Gene Expression Data
Assay Details
Assay
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Reference:
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J:312000
Bell BJ, et al., A clock-driven neural network critical for arousal. bioRxiv. 2020;
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6784073
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Gene symbol:
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Slc32a1
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Gene name:
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solute carrier family 32 (GABA vesicular transporter), member 1
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Probe:
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Mm-Slc32a1 (319191)
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Probe preparation:
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Antisense
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Visualized with:
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Fluorescence
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Assay notes:
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RNAscope assay.
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Results
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Specimen
ExtData 6f:
postnatal month 2-4; Ankfn1tm2.1(cre)Mnw/Ankfn1 +
(more )
Note: Double labeled: white - Slc32a1; red - Ankfn1 reporter (assay MGI:6784071). Also stained with DAPI (blue).
Specimen
ExtData 6f:
(close )
Genetic Background:
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involves: 129S/SvEv * C57BL/6
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Mutant Allele(s):
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Ankfn1tm2.1(cre)Mnw/Ankfn1+
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Age:
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postnatal month 2-4
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Sex:
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Male
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Double labeled: white - Slc32a1; red - Ankfn1 reporter (assay MGI:6784071). Also stained with DAPI (blue).
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Structure |
Level |
Pattern |
Image |
Note |
TS28: suprachiasmatic nucleus |
Present |
Regionally restricted |
Extended Data 6f
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Expression is seen in GABAergic neurons.
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Specimen
ExtData 6g:
postnatal month 2-4; Ankfn1tm2.1(cre)Mnw/Ankfn1 +
(more )
Note: Double labeled: white - Slc32a1; red - Ankfn1 reporter (assay MGI:6784071). Also stained with DAPI (blue).
Specimen
ExtData 6g:
(close )
Genetic Background:
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involves: 129S/SvEv * C57BL/6
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Mutant Allele(s):
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Ankfn1tm2.1(cre)Mnw/Ankfn1+
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Age:
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postnatal month 2-4
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Sex:
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Male
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
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Double labeled: white - Slc32a1; red - Ankfn1 reporter (assay MGI:6784071). Also stained with DAPI (blue).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: suprachiasmatic nucleus |
Present |
Regionally restricted |
Extended Data 6g
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Expression is seen in GABAergic neurons.
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Specimen
ExtData 8g:
postnatal month 2-4; Ankfn1tm2.1(cre)Mnw/Ankfn1 +
(more )
Note: Double labeled: white - Slc32a1; red - Ankfn1 reporter (assay MGI:6784071). Also stained with DAPI (blue).
Specimen
ExtData 8g:
(close )
Genetic Background:
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involves: 129S/SvEv * C57BL/6
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Mutant Allele(s):
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Ankfn1tm2.1(cre)Mnw/Ankfn1+
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Age:
|
postnatal month 2-4
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Sex:
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Male
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Note:
|
Double labeled: white - Slc32a1; red - Ankfn1 reporter (assay MGI:6784071). Also stained with DAPI (blue).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: brain |
Present |
Regionally restricted |
Extended Data 8g
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Expression is seen in GABAergic neurons.
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Specimen
ExtData 8i:
postnatal month 2-4; Ankfn1tm2.1(cre)Mnw/Ankfn1 +
(more )
Note: Double labeled: white - Slc32a1; red - Ankfn1 reporter (assay MGI:6784071). Also stained with DAPI (blue).
Specimen
ExtData 8i:
(close )
Genetic Background:
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involves: 129S/SvEv * C57BL/6
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Mutant Allele(s):
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Ankfn1tm2.1(cre)Mnw/Ankfn1+
|
Age:
|
postnatal month 2-4
|
Sex:
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Male
|
Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
|
Cryosection
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Note:
|
Double labeled: white - Slc32a1; red - Ankfn1 reporter (assay MGI:6784071). Also stained with DAPI (blue).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: brain |
Present |
Regionally restricted |
Extended Data 8i
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Expression is seen in GABAergic neurons.
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