Casp3 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:181048 Ghosh AP, et al., Deficiency of pro-apoptotic Hrk attenuates programmed cell death in the developing murine nervous system but does not affect Bcl-x deficiency-induced neuron apoptosis. J Histochem Cytochem. 2011 Nov;59(11):976-83
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6741142
Gene symbol:	Casp3
Gene name:	caspase 3

Antibody:	anti-cleaved Caspase-3 (Asp175) (#9661)
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	A tyramide signal amplification system was used. The antibody used recognizes the cleaved form of the protein.

Results

Specimen 1A: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: dorsal root ganglion	Present	Single cells	1A

Specimen 1B: embryonic day 15.5; Hrk^tm1Ast/Hrk^tm1Ast (more

)

Structure	Level	Pattern	Image	Note
TS23: dorsal root ganglion	Present	Single cells	1B	The number of cleaved caspase-3 immunoreactive cells in the immature neurons was significantly reduced in this mutant compared to wild-type.

Specimen 2B wild-type: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present	Single cells	2B wild-type

Specimen 2B Hrk-/-: embryonic day 12.5; Hrk^tm1Ast/Hrk^tm1Ast (more

)

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present	Single cells	2B Hrk-/-	The number of cleaved caspase-3 immunoreactive cells in the immature neurons was significantly reduced in this mutant compared to wild-type.

Specimen 2B Bcl-x-/-: embryonic day 12.5; Bcl2l1^tm1Dlo/Bcl2l1^tm1Dlo (more

)

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present	Single cells	2B Bcl-x-/-	The number of cleaved caspase-3 immunoreactive cells in the immature neurons was increased in this mutant compared to wild-type.

Specimen 2B Bcl-x-/-;Hrk-/-: embryonic day 12.5; Bcl2l1^tm1Dlo/Bcl2l1^tm1Dlo, Hrk^tm1Ast/Hrk^tm1Ast (more

)

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present	Single cells	2B Bcl-x-/-;Hrl-/-	The number of cleaved caspase-3 immunoreactive cells in the immature neurons was increased in this mutant compared to wild-type.

Specimen Table 2C wild-type: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present	Single cells

Specimen Table 2C Bim-/-: embryonic day 12.5; Bcl2l11^tm1Ast/Bcl2l11^tm1Ast (more

)

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present	Single cells		Expression in this mutant is similar to wild-type.

Specimen Table 2C Hrk-/-: embryonic day 12.5; Hrk^tm1Ast/Hrk^tm1Ast (more

)

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present	Single cells		The number of cleaved caspase-3 immunoreactive cells in the immature neurons was significantly reduced in this mutant compared to wild-type.

Specimen Table 2C Hrk-/-;Bim-/-: embryonic day 12.5; Bcl2l11^tm1Ast/Bcl2l11^tm1Ast, Hrk^tm1Ast/Hrk^tm1Ast (more

)

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present	Single cells		The number of cleaved caspase-3 immunoreactive cells in the immature neurons was significantly reduced in this mutant compared to wild-type.

Specimen Table 2C Bcl-x-/-: embryonic day 12.5; Bcl2l1^tm1Dlo/Bcl2l1^tm1Dlo (more

)

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present	Single cells		There is no significant difference in this mutant in comparison to wild-type controls.

Specimen Table 2C Hrk-/-;Bcl-x-/-: embryonic day 12.5; Bcl2l1^tm1Dlo/Bcl2l1^tm1Dlo, Hrk^tm1Ast/Hrk^tm1Ast (more

)

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present	Single cells		There is no significant difference in this mutant in comparison to wild-type controls.

Specimen Table 2C Hrk-/-;Bim-/-;Bcl-x-/-: embryonic day 12.5; Bcl2l1^tm1Dlo/Bcl2l1^tm1Dlo, Bcl2l11^tm1Ast/Bcl2l11^tm1Ast, Hrk^tm1Ast/Hrk^tm1Ast (more

)

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present	Single cells		There is no significant difference in this mutant in comparison to wild-type controls.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/24/2026 MGI 6.24