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Gene Expression Data
Assay Details
Assay
Reference: J:278916 Maruyama K, et al., Isl1-expressing non-venous cell lineage contributes to cardiac lymphatic vessel development. Dev Biol. 2019 Aug 15;452(2):134-143
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6758981
Gene symbol: Flt4
Gene name: FMS-like tyrosine kinase 4
Antibody: anti-VEGFR3 (AF743)
Detection system: Secondary antibody coupled to Alexa Fluor 555
Results
Specimen 5A/B: embryonic day 9.5 (more )
Note: Left lateral. Triple labeled: red - Flt4; green - Prox1 (assay MGI:6758996); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS15: 2nd branchial arch Present Regionally restricted 5A , 5B Prox1-VEGFR3 , 5B Merge , 5B VEGFR3 Flt4+/Pecam1+ cells were located mainly in the periphery of the Prox1+ cell cluster, with partial co-expression of Prox1.
TS15: 1st branchial arch Present Regionally restricted 5A Flt4+/Pecam1+ cells were located mainly in the periphery of the Prox1+ cell cluster, with partial co-expression of Prox1.
Specimen 5C/D: embryonic day 10.5 (more )
Note: Left lateral. Triple labeled: red - Flt4; green - Prox1 (assay MGI:6758996); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS17: 1st branchial arch Present Regionally restricted 5D Merge , 5D VEGFR3 , 5C , 5D Prox1-VEGFR3 Flt4+/Pecam1+ cells with and without Prox1 expression appeared to form mesh-like network structures, which extended caudally.
TS17: 2nd branchial arch Present Regionally restricted 5D Merge , 5D VEGFR3 , 5C , 5D Prox1-VEGFR3 Flt4+/Pecam1+ cells with and without Prox1 expression appeared to form mesh-like network structures, which extended caudally.
Specimen 5E/F: embryonic day 12.5 (more )
Note: Ventral side. Triple labeled: red - Flt4; green - Prox1 (assay MGI:6758996); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS20: lymphatic system Present Regionally restricted 5F Prox1-VEGFR3 , 5F VEGFR3 , 5F Merge , 5E Mesh-like networks composed of Prox1+ and/or Flt4+ cells appeared, surrounding the outflow tract.
Specimen 5G/H: embryonic day 15.0 (more )
Note: Ventral side. Triple labeled: red - Flt4; green - Prox1 (assay MGI:6758996); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS23: lymphatic vessel endothelium Present Regionally restricted 5G , 5H VEGFR3 , 5H Merge , 5H Prox1-VEGFR3 Pecam1+/Prox1+/Flt4+ thick cardiac lymphatics were recognized on the ventral side of the aorta.
Specimen 5I/J: embryonic day 16.5 (more )
Note: Ventral side. Triple labeled: red - Flt4; green - Prox1 (assay MGI:6758996); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS24: lymphatic vessel endothelium Present Regionally restricted 5J Merge , 5I , 5J Prox1-VEGFR3 , 5J VEGFR3 Pecam1+/Prox1+/Flt4+ cells around the outflow tract were remodeled and formed mature cardiac lymphatics.
Specimen 6I: embryonic day 15.0 (more )
Note: Dorsal side. Triple labeled: red - Flt4; green - Prox1 (assay MGI:6758996); blue - Pecam1 (assay MGI:6758967).
Structure Level Pattern Image Note
TS23: lymphatic vessel endothelium Present Regionally restricted 6I right , 6I left On the dorsal side of the hearts, Pecam1+/Prox1+/Flt4+ mature cardiac lymphatic vessels extended over the ventricular wall.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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