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Gene Expression Data
Assay Details
Assay
Reference: J:256775 Zhang H, et al., Endocardium Minimally Contributes to Coronary Endothelium in the Embryonic Ventricular Free Walls. Circ Res. 2016 Jun 10;118(12):1880-93
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6724010
Gene symbol: Npr3
Gene name: natriuretic peptide receptor 3
Reporter: ESR
Antibody: anti-ESR (ab27595)
Detection system: Secondary antibody coupled to Alexa Fluor 555
Results
Specimen 5A: embryonic day 9.5; Npr3em1(cre/ERT2)Bzsh/Npr3+ (more )
Note: Double labeled: red - Npr3 reporter; green - Cdh5 (assay MGI:6723818).
Structure Level Pattern Image Note
TS15: primitive ventricle endocardial lining Present 5A1 , 5A merge , 5A red
TS15: common atrial chamber endocardial lining Present 5A2 , 5A red , 5A merge
TS15: sinus venosus Absent 5A merge , 5A2 , 5A red
TS15: dorsal aorta Present Regionally restricted 5A merge , 5A red Expression is seen in endothelial cells.
Specimen 5B: embryonic day 10.5; Npr3em1(cre/ERT2)Bzsh/Npr3+ (more )
Note: Double labeled: red - Npr3 reporter; green - Cdh5 (assay MGI:6723818).
Structure Level Pattern Image Note
TS17: primitive ventricle endocardial lining Present 5B merge , 5B red , 5B1
TS17: common atrial chamber endocardial lining Present 5B2 , 5B red , 5B merge
TS17: sinus venosus Absent 5B2 , 5B red , 5B merge
TS17: dorsal aorta Present Regionally restricted 5B red , 5B merge Expression is seen in endothelial cells.
Specimen 5C: embryonic day 11.5; Npr3em1(cre/ERT2)Bzsh/Npr3+ (more )
Note: Double labeled: red - Npr3 reporter; green - Cdh5 (assay MGI:6723818).
Structure Level Pattern Image Note
TS19: ventricle endocardial lining Present 5C1 , 5C merge , 5C red
TS19: atrium endocardial lining Present 5C2 , 5C merge , 5C red
TS19: sinus venosus Absent 5C merge , 5C2 , 5C red
TS19: heart mesenchyme Present Regionally restricted 5C merge , 5C red Expression is seen in a subset of nonendothelial mesenchymal cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory