Gene Expression Data
Assay Details
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Assay
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Reference:
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J:297659
Ge Y, et al., Two new genetically modified mouse alleles labeling distinct phases of retinal ganglion cell development by fluorescent proteins. Dev Dyn. 2020 Aug 2;
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6750871
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Gene symbol:
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Pou4f2
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Gene name:
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POU domain, class 4, transcription factor 2
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Reporter:
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tdTomato
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Direct Detection
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Results
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Specimen
1D:
embryonic day 14.5; Pou4f2tm2Xmu/Pou4f2 +
(more  )
Specimen
1D:
(close  )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCrl
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Mutant Allele(s):
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Pou4f2tm2Xmu/Pou4f2+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3A:
embryonic day 12.5; Pou4f2tm2Xmu/Pou4f2 +
(more )
Specimen
3A:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCrl
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Mutant Allele(s):
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Pou4f2tm2Xmu/Pou4f2+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: neural retina |
Present |
Regionally restricted |
3A
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Expression is confined to the ganglion cell layer of the central retina region.
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Specimen
3B:
embryonic day 14.5; Pou4f2tm2Xmu/Pou4f2 +
(more )
Specimen
3B:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCrl
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Mutant Allele(s):
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Pou4f2tm2Xmu/Pou4f2+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: neural retina |
Strong |
Regionally restricted |
3B
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Strong expression is seen in most, if not all, cells in the ganglion cell layer. It is also seen in the axons in the neural fiber layer.
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Specimen
3C:
embryonic day 17.5; Pou4f2tm2Xmu/Pou4f2 +
(more )
Specimen
3C:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCrl
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Mutant Allele(s):
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Pou4f2tm2Xmu/Pou4f2+
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: neural retina |
Strong |
Regionally restricted |
3C
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Strong expression is seen in most, if not all, cells in the ganglion cell layer.
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| TS25: retina nerve fiber layer |
Present |
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3C
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Expression is seen in the axons in the neural fiber layer.
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Specimen
not shown:
postnatal day 0; Pou4f2tm2Xmu/Pou4f2 +
(more )
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCrl
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Mutant Allele(s):
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Pou4f2tm2Xmu/Pou4f2+
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3D:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCrl
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Mutant Allele(s):
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Pou4f2tm2Xmu/Pou4f2+
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Age:
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postnatal day 5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina ganglion cell layer |
Present |
Regionally restricted |
3D
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Strong expression is only seen in a fraction of the retinal ganglion cells.
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Specimen
3E:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCrl
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Mutant Allele(s):
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Pou4f2tm2Xmu/Pou4f2+
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Age:
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postnatal day 16
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3F/G/H:
embryonic day 14.5; Pou4f2tm2Xmu/Pou4f2 +
(more )
Note: Double labeled: red - Pou4f2 reporter; green - Pou4f2 protein (assay MGI:6750887).
Specimen
3F/G/H:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCrl
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Mutant Allele(s):
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Pou4f2tm2Xmu/Pou4f2+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pou4f2 reporter; green - Pou4f2 protein (assay MGI:6750887).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: neural retina |
Present |
Regionally restricted |
3H
,
3F
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Expression is seen in the ganglion cell layer; reporter cells are not seen in the neuroblast layer.
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Specimen
3J/K/L:
embryonic day 14.5; Pou4f2tm2Xmu/Pou4f2 +
(more )
Note: Double labeled: red - Pou4f2 tdTomato reporter; green - Pou4f2 Flag tag (assay MGI:6750902).
Specimen
3J/K/L:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCrl
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Mutant Allele(s):
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Pou4f2tm2Xmu/Pou4f2+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pou4f2 tdTomato reporter; green - Pou4f2 Flag tag (assay MGI:6750902).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: neural retina |
Present |
Regionally restricted |
3L
,
3J
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Expression is seen in the ganglion cell layer; reporter cells are not seen in the neuroblast layer.
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Specimen
3M/N/O:
embryonic day 14.5; Pou4f2tm2Xmu/Pou4f2 +
(more )
Note: Double labeled: red - Pou4f2 tdTomato reporter (direct detection); green - Pou4f2 tdTomato reporter (antibody; assay MGI:6750900).
Specimen
3M/N/O:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCrl
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Mutant Allele(s):
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Pou4f2tm2Xmu/Pou4f2+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pou4f2 tdTomato reporter (direct detection); green - Pou4f2 tdTomato reporter (antibody; assay MGI:6750900).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: neural retina |
Present |
Regionally restricted |
3M
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3O
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Expression is seen in the ganglion cell layer; reporter cells are not seen in the neuroblast layer.
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Specimen
4A/B/C:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6NCrl
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Mutant Allele(s):
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Atoh7tm3(cre/ERT2)Xmu/Atoh7+
Pou4f2tm2Xmu/Pou4f2+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pou4f2; green - Atoh7 (assay MGI:6750868).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: neural retina |
Present |
Regionally restricted |
4C
,
4C'
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4B'
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4B
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Expression is primarily seen in the ganglion cell layer.
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Analysis Tools