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Gene Expression Data
Assay Details
Assay
Reference: J:312000 Bell BJ, et al., A clock-driven neural network critical for arousal. bioRxiv. 2020;
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6784071
Gene symbol: Ankfn1
Gene name: ankyrin-repeat and fibronectin type III domain containing 1
Reporter: tdTomato
Probe: tdTomato probe (317041)
Probe preparation: Antisense
Visualized with: Fluorescence
Assay notes: RNAscope assay.
Results
Specimen ExtData 6d: postnatal month 2-4; Ankfn1tm2.1(cre)Mnw/Ankfn1+ (more )
Note: Double labeled: red - Ankfn1 reporter; green - Slc17a6 (assay MGI:6784072). Also stained with DAPI (blue).
Structure Level Pattern Image Note
TS28: dorsomedial hypothalamic nucleus Present Extended Data 6d
Specimen ExtData 6e: postnatal month 2-4; Ankfn1tm2.1(cre)Mnw/Ankfn1+ (more )
Note: Double labeled: red - Ankfn1 reporter; green - Slc17a6 (assay MGI:6784072). Also stained with DAPI (blue).
Structure Level Pattern Image Note
TS28: dorsomedial hypothalamic nucleus Present Extended Data 6e
Specimen ExtData 6f: postnatal month 2-4; Ankfn1tm2.1(cre)Mnw/Ankfn1+ (more )
Note: Double labeled: red - Ankfn1 reporter; white - Slc32a1 (assay MGI:6784073). Also stained with DAPI (blue).
Structure Level Pattern Image Note
TS28: suprachiasmatic nucleus Present Extended Data 6f
Specimen ExtData 6g: postnatal month 2-4; Ankfn1tm2.1(cre)Mnw/Ankfn1+ (more )
Note: Double labeled: red - Ankfn1 reporter; white - Slc32a1 (assay MGI:6784073). Also stained with DAPI (blue).
Structure Level Pattern Image Note
TS28: suprachiasmatic nucleus Present Extended Data 6g
Specimen ExtData 8f: postnatal month 2-4; Ankfn1tm2.1(cre)Mnw/Ankfn1+ (more )
Note: Double labeled: red - Ankfn1 reporter; green - Slc17a6 (assay MGI:6784072). Also stained with DAPI (blue).
Structure Level Pattern Image Note
TS28: locus coeruleus Present Extended Data 8f
Specimen ExtData 8g: postnatal month 2-4; Ankfn1tm2.1(cre)Mnw/Ankfn1+ (more )
Note: Double labeled: red - Ankfn1 reporter; white - Slc32a1 (assay MGI:6784073). Also stained with DAPI (blue).
Structure Level Pattern Image Note
TS28: locus coeruleus Present Extended Data 8g
Specimen ExtData 8h: postnatal month 2-4; Ankfn1tm2.1(cre)Mnw/Ankfn1+ (more )
Note: Double labeled: red - Ankfn1 reporter; green - Slc17a6 (assay MGI:6784072). Also stained with DAPI (blue).
Structure Level Pattern Image Note
TS28: basal forebrain Present Extended Data 8h
Specimen ExtData 8i: postnatal month 2-4; Ankfn1tm2.1(cre)Mnw/Ankfn1+ (more )
Note: Double labeled: red - Ankfn1 reporter; white - Slc32a1 (assay MGI:6784073). Also stained with DAPI (blue).
Structure Level Pattern Image Note
TS28: basal forebrain Present Extended Data 8i
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory