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Gene Expression Data
Assay Details
Assay
Reference: J:191065 Kang M, et al., FGF4 is required for lineage restriction and salt-and-pepper distribution of primitive endoderm factors but not their initial expression in the mouse. Development. 2013 Jan;140(2):267-79
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6756341
Gene symbol: Gata6
Gene name: GATA binding protein 6
Antibody: anti-GATA6
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1A': embryonic day 2.5 (more )
Note: 21 cells, late morula embryo. Double labeled: red - Gata6; white - Nanog (assay MGI:6756421).
Structure Level Pattern Image Note
TS4: inner cell mass Present 1A' , 1A Expression is in the nuclei of the majority of inner cell mass cells.
Specimen 1B': embryonic day 2.5; Fgf4tm1.3Mrt/Fgf4tm1.3Mrt (more )
Note: 21 cells, late morula embryo. The authors worked with two mutants that had identical phenotypes; it is possible this specimen carried Fgf4tm1Gol instead of Fgf4tm1.3Mrt. Double labeled: red - Gata6; white - Nanog (assay MGI:6756421).
Structure Level Pattern Image Note
TS4: inner cell mass Present 1B , 1B' Expression in this mutant is the same as in wild-type.
Specimen 1C': embryonic day 3.0 (more )
Note: 35 cells, early blastocyst. Double labeled: red - Gata6; white - Nanog (assay MGI:6756421).
Structure Level Pattern Image Note
TS4: inner cell mass Present 1C' , 1C Expression is in the nuclei of the majority of inner cell mass cells.
Specimen 1D': embryonic day 3.0; Fgf4tm1.3Mrt/Fgf4tm1.3Mrt (more )
Note: 35 cells, early blastocyst. The authors worked with two mutants that had identical phenotypes; it is possible this specimen carried Fgf4tm1Gol instead of Fgf4tm1.3Mrt. Double labeled: red - Gata6; white - Nanog (assay MGI:6756421).
Structure Level Pattern Image Note
TS4: inner cell mass Present 1D , 1D' Expression in this mutant is the same as in wild-type.
Specimen 1E': embryonic day 3.5 (more )
Note: 51 cells, early blastocyst. Double labeled: red - Gata6; white - Nanog (assay MGI:6756421).
Structure Level Pattern Image Note
TS5: inner cell mass Present Regionally restricted 1E' , 1E Expression in primitive endoderm progenitors.
Specimen 1F': embryonic day 3.5; Fgf4tm1.3Mrt/Fgf4tm1.3Mrt (more )
Note: 56 cells, early blastocyst. The authors worked with two mutants that had identical phenotypes; it is possible this specimen carried Fgf4tm1Gol instead of Fgf4tm1.3Mrt. Double labeled: red - Gata6; white - Nanog (assay MGI:6756421).
Structure Level Pattern Image Note
TS5: inner cell mass Absent 1F' , 1F
Specimen 1G': embryonic day 4.0 (more )
Note: 80 cells, mid blastocyst. Double labeled: red - Gata6; white - Nanog (assay MGI:6756421).
Structure Level Pattern Image Note
TS5: inner cell mass Present Regionally restricted 1G' , 1G Expression in primitive endoderm progenitors.
Specimen 1H': embryonic day 4.0; Fgf4tm1.3Mrt/Fgf4tm1.3Mrt (more )
Note: 84 cells, mid blastocyst. The authors worked with two mutants that had identical phenotypes; it is possible this specimen carried Fgf4tm1Gol instead of Fgf4tm1.3Mrt. Double labeled: red - Gata6; white - Nanog (assay MGI:6756421).
Structure Level Pattern Image Note
TS5: inner cell mass Absent 1H , 1H'
Specimen 1I': embryonic day 4.5 (more )
Note: >100cells, late blastocyst. Double labeled: red - Gata6; white - Nanog (assay MGI:6756421).
Structure Level Pattern Image Note
TS5: inner cell mass Present Regionally restricted 1I' , 1I Expression in primitive endoderm progenitors.
Specimen 1J': embryonic day 4.5; Fgf4tm1.3Mrt/Fgf4tm1.3Mrt (more )
Note: The authors worked with two mutants that had identical phenotypes; it is possible this specimen carried Fgf4tm1Gol instead of Fgf4tm1.3Mrt. Double labeled: red - Gata6; white - Nanog (assay MGI:6756421).
Structure Level Pattern Image Note
TS5: inner cell mass Absent 1J , 1J'
Specimen 2A zFgf4+/+: embryonic day 2.0 (more )
Note: 15 cell, morula stage embryo. Double labeled: red - Gata6; white - Nanog (assay MGI:6756421).
Structure Level Pattern Image Note
TS3: compacted morula Present 2A zFgf4+/+ Merge , 2A zFgf4+/+ GATA6
Specimen 2A mFgf4-/-; zFgf4+/-: embryonic day 2.0; Fgf4tm1.2Mrt/Fgf4+, Tg(Msx2-cre)5Rem/0 (more )
Note: Conditional mutant. 16 cells, morula stage. Double labeled: red - Gata6; white - Nanog (assay MGI:6756421).
Structure Level Pattern Image Note
TS3: compacted morula Present 2A mFgf4-/-; zFgf4+/- Merge , 2A mFgf4-/-; zFgf4+/- GATA6
Specimen 2A mFgf4-/-; zFgf4-/-: embryonic day 2.0; Fgf4tm1.2Mrt/Fgf4tm1.3Mrt, Tg(Zp3-cre)3Mrt/0 (more )
Note: Conditional mutant. 16 cells, morula stage. Double labeled: red - Gata6; white - Nanog (assay MGI:6756421).
Structure Level Pattern Image Note
TS3: compacted morula Present 2A mFgf4-/-; zFgf4-/- GATA6 , 2A mFgf4-/-; zFgf4-/- Merge
Specimen 2A zFGF4-/-: embryonic day 2.0; Fgf4tm1.3Mrt/Fgf4tm1.3Mrt (more )
Note: 14 cells, morula stage. Double labeled: red - Gata6; white - Nanog (assay MGI:6756421).
Structure Level Pattern Image Note
TS3: compacted morula Present 2A zFgf4-/- Merge , 2A zFgf4-/- GATA6
Specimen 2B mFgf4-/-; zFgf4+/- E3: embryonic day 3.0; Fgf4tm1.2Mrt/Fgf4+, Tg(Msx2-cre)5Rem/0 (more )
Note: Conditional mutant. 32 cells. Double labeled: red - Gata6; white - Nanog (assay MGI:6756421).
Structure Level Pattern Image Note
TS4: inner cell mass Present 2B mFgf4-/-; zFgf4+/- E3.0 Merge 3D , 2B mFgf4-/-; zFgf4+/- E3.0 GATA6 , 2B mFgf4-/-; zFgf4+/- E3.0 Merge 2D
Specimen 2B mFgf4-/-; zFgf4+/- E3.5: embryonic day 3.5; Fgf4tm1.2Mrt/Fgf4+, Tg(Msx2-cre)5Rem/0 (more )
Note: Conditional mutant. 52 cells. Double labeled: red - Gata6; white - Nanog (assay MGI:6756421).
Structure Level Pattern Image Note
TS5: inner cell mass Present 2B mFgf4-/-; zFgf4+/- E3.5 GATA6 , 2B mFgf4-/-; zFgf4+/- E3.5 Merge 2D , 2B mFgf4-/-; zFgf4+/- E3.5 Merge 3D
Specimen 2B mFgf4-/-; zFgf4-/- E3: embryonic day 3.0; Fgf4tm1.2Mrt/Fgf4tm1.3Mrt, Tg(Zp3-cre)3Mrt/0 (more )
Note: Conditional mutant. 32 cells. Double labeled: red - Gata6; white - Nanog (assay MGI:6756421).
Structure Level Pattern Image Note
TS4: inner cell mass Present 2B mFgf4-/-; zFgf4-/- E3.0 Merge 2D , 2B mFgf4-/-; zFgf4-/- E3.0 Merge 3D , 2B mFgf4-/-; zFgf4-/- E3.0 GATA6
Specimen 2B mFgf4-/-; zFgf4-/- E3.5: embryonic day 3.5; Fgf4tm1.2Mrt/Fgf4tm1.3Mrt, Tg(Zp3-cre)3Mrt/0 (more )
Note: Conditional mutant. 59 cells. Double labeled: red - Gata6; white - Nanog (assay MGI:6756421).
Structure Level Pattern Image Note
TS5: inner cell mass Present Single cells 2B mFgf4-/-; zFgf4-/- E3.5 GATA6 , 2B mFgf4-/-; zFgf4-/- E3.5 Merge 2D , 2B mFgf4-/-; zFgf4-/- E3.5 Merge 3D
Specimen S4A Fgf4+/+: embryonic day 3.5 (more )
Note: Double labeled: red - Gata6; white - Nanog (assay MGI:6756421).
Structure Level Pattern Image Note
TS5: inner cell mass Present S4A Fgf4+/+ GATA6 , S4A Fgf4+/+ Merge
Specimen S4A Fgf4-/-: embryonic day 3.5; Fgf4tm1.3Mrt/Fgf4tm1.3Mrt (more )
Note: The authors worked with two mutants that had identical phenotypes; it is possible this specimen carried Fgf4tm1Gol instead of Fgf4tm1.3Mrt. Double labeled: red - Gata6; white - Nanog (assay MGI:6756421).
Structure Level Pattern Image Note
TS5: inner cell mass Absent S4A Fgf4-/- GATA6 , S4A Fgf4-/- Merge
Specimen S4B Fgf4+/+: embryonic day 4.5 (more )
Note: Double labeled: red - Gata6; white - Nanog (assay MGI:6756421).
Structure Level Pattern Image Note
TS5: inner cell mass Present S4C Fgf4+/+ GATA6 , S4C Fgf4+/+ Merge
Specimen S4B Fgf4-/-: embryonic day 4.5; Fgf4tm1.3Mrt/Fgf4tm1.3Mrt (more )
Note: The authors worked with two mutants that had identical phenotypes; it is possible this specimen carried Fgf4tm1Gol instead of Fgf4tm1.3Mrt. Double labeled: red - Gata6; white - Nanog (assay MGI:6756421).
Structure Level Pattern Image Note
TS5: inner cell mass Absent S4C Fgf4-/- GATA6 , S4C Fgf4-/- Merge
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03/25/2025
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