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Gene Expression Data
Assay Details
Assay
Reference: J:256775 Zhang H, et al., Endocardium Minimally Contributes to Coronary Endothelium in the Embryonic Ventricular Free Walls. Circ Res. 2016 Jun 10;118(12):1880-93
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6723822
Gene symbol: Pecam1
Gene name: platelet/endothelial cell adhesion molecule 1
Antibody: MEC 13.3
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 2B: embryonic day 10.5; Nfatc1em3(dre)Bzsh/Nfatc1+, Gt(ROSA)26Sortm66.2(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: green - Pecam1; red - Cre reporter.
Structure Level Pattern Image Note
TS17: atrium endocardial lining Present 2B right , 2B left Expression is seen in endothelial cells.
TS17: sinus venosus Present Regionally restricted 2B right , 2B left Expression is seen in endothelial cells.
TS17: primitive ventricle endocardial lining Present 2B left Expression is seen in endothelial cells.
TS17: heart endothelium Present 2B right , 2B left Expression is seen in endothelial cells.
Specimen 2C: embryonic day 11.5; Nfatc1em3(dre)Bzsh/Nfatc1+, Gt(ROSA)26Sortm66.2(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: green - Pecam1; red - Cre reporter.
Structure Level Pattern Image Note
TS19: atrium endocardial lining Present 2C right , 2C left Expression is seen in endothelial cells.
TS19: sinus venosus Present Regionally restricted 2C right , 2C left Expression is seen in endothelial cells.
TS19: ventricle endocardial lining Present 2C right Expression is seen in endothelial cells.
TS19: heart endothelium Present 2E left , 2C right Expression is seen in endothelial cells.
Specimen 2E: embryonic day 12.5; Nfatc1em3(dre)Bzsh/Nfatc1+, Gt(ROSA)26Sortm66.2(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: green - Pecam1; red - Cre reporter.
Structure Level Pattern Image Note
TS20: left ventricle endocardial lining Present 2E left Expression is seen in endothelial cells.
TS20: right ventricle endocardial lining Present 2E left Expression is seen in endothelial cells.
TS20: heart blood vessel Present Regionally restricted 2E right , 2E left Expression is seen in endothelial cells.
TS20: heart trabecula Present Regionally restricted 2E right , 2E left Expression is seen in endothelial cells.
TS20: heart endothelium Present 2E left , 2E right Expression is seen in endothelial cells.
Specimen 2F: embryonic day 15.5; Nfatc1em3(dre)Bzsh/Nfatc1+, Gt(ROSA)26Sortm66.2(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: green - Pecam1; red - Cre reporter.
Structure Level Pattern Image Note
TS23: right ventricle endocardial lining Present 2F merge , 2F1 merge , 2F green , 2F1 green Expression is seen in endothelial cells.
TS23: left ventricle endocardial lining Present 2F merge , 2F2 merge , 2F green , 2F2 green Expression is seen in endothelial cells.
TS23: heart blood vessel Present 2F1 merge , 2F2 merge , 2F2 green , 2F merge , 2F1 green , 2F green Expression is seen in endothelial cells.
TS23: heart trabecula Present 2F1 merge , 2F merge , 2F2 merge , 2F2 green , 2F green , 2F1 green Expression is seen in endothelial cells.
TS23: heart endothelium Present 2F merge , 2F2 merge , 2F1 merge , 2F1 green , 2F2 green , 2F green Expression is seen in endothelial cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory