Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:297659
Ge Y, et al., Two new genetically modified mouse alleles labeling distinct phases of retinal ganglion cell development by fluorescent proteins. Dev Dyn. 2020 Aug 2;
|
|
Assay type:
|
In situ reporter (knock in)
|
|
MGI Accession ID:
|
MGI:6750898
|
|
Gene symbol:
|
Atoh7
|
|
Gene name:
|
atonal bHLH transcription factor 7
|
|
Reporter:
|
HA
|
|
Antibody:
|
anti-HA tag
|
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor 546
|
|
|
Results
|
Specimen
2E/F/G:
(close  )
|
Genetic Background:
|
involves: 129S6/SvEvTac * C57BL/6NCrl
|
|
Mutant Allele(s):
|
Atoh7tm1.1Xmu/Atoh7tm3(cre/ERT2)Xmu
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Atoh7 HA tag ; green - Atoh7 ZsGreen reporter (assay MGI:6750868).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: neural retina |
Present |
Regionally restricted |
2F
,
2F'
,
2G
,
2G'
|
Expression is seen in a subset of retinal progenitor cells in the neuroblast layer. In contrast with the reporter, very few expressing cells are found in the inner ganglion cell layer.
|
|
Analysis Tools
)
