Avpr1b RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:310526 Williams Avram SK, et al., NMDA Receptor in Vasopressin 1b Neurons Is Not Required for Short-Term Social Memory, Object Memory or Aggression. Front Behav Neurosci. 2019;13:218
Assay type:	RNA in situ
MGI Accession ID:	MGI:6765926
Gene symbol:	Avpr1b
Gene name:	arginine vasopressin receptor 1B

Probe:	Avpr1b probe (VB1-16867)
Probe preparation:	Antisense labelled with Alexa Fluor DNA
Visualized with:	Fluorescence
Assay notes:	ViewRNA assay.

Results

1C ©

1C inset ©

Specimen 1C: postnatal adult; Avpr1b^{tm2.1(cre)Wsy}/Avpr1b⁺ (more

)
Note: Dorsal hippocampus. Double labeled: green - Avpr1b message; pink - Avpr1b reporter (assay MGI:6764458).

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA2	Present		1C , 1C inset	Expression is seen in pyramidal cells.
TS28: hippocampus CA3	Present	Regionally restricted	1C , 1C inset	Expression is seen in areas immediately adjacent to CA2.
TS28: fasciola cinereum	Present		1C

1D ©

1D inset ©

Specimen 1D: postnatal adult (more

)
Note: Dorsal hippocampus. Wild type animal. Double labeled: green - Avpr1b; pink - Amigo2 (assay MGI:6765929).

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA2	Present		1D , 1D inset
TS28: fasciola cinereum	Present		1D

1E ©

1E inset ©

Specimen 1E: postnatal adult (more

)
Note: Dorsal hippocampus. Wild type animal. Double labeled: green - Avpr1b; pink - Trpc4 (assay MGI:6765930).

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA2	Present		1E inset , 1E	There is minimal cellular colocalization of Avpr1 with CA1 markers in the dorsal hippocampus.
TS28: fasciola cinereum	Present		1E

1F ©

1F inset ©

Specimen 1F: postnatal adult (more

)
Note: Dorsal hippocampus. Wild type animal. Double labeled: green - Avpr1b; pink - Bok (assay MGI:6765931).

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA2	Present		1F , 1F inset	There is minimal cellular colocalization of Avpr1 with CA3 markers in the dorsal hippocampus.
TS28: fasciola cinereum	Present		1F

1G ©

1G CA2 inset ©

Specimen 1G: postnatal adult (more

)
Note: Ventral hippocampus. Wild type animal. Double labeled: green - Avpr1b; pink - Amigo2 (assay MGI:6765929).

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA2	Present		1G , 1G CA2 inset

4A ©

4B ©

Specimen 4A/B: postnatal day 1; Avpr1b^{tm2.1(cre)Wsy}/Avpr1b⁺ (more

)
Note: Dorsal hippocampus.

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Weak		4B , 4A	Sparce staining is observed.

4C ©

4D ©

Specimen 4C/D: postnatal day 1; Avpr1b^{tm2.1(cre)Wsy}/Avpr1b⁺ (more

)
Note: Dorsal hippocampus.

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Weak		4C , 4D	Sparce staining is observed.

4E ©

4F ©

Specimen 4E/F: postnatal day 7; Avpr1b^{tm2.1(cre)Wsy}/Avpr1b⁺ (more

)
Note: Dorsal hippocampus.

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA2	Strong		4F , 4E	Robust staining is observed.

4G ©

4H ©

Specimen 4G/H: postnatal day 7; Avpr1b^{tm2.1(cre)Wsy}/Avpr1b⁺ (more

)
Note: Dorsal hippocampus.

Structure	Level	Pattern	Image	Note
TS28: fasciola cinereum	Strong		4H , 4G	Robust staining is observed.

5A upper ©

Specimen 5A CA2: postnatal adult (more

)
Note: Wild type animal.

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA2	Present		5A upper

5A lower ©

Specimen 5A FC: postnatal adult (more

)
Note: Wild type animal.

Structure	Level	Pattern	Image	Note
TS28: fasciola cinereum	Present		5A lower

5B upper ©

Specimen 5B CA2: postnatal adult; Avpr1b^{tm2.1(cre)Wsy}/Avpr1b⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA2	Present		5B upper	Expression levels are comparable to those seen in wild type.

5B lower ©

Specimen 5B FC: postnatal adult; Avpr1b^{tm2.1(cre)Wsy}/Avpr1b⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: fasciola cinereum	Present		5B lower	Expression levels are comparable to those seen in wild type.

5C upper ©

Specimen 5C CA2: postnatal adult; Avpr1b^{tm2.1(cre)Wsy}/Avpr1b^{tm2.1(cre)Wsy} (more

)

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA2	Absent		5C upper

5C lower ©

Specimen 5C FC: postnatal adult; Avpr1b^{tm2.1(cre)Wsy}/Avpr1b^{tm2.1(cre)Wsy} (more

)

Structure	Level	Pattern	Image	Note
TS28: fasciola cinereum	Absent		5C lower

6A ©

Specimen 6A: postnatal adult; Grin1^tm2Stl/? (more

)
Note: Dorsal hippocampus. Wild type phenotype; either fl/fl or fl/+. Double labeled: green - Avpr1b; red - Grin1 (assay MGI:6765933).

Structure	Level	Pattern	Image	Note
TS28: fasciola cinereum	Present		6A
TS28: hippocampus CA2	Present		6A

6B ©

6C ©

6D ©

Specimen 6B: postnatal adult; Avpr1b^{tm2.1(cre)Wsy}/Avpr1b⁺, Grin1^tm2Stl/Grin1^tm2Stl (more

)
Note: Conditional mutant. Dorsal hippocampus. Double labeled: green - Avpr1b; red - Grin1 (assay MGI:6765933).

Structure	Level	Pattern	Image	Note
TS28: fasciola cinereum	Present		6B , 6C
TS28: hippocampus CA2	Present		6B , 6D

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