Ddx4 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:253477 Bailey AS, et al., The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline. Elife. 2017 Oct 31;6:e26116
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6724548
Gene symbol:	Ddx4
Gene name:	DEAD box helicase 4

Antibody:	anti-VASA (AF2030)
Detection system:	Alexa Fluor

Results

1 S1A' ©

Specimen 1-S1A: postnatal day 10 (more

)
Note: Triple labeled: red - Ddx4; green - Ythdc2 (assay MGI:6724542); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Present		1 S1A'

1 S1B' ©

Specimen 1-S1B: postnatal day 12 (more

)
Note: Triple labeled: red - Ddx4; green - Ythdc2 (assay MGI:6724542); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Present		1 S1B'

1 S1C' ©

Specimen 1-S1C: postnatal day 14 (more

)
Note: Triple labeled: red - Ddx4; green - Ythdc2 (assay MGI:6724542); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Present		1 S1C'

4 S1C ©

Specimen 4-S1C: postnatal day 12 (more

)
Note: Double labeled: green - Ddx4; red - Dmc1 (assay MGI:6724552).

Structure	Level	Pattern	Image	Note
TS28: testis	Present		4 S1C

4 S1D ©

Specimen 4-S1D: postnatal day 12; Ythdc2^{tm1b(EUCOMM)Hmgu}/Ythdc2^{tm1b(EUCOMM)Hmgu} (more

)
Note: Double labeled: green - Ddx4; red - Dmc1 (assay MGI:6724552).

Structure	Level	Pattern	Image	Note
TS28: testis	Present		4 S1D

5 S1E ©

Specimen 5-S1E: postnatal day 0.5 (more

)

Structure	Level	Pattern	Image	Note
TS27: germ cell of ovary	Present		5 S1E

5 S1F ©

Specimen 5-S1F: postnatal day 0.5; Ythdc2^{tm1b(EUCOMM)Hmgu}/Ythdc2^{tm1b(EUCOMM)Hmgu} (more

)

Structure	Level	Pattern	Image	Note
TS27: germ cell of ovary	Present		5 S1F	Expresion in premeiotic germ cells that appeared similar to those in wild type

5 S1J ©

Specimen 5-S1J: embryonic day 15.5 (more

)
Note: Triple labeled: green - Ddx4; red - Sycp3 (assay MGI:6724544).\; blue - DAPI.

Structure	Level	Pattern	Image	Note
TS23: germ cell of ovary	Present		5 S1J

5 S1K ©

Specimen 5-S1K: embryonic day 15.5; Ythdc2^{tm1b(EUCOMM)Hmgu}/Ythdc2^{tm1b(EUCOMM)Hmgu} (more

)
Note: Triple labeled: green - Ddx4; red - Sycp3 (assay MGI:6724544).\; blue - DAPI.

Structure	Level	Pattern	Image	Note
TS23: germ cell of ovary	Present		5 S1K

5M ©

Specimen 5M: embryonic day 15.5 (more

)
Note: Double labeled: green - Ddx4; red - Dmc1 (assay MGI:6724552).

Structure	Level	Pattern	Image	Note
TS23: germ cell of ovary	Present		5M

5N ©

Specimen 5N: embryonic day 15.5; Ythdc2^{tm1b(EUCOMM)Hmgu}/Ythdc2^{tm1b(EUCOMM)Hmgu} (more

)
Note: Double labeled: green - Ddx4; red - Dmc1 (assay MGI:6724552).

Structure	Level	Pattern	Image	Note
TS23: germ cell of ovary	Present		5N

5O ©

Specimen 5O: embryonic day 15.5 (more

)
Note: Double labeled: green - Ddx4; red - CyclinD1 (Ccnd1 assay MGI:6724562).

Structure	Level	Pattern	Image	Note
TS23: germ cell of ovary	Present		5O

5P ©

Specimen 5P: embryonic day 15.5; Ythdc2^{tm1b(EUCOMM)Hmgu}/Ythdc2^{tm1b(EUCOMM)Hmgu} (more

)
Note: Double labeled: green - Ddx4; red - CyclinD1 (Ccnd1 assay MGI:6724562).

Structure	Level	Pattern	Image	Note
TS23: germ cell of ovary	Present		5P

5Q ©

Specimen 5Q: embryonic day 16.5 (more

)
Note: Double labeled: green - Ddx4; red - phospho-Histone H3 (Thr3).

Structure	Level	Pattern	Image	Note
TS24: germ cell of ovary	Present		5Q

5R ©

Specimen 5R: embryonic day 16.5; Ythdc2^{tm1b(EUCOMM)Hmgu}/Ythdc2^{tm1b(EUCOMM)Hmgu} (more

)
Note: Double labeled: green - Ddx4; red - phospho-Histone H3 (Thr3).

Structure	Level	Pattern	Image	Note
TS24: germ cell of ovary	Present		5R

5 S2C ©

Specimen 5-S2C: postnatal day 12 (more

)
Note: Double labeled: green - Ddx4; red - Meioc (assay MGI:6724563).

Structure	Level	Pattern	Image	Note
TS28: testis	Present		5 S2C

5 S2D ©

Specimen 5-S2D: postnatal day 12; Ythdc2^{tm1b(EUCOMM)Hmgu}/Ythdc2^{tm1b(EUCOMM)Hmgu} (more

)
Note: Double labeled: green - Ddx4; red - Meioc (assay MGI:6724563).

Structure	Level	Pattern	Image	Note
TS28: testis	Present		5 S2D

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24