Gene Expression Data
Assay Details
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Assay
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Reference:
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J:306181
Crosse EI, et al., Multi-layered Spatial Transcriptomics Identify Secretory Factors Promoting Human Hematopoietic Stem Cell Development. Cell Stem Cell. 2020 Nov 5;27(5):822-839.e8
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6780093
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Gene symbol:
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Edn1
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Gene name:
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endothelin 1
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Antibody:
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anti-End1 (ab117757)
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Assay notes:
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The authors reported using two antibodies but did not specify which was used to obtain these data; it is possible MGI:6780083 was used.
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Results
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Specimen
7A':
embryonic day 10.5
(more  )
Note: Triple labeled: yellow - Edn1; teal - Kit (assay MGI:6780092); purple - Pecam1 (assay MGI:6780091).
Specimen
7A':
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: yellow - Edn1; teal - Kit (assay MGI:6780092); purple - Pecam1 (assay MGI:6780091).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: dorsal aorta |
Present |
Regionally restricted |
7A'' right
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7A'' left
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7A'
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Expression is seen in emerging intra-aortic hematopoietic cell clusters, mainly on cell membranes exposed to the aortic lumen and excluded from cell-cell contact interfaces. Weaker staining is also observed throughout the subaortic mesenchyme.
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Analysis Tools
