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Gene Expression Data
Assay Details
Assay
Reference: J:149312 Moran JL, et al., Manic fringe is not required for embryonic development, and fringe family members do not exhibit redundant functions in the axial skeleton, limb, or hindbrain. Dev Dyn. 2009 May 28;238(7):1803-1812
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6729894
Gene symbol: Rfng
Gene name: RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Reporter: lacZ
Direct Detection
Results
Specimen 3Ba: embryonic day 9.5; Rfngtm1Tfv/Rfng+ (more )
Structure Level Pattern Image Note
TS15: limb bud Present 3Ba
Specimen 3Bb: embryonic day 9.5; En1tm1Alj/En1tm1Alj, Rfngtm1Tfv/Rfng+ (more )
Structure Level Pattern Image Note
TS15: limb bud Present 3Bb Similar as in the control.
Specimen 3Ca: embryonic day 10.5; Rfngtm1Tfv/Rfng+ (more )
Structure Level Pattern Image Note
TS17: limb ectoderm Present 3Ca Expressed in the dorsal ectoderm and ventral ectoderm.
TS17: limb mesenchyme Present 3Ca
Specimen 3Cb: embryonic day 10.5; En1tm1Alj/En1+, Rfngtm1Tfv/Rfngtm1Tfv (more )
Structure Level Pattern Image Note
TS17: limb ectoderm Present 3Cb Expressed in the dorsal ectoderm and ventral ectoderm.
TS17: limb mesenchyme Present 3Cb
Specimen 3Cc: embryonic day 10.5; En1tm1Alj/En1tm1Alj, Rfngtm1Tfv/Rfng+ (more )
Structure Level Pattern Image Note
TS17: limb ectoderm Present 3Cc Expressed in the dorsal ectoderm and ventral ectoderm.
TS17: limb mesenchyme Present 3Cc
TS17: apical ectodermal ridge Present 3Cc Thickened at the limb apex.
Specimen 3Cd: embryonic day 10.5; En1tm1Alj/En1tm1Alj, Rfngtm1Tfv/Rfngtm1Tfv (more )
Structure Level Pattern Image Note
TS17: limb ectoderm Present 3Cd Expressed in the dorsal ectoderm and ventral ectoderm.
TS17: limb mesenchyme Present 3Cd
TS17: apical ectodermal ridge Present 3Cd Thickened at the limb apex.
Specimen 3Da: embryonic day 10.5; Rfngtm1Tfv/Rfng+ (more )
Structure Level Pattern Image Note
TS17: apical ectodermal ridge Present 3Da
Specimen 3Db: embryonic day 10.5; En1tm1Alj/En1+, Rfngtm1Tfv/Rfngtm1Tfv (more )
Structure Level Pattern Image Note
TS17: apical ectodermal ridge Present 3Db
Specimen 3Dc: embryonic day 10.5; En1tm1Alj/En1tm1Alj, Rfngtm1Tfv/Rfng+ (more )
Structure Level Pattern Image Note
TS17: apical ectodermal ridge Present 3Dc Ventrally expanded and flattened.
Specimen 3Dd: embryonic day 10.5; En1tm1Alj/En1tm1Alj, Rfngtm1Tfv/Rfngtm1Tfv (more )
Structure Level Pattern Image Note
TS17: apical ectodermal ridge Present 3Dd Ventrally expanded and flattened, similar as in the R+/-;En-/-.
Specimen 3Ea: embryonic day 11.5; Rfngtm1Tfv/Rfng+ (more )
Structure Level Pattern Image Note
TS19: apical ectodermal ridge Present 3Ea
Specimen 3Eb: embryonic day 11.5; En1tm1Alj/En1+, Rfngtm1Tfv/Rfngtm1Tfv (more )
Structure Level Pattern Image Note
TS19: apical ectodermal ridge Present 3Eb
Specimen 3Ec: embryonic day 11.5; En1tm1Alj/En1tm1Alj, Rfngtm1Tfv/Rfng+ (more )
Structure Level Pattern Image Note
TS19: apical ectodermal ridge Present 3Ec Flattened and expanded, with frequent bifurcations, as well as nubs on the ventral anterior surface.
Specimen 3Ed: embryonic day 11.5; En1tm1Alj/En1tm1Alj, Rfngtm1Tfv/Rfngtm1Tfv (more )
Structure Level Pattern Image Note
TS19: apical ectodermal ridge Present 3Ed Flattened and expanded, with frequent bifurcations, as well as nubs on the ventral anterior surface. Similar as in the R+/-;En-/-.
Specimen 3Fa: embryonic day 11.5; Rfngtm1Tfv/Rfng+ (more )
Structure Level Pattern Image Note
TS19: apical ectodermal ridge Present 3Fa
Specimen 3Fb: embryonic day 11.5; En1tm1Alj/En1tm1Alj, Rfngtm1Tfv/Rfng+ (more )
Structure Level Pattern Image Note
TS19: apical ectodermal ridge Present 3Fb
Specimen 3Fc: embryonic day 11.5; En1tm1Alj/En1tm1Alj, Rfngtm1Tfv/Rfng+ (more )
Structure Level Pattern Image Note
TS19: apical ectodermal ridge Present 3Fc Flattened and expanded, with frequent bifurcations, as well as nubs on the ventral anterior surface.
Specimen 3Fd: embryonic day 11.5; En1tm1Alj/En1tm1Alj, Rfngtm1Tfv/Rfngtm1Tfv (more )
Structure Level Pattern Image Note
TS19: apical ectodermal ridge Present 3Fd Flattened and expanded, with frequent bifurcations, as well as nubs on the ventral anterior surface. Similar as in the R+/-;En-/-.
Specimen S2A +/-: embryonic day 7.5; Rfngtm1Tfv/Rfng+ (more )
Structure Level Pattern Image Note
TS11: embryo Present S2A left
TS11: extraembryonic component Present S2A left
Specimen S2A -/-: embryonic day 7.5; Rfngtm1Tfv/Rfngtm1Tfv (more )
Structure Level Pattern Image Note
TS11: embryo Present Widespread S2A right
TS11: extraembryonic component Present Widespread S2A right
Specimen S2B: embryonic day 7.5; Rfngtm1Tfv/Rfngtm1Tfv (more )
Structure Level Pattern Image Note
TS11: embryo Present Widespread S2B
TS11: extraembryonic component Present Widespread S2B
Specimen S2C: embryonic day 8.5; Rfngtm1Tfv/Rfngtm1Tfv (more )
Structure Level Pattern Image Note
TS13: branchial arch Present S2C
TS13: future hindbrain Present S2C
TS13: future midbrain Present S2C
TS13: future spinal cord Present S2C
TS13: optic pit Present S2C
TS13: somite Present S2C
Specimen S2D: embryonic day 8.5; Rfngtm1Tfv/Rfngtm1Tfv (more )
Structure Level Pattern Image Note
TS13: branchial arch Present S2D
TS13: future hindbrain Present S2D
TS13: future midbrain Present S2D
TS13: future spinal cord Present S2D
TS13: optic pit Present S2D
TS13: somite Present S2D
Specimen S2E: embryonic day 9.5; Rfngtm1Tfv/Rfngtm1Tfv (more )
Structure Level Pattern Image Note
TS15: branchial arch Present Widespread S2E
TS15: future spinal cord Present Widespread S2E
TS15: limb bud Present Widespread S2E
TS15: midbrain-hindbrain junction Present Widespread S2E
TS15: somite Present Widespread S2E
TS15: peripheral nervous system Present Regionally restricted S2E Expressed in trigeminal and facial nerves.
Specimen S2F: embryonic day 10.5; Rfngtm1Tfv/Rfngtm1Tfv (more )
Structure Level Pattern Image Note
TS17: branchial arch Present S2F
TS17: dorsal root ganglion Present S2F
TS17: forebrain Present S2F
TS17: forebrain-midbrain boundary region Present S2F
TS17: future spinal cord Present S2F
TS17: heart Present S2F
TS17: limb bud Present S2F
TS17: midbrain Present S2F
TS17: nasal pit Present S2F
TS17: spinal nerve Present S2F
TS17: cranial nerve Present Regionally restricted S2F Expressed in the trigeminal, facial, glossopharyngeal, and vagus nerves.
Specimen S2G: embryonic day 12.5; Rfngtm1Tfv/Rfngtm1Tfv (more )
Structure Level Pattern Image Note
TS20: brain Present S2G
TS20: facial nerve Present S2G
TS20: glossopharyngeal nerve Present S2G
TS20: heart Present S2G
TS20: limb Present S2G
TS20: mammary gland primordium Present S2G
TS20: nasal cavity Present S2G
TS20: spinal cord Present S2G
TS20: trigeminal nerve Present S2G
TS20: vagus nerve Present S2G
Specimen S2H: embryonic day 9.5; Rfngtm1Tfv/Rfngtm1Tfv (more )
Structure Level Pattern Image Note
TS15: neural tube lateral wall Present S2H
TS15: notochord Present S2H
Specimen S2I: embryonic day 10.5; Rfngtm1Tfv/Rfngtm1Tfv (more )
Structure Level Pattern Image Note
TS17: neural tube floor plate Present S2I
TS17: notochord Present S2I
TS17: neural tube lateral wall Strong Regionally restricted S2I Weak expression in the ventricular zone and strong expression in the mantle zone.
TS17: dorsal root ganglion Strong S2I
TS17: spinal ganglion Strong S2I
Specimen S2J: embryonic day 12.5; Rfngtm1Tfv/Rfngtm1Tfv (more )
Structure Level Pattern Image Note
TS20: spinal cord floor plate Present S2J
TS20: spinal cord mantle layer Present S2J
TS20: spinal cord lateral wall Present Regionally restricted S2J Expressed in the ventral neural tube, apparently correlating with the differentiation of motor neurons and/or ventral interneurons.
Specimen S2K: embryonic day 11.5; Rfngtm1Tfv/Rfngtm1Tfv (more )
Structure Level Pattern Image Note
TS19: embryo ectoderm Present S2K After prolonged staining.
Specimen S2L: embryonic day 13.5; Rfngtm1Tfv/Rfngtm1Tfv (more )
Structure Level Pattern Image Note
TS21: lens Present S2L
Specimen S2M: embryonic day 13.5; Rfngtm1Tfv/Rfngtm1Tfv (more )
Structure Level Pattern Image Note
TS21: mammary gland primordium Weak S2M
TS21: vibrissa Weak S2M
TS21: limb epithelium Weak Regionally restricted S2M Expressed in limb ectoderm and apical ectodermal ridge.
Specimen S2N: embryonic day 13.5; Rfngtm1Tfv/Rfngtm1Tfv (more )
Structure Level Pattern Image Note
TS21: rib cartilage condensation Present S2N
TS21: vertebral cartilage condensation Present S2N
Specimen S2O: embryonic day 17.5; Rfngtm1Tfv/Rfngtm1Tfv (more )
Structure Level Pattern Image Note
TS25: vibrissa Present S2O
Specimen S2P: embryonic day 17.5; Rfngtm1Tfv/Rfngtm1Tfv (more )
Structure Level Pattern Image Note
TS25: hair follicle Present S2P
Specimen S2Q: postnatal newborn; Rfngtm1Tfv/Rfngtm1Tfv (more )
Structure Level Pattern Image Note
TS27: hair follicle Present S2Q
Specimen S3A/B: postnatal adult; Rfngtm1Tfv/Rfngtm1Tfv (more )
Structure Level Pattern Image Note
TS28: hippocampus Present S3A
TS28: thalamus Present S3A
TS28: cerebral cortex Present S3B , S3A
Specimen S3C/D: postnatal adult; Rfngtm1Tfv/Rfngtm1Tfv (more )
Structure Level Pattern Image Note
TS28: corpus striatum Present S3C , S3D
TS28: globus pallidus Present S3C , S3D
TS28: hypothalamus Present S3C
Specimen S3E/F: postnatal adult; Rfngtm1Tfv/Rfngtm1Tfv (more )
Structure Level Pattern Image Note
TS28: anterior olfactory nucleus Present S3E
TS28: inferior colliculus Present S3E
TS28: superior colliculus Present S3E
TS28: dentate gyrus Present S3E , S3F Expression in the CA1 region and dentate gyrus were more intense than expression in the CA3 region.
TS28: hippocampus CA1 Present S3E , S3F Expression in the CA1 region and dentate gyrus were more intense than expression in the CA3 region.
TS28: hippocampus CA3 Present S3F , S3E
Specimen S3G/H: postnatal adult; Rfngtm1Tfv/Rfngtm1Tfv (more )
Structure Level Pattern Image Note
TS28: anterior olfactory nucleus Present S3G
TS28: hypothalamus Present S3G
TS28: inferior colliculus Present S3G
TS28: main olfactory bulb Present S3G
TS28: pons Present S3G
TS28: superior colliculus Present S3G
TS28: thalamus Present S3G
TS28: cerebellum Purkinje cell layer Present S3G , S3H
TS28: cerebellum internal granule cell layer Present S3G , S3H
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory