Wt1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:293793 Basta JM, et al., The core SWI/SNF catalytic subunit Brg1 regulates nephron progenitor cell proliferation and differentiation. Dev Biol. 2020 Aug 15;464(2):176-187
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6740181
Gene symbol:	Wt1
Gene name:	WT1 transcription factor

Antibody:	anti-WT1 [CAN-R9(IHC)-56-2; ab89901]
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

Specimen 5A Wt1 Brg1flx/flx: embryonic day 16.5; Smarca4^tm1.2Pcn/Smarca4^tm1.2Pcn (more

)
Note: Triple labeled: green - Wt1; red - Ncam1 (assay MGI:6740176); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS24: maturing glomerular tuft	Present		5A Brg1flx/flx Wt1/NCAM	Formation of immature glomeruli readily apparent.

Specimen 5A Wt1 Six2Cre Brg1flx/flx: embryonic day 16.5; Smarca4^tm1.2Pcn/Smarca4^tm1.2Pcn, Tg(Six2-EGFP/cre)1Amc/0 (more

)
Note: Conditional mutant. Triple labeled: green - Wt1; red - Ncam1 (assay MGI:6740176); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS24: maturing glomerular tuft	Weak		5A Six2Cre Brg1flx/flx Wt1/NCAM	Authors report a 50% reduction in glomeruli.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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