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Gene Expression Data
Assay Details
Assay
Reference: J:265712 Dziedzic SA, et al., ABIN-1 regulates RIPK1 activation by linking Met1 ubiquitylation with Lys63 deubiquitylation in TNF-RSC. Nat Cell Biol. 2018 Jan;20(1):58-68
Assay type: Western blot
MGI Accession ID: MGI:6761345
Gene symbol: Ripk1
Gene name: receptor (TNFRSF)-interacting serine-threonine kinase 1
Antibody: RIP (D94C12) XP Rabbit mAb #3493
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: Tissues were harvested in NP-40 lysis buffer, then treated with 6M urea to show the insoluble fraction, which is known to occur when cells undergo necroptosis.
Results Image: 7b lower RIPK1
 Sample Information Bands Other Sample Information
Lane AgeStructure 75 kDa Genetic BackgroundMutant Allele(s)Sex
Abin+/+ E16.5 TS24: limb
TS24: tail
TS24: trunk 		Weak involves: C57BL/6J Not Specified
Abin-/- 1 E16.5 TS24: limb
TS24: tail
TS24: trunk 		Strong involves: C57BL/6J Tnip1tm1.1Ama/Tnip1tm1.1AmaNot Specified
Abin-/- 2 E16.5 TS24: limb
TS24: tail
TS24: trunk 		Strong involves: C57BL/6J Tnip1tm1.1Ama/Tnip1tm1.1AmaNot Specified
Abin-/- Ripk3-/- E16.5 TS24: limb
TS24: tail
TS24: trunk 		Weak involves: C57BL/6J * C57BL/6N Ripk3tm1Vmd/Ripk3tm1Vmd, Tnip1tm1.1Ama/Tnip1tm1.1AmaNot Specified
Abin-/- Ripk1D138N E16.5 TS24: limb
TS24: tail
TS24: trunk 		Trace involves: C57BL/6 * C57BL/6J Ripk1tm1.1Mkel/Ripk1tm1.1Mkel, Tnip1tm1.1Ama/Tnip1tm1.1AmaNot Specified
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
MGI 6.24 		The Jackson Laboratory