Arid1a Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:134633 Gao X, et al., ES cell pluripotency and germ-layer formation require the SWI/SNF chromatin remodeling component BAF250a. Proc Natl Acad Sci U S A. 2008 May 6;105(18):6656-61
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6757183
Gene symbol:	Arid1a
Gene name:	AT-rich interaction domain 1A

Antibody:	Anti-BAF250a
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

1B right ©

Specimen 1B: embryonic day 3.5 (more

)

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Very strong		1B right

Specimen S3A control: embryonic day 3.5 (more

)
Note: Double labeled: green - Arid1a; red - Pou5f1 (assay MGI:6757181).

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present		S3A Control merge , S3A Control BAF250a

Specimen S3A -/-: embryonic day 3.5; Arid1a^tm1.2Zhwa/Arid1a^tm1.2Zhwa (more

)
Note: Double labeled: green - Arid1a; red - Pou5f1 (assay MGI:6757181).

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Ambiguous		S3A -/- merge , S3A -/- BAF250a

Specimen S3B control: embryonic day 3.5 (more

)
Note: Double labeled: green - Arid1a; red - Cdx2 (assay MGI:6757191).

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present		S3B Control merge , S3B Control BAF250a

Specimen S3B -/-: embryonic day 3.5; Arid1a^tm1.2Zhwa/Arid1a^tm1.2Zhwa (more

)
Note: Double labeled: green - Arid1a; red - Cdx2 (assay MGI:6757191).

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Ambiguous		S3B -/- merge , S3B -/- BAF250a

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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