strong		4i

Specimen 4j: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: arcuate nucleus	Strong		4j

Specimen 5a: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: superior olivary nucleus	Very strong		5a
TS28: hypothalamus	Strong		5a
TS28: peduncular pontine nucleus	Strong		5a
TS28: pontine nucleus	Strong		5a
TS28: red nucleus	Strong		5b , 5a , 5d
TS28: substantia nigra	Strong		5b , 5a , 5d
TS28: ventral tegmental area	Strong		5b , 5a , 5d
TS28: brain nucleus	Strong	Regionally restricted	5a	Expression is in the interstitial nucleus of the medial longitudinal fasciculus.

Specimen 5c: postnatal adult (more

)

Structure	Level	Image	Note
TS28: substantia nigra pars compacta	Strong	5c
TS28: ventral tegmental area	Strong	5c
TS28: substantia nigra	Strong	5c	There is dense cytoplasmic labeling.
TS28: substantia nigra pars reticulata	Absent	5c

Specimen 5e: postnatal adult (more

)

Structure	Level	Image
TS28: medial geniculate nucleus	Strong	5e
TS28: hippocampus CA3	Present	5e
TS28: nerve fiber tract	Absent	5e

Specimen 5f: postnatal adult (more

)

Structure	Level	Image
TS28: cerebellum Purkinje cell layer	Very strong	5f
TS28: cerebellum granule cell layer	Very strong	5f
TS28: cerebellum molecular layer	Moderate	5f
TS28: cerebellum white matter	Absent	5f
TS28: nerve fiber tract	Absent	5f

Specimen 5g: postnatal adult (more

)

Structure	Level	Image
TS28: 3rd ventricle choroid plexus epitheilum	Very strong	5g
TS28: 4th ventricle choroid plexus epithelium	Very strong	5g
TS28: lateral ventricle choroid plexus epithelium	Very strong	5g
TS28: brain	Present	5g

Specimen not shown: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: perirhinal cortex	Present
TS28: temporal lobe	Present

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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