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Gene Expression Data
Assay Details
Assay
Reference: J:278517 Li M, et al., The histone modification reader ZCWPW1 is required for meiosis prophase I in male but not in female mice. Sci Adv. 2019;5(8):eaax1101
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6727336
Gene symbol: H2ax
Gene name: H2A.X variant histone
Antibody: anti-phosphoH2AX (#05-636; JBW301)
Detection system: Alexa Fluor 488
Results
Specimen 3M: postnatal day 25 (more )
Note: Leptotene stage. Double labeled: green - H2ax; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 3M Expression in chromatin domains surrounding double strand breaks (DSB).
Specimen 3N: postnatal day 25 (more )
Note: Zygotene stage. Double labeled: green - H2ax; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 3N Expression in chromatin domains surrounding double strand breaks (DSB).
Specimen 3O: postnatal day 25 (more )
Note: Pachytene stage. Double labeled: green - H2ax; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 3O Expression only in XY bodies.
Specimen 3P: postnatal day 25; Zcwpw1em1Kliu/Zcwpw1em1Kliu (more )
Note: Leptotene stage. Double labeled: green - H2ax; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 3P Expression in chromatin domains surrounding double strand breaks (DSB).
Specimen 3Q: postnatal day 25; Zcwpw1em1Kliu/Zcwpw1em1Kliu (more )
Note: Zygotene stage. Double labeled: green - H2ax; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 3Q Expression in chromatin domains surrounding double strand breaks (DSB).
Specimen 3R: postnatal day 25; Zcwpw1em1Kliu/Zcwpw1em1Kliu (more )
Note: Pachytene-like. Double labeled: green - H2ax; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 3R Expression persisted on chromatin of unsynapsed axes.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory