Gene Expression Data
Assay Details
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Assay
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Reference:
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J:228503
Altieri SC, et al., En1 directs superior olivary complex neuron positioning, survival, and expression of FoxP1. Dev Biol. 2015 Dec 1;408(1):99-108
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6725817
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Gene symbol:
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Slc6a5
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Gene name:
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solute carrier family 6 (neurotransmitter transporter, glycine), member 5
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Probe:
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Glyt2 probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3C:
postnatal adult
(more  )
Specimen
3C:
(close  )
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Genetic Background:
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involves: 129S2/SvPas * 129S6/SvEvTac * C57BL/6J
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: superior olivary nucleus |
Present |
Regionally restricted |
3C
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Expressed in the glycinergic neurons in the lateral superior olive and superior paraolivary nucleus.
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| TS28: nucleus of trapezoid body |
Present |
Regionally restricted |
3C
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Expressed in the glycinergic neurons in the lateral, medial and ventral nuclei.
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Specimen
3D:
(close )
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Genetic Background:
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involves: 129S2/SvPas * 129S6/SvEvTac * C57BL/6J
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Mutant Allele(s):
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Egr2tm2(cre)Pch/Egr2+
En1tm8.1Alj/En1tm8.1Alj
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Conditional mutant.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: superior olivary nucleus |
Present |
Regionally restricted |
3D
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Expression in the glycinergic neurons in the lateral superior olive was completely absent, but expression in the superior paraolivary nucleus remained.
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| TS28: nucleus of trapezoid body |
Present |
Regionally restricted |
3D
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Expression in the glycinergic neurons in the medial and ventral nuclei were completely absent, but expression in the lateral nucleus remained.
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Analysis Tools