Gene Expression Data
Assay Details
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Assay
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Reference:
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J:297659
Ge Y, et al., Two new genetically modified mouse alleles labeling distinct phases of retinal ganglion cell development by fluorescent proteins. Dev Dyn. 2020 Aug 2;
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6750900
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Gene symbol:
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Pou4f2
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Gene name:
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POU domain, class 4, transcription factor 2
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Reporter:
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tdTomato
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Antibody:
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anti-mCherry (tdTomato)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
3M/N/O:
embryonic day 14.5; Pou4f2tm2Xmu/Pou4f2 +
(more  )
Note: Double labeled: green - Pou4f2 tdTomato reporter (antibody); green - Pou4f2 tdTomato reporter (direct detection; assay MGI:6750871).
Specimen
3M/N/O:
(close  )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCrl
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Mutant Allele(s):
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Pou4f2tm2Xmu/Pou4f2+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Pou4f2 tdTomato reporter (antibody); green - Pou4f2 tdTomato reporter (direct detection; assay MGI:6750871).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: neural retina |
Present |
Regionally restricted |
3N
,
3O
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Expression is primarily seen in the ganglion cell layer. The antibody does detect some expressing cells in the neuroblast layer, suggesting they are newly born RGCs and that the reporter protein is not mature enough for fluorescence emission.
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Analysis Tools
