Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:306181
Crosse EI, et al., Multi-layered Spatial Transcriptomics Identify Secretory Factors Promoting Human Hematopoietic Stem Cell Development. Cell Stem Cell. 2020 Nov 5;27(5):822-839.e8
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:6780091
|
|
Gene symbol:
|
Pecam1
|
|
Gene name:
|
platelet/endothelial cell adhesion molecule 1
|
|
Antibody:
|
MEC 13.3
|
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor
|
|
Assay notes:
|
The authors reported using two antibodies but did not specify which was used to obtain these data; it is possible MGI:6780085 was used.
|
|
|
Results
|
Specimen
7A':
embryonic day 10.5
(more  )
Note: Triple labeled: purple - Pecam1; teal - Kit (assay MGI:6780092); yellow - Edn1 (assay MGI:6780093).
Specimen
7A':
(close  )
|
Genetic Background:
|
C57BL/6J
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Triple labeled: purple - Pecam1; teal - Kit (assay MGI:6780092); yellow - Edn1 (assay MGI:6780093).
|
|
Analysis Tools
