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Gene Expression Data
Assay Details
Assay
Reference: J:265712 Dziedzic SA, et al., ABIN-1 regulates RIPK1 activation by linking Met1 ubiquitylation with Lys63 deubiquitylation in TNF-RSC. Nat Cell Biol. 2018 Jan;20(1):58-68
Assay type: Western blot
MGI Accession ID: MGI:6761348
Gene symbol: Mlkl
Gene name: mixed lineage kinase domain-like
Antibody: Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab196436)
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: Tissues were harvested in NP-40 lysis buffer, then treated with 6M urea to show the insoluble fraction, which is known to occur when cells undergo necroptosis. The antibody used recognizes the phosphorylated form of the protein.
Results Image: 7b lower p-MLKL
 Sample Information Bands Other Sample Information
Lane AgeStructure 50 kDa Genetic BackgroundMutant Allele(s)Sex
Abin+/+ E16.5 TS24: limb
TS24: tail
TS24: trunk 		Absent involves: C57BL/6J Not Specified
Abin-/- 1 E16.5 TS24: limb
TS24: tail
TS24: trunk 		Present involves: C57BL/6J Tnip1tm1.1Ama/Tnip1tm1.1AmaNot Specified
Abin-/- 2 E16.5 TS24: limb
TS24: tail
TS24: trunk 		Present involves: C57BL/6J Tnip1tm1.1Ama/Tnip1tm1.1AmaNot Specified
Abin-/- Ripk3-/- E16.5 TS24: limb
TS24: tail
TS24: trunk 		Absent involves: C57BL/6J * C57BL/6N Ripk3tm1Vmd/Ripk3tm1Vmd, Tnip1tm1.1Ama/Tnip1tm1.1AmaNot Specified
Abin-/- Ripk1D138N E16.5 TS24: limb
TS24: tail
TS24: trunk 		Absent involves: C57BL/6 * C57BL/6J Ripk1tm1.1Mkel/Ripk1tm1.1Mkel, Tnip1tm1.1Ama/Tnip1tm1.1AmaNot Specified
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory