Probe:	Ctgf cDNA3
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	The probe was hydrolyzed to approximately 250 nucleotides.

Structure	Level	Pattern	Image	Note
TS20: arterial blood vessel	Strong		1A
TS20: dorsal aorta	Strong		1A
TS20: heart left ventricle	Strong		1A
TS20: heart right ventricle	Strong		1A
TS20: cartilage	Strong	Regionally restricted	1A	Expression is in outer parts of early cartilage just condensing from the mesenchyme.
TS20: brain	Present		1A
TS20: connective tissue	Present		1A
TS20: mystacial pad	Present		1A
TS20: vein	Weak		1A
TS20: venous blood vessel	Weak		1A
TS20: intestine	Trace		1A
TS20: liver	Trace		1A
TS20: metanephros	Trace		1A
TS20: left atrium endocardial lining	Present	Single cells	1A
TS20: right atrium endocardial lining	Present	Single cells	1A
TS20: left atrium cardiac muscle	Absent		1A
TS20: right atrium cardiac muscle	Absent		1A

Structure	Level	Pattern	Image	Note
TS22: arterial blood vessel	Strong		1C
TS22: connective tissue	Strong		1C
TS22: heart left ventricle	Strong		1C
TS22: heart right ventricle	Strong		1C
TS22: mystacial pad	Strong		1C
TS22: cartilage	Strong	Regionally restricted	1C	Expression is in outer parts of early cartilage just condensing from the mesenchyme.
TS22: liver vasculature	Present		1C
TS22: venous blood vessel	Weak		1C
TS22: intestine	Trace		1C
TS22: liver	Trace		1C
TS22: metanephros	Trace		1C
TS22: left atrium endocardial lining	Present	Single cells	1C
TS22: right atrium endocardial lining	Present	Single cells	1C
TS22: stomach mesenchyme	Present	Regionally restricted	1C	Expression is stronger in the ventral part.
TS22: duodenum mesenchyme	Present	Regionally restricted	1C	Expression is stronger in the beginning.
TS22: left atrium cardiac muscle	Absent		1C
TS22: liver parenchyma	Absent		1C
TS22: right atrium cardiac muscle	Absent		1C

Structure	Level	Pattern	Image	Note
TS26: arterial blood vessel	Strong		1E
TS26: cartilage	Strong	Regionally restricted	1E	Expression is in outer parts of early cartilage just condensing from the mesenchyme.
TS26: cerebral cortex	Present		1E
TS26: connective tissue	Present		1E
TS26: main olfactory bulb	Present		1E
TS26: mystacial pad	Present		1E
TS26: intestine	Weak
TS26: liver	Weak
TS26: metanephros	Weak
TS26: venous blood vessel	Weak

Structure	Level	Pattern	Image	Note
TS22: artery endothelium	Strong		2A
TS22: dorsal aorta	Strong		2A
TS22: arterial system smooth muscle	Present	Single cells	2A	Expression is in a few infiltrating cells and is absent in the external part.

Structure	Level	Pattern	Image	Note
TS22: arterial blood vessel	Strong		2B
TS22: artery endothelium	Strong		2B
TS22: vena cava	Present	Single cells	2B	Expression is in an isolated endothelial cell in the vena cava.

Structure	Level	Pattern	Image	Note
TS20: interventricular septum	Present		2C
TS20: ventricle endocardial lining	Present		2C
TS20: atrium endocardial lining	Present	Single cells	2C

Structure	Level	Pattern	Image	Note
TS20: vertebral cartilage condensation	Strong	Regionally restricted	2D	Expression is in the condensing cartilage and is weak in the core.

Structure	Level	Pattern	Image	Note
TS24: vertebra	Present	Regionally restricted	2E	Expression is in mesenchymal tissue lining the inner surface of the bone and connective tissue surrounding the pulpous disc.
TS24: perichondrium	Present		2E
TS24: periosteum	Present		2E

Structure	Level	Pattern	Image	Note
TS22: connective tissue	Present	Regionally restricted	2F	Expression is in and around the rib cartilage in the surrounding connective tissue.
TS22: rib cartilage condensation	Present	Regionally restricted	2F	Expression is in and around the rib cartilage in the surrounding connective tissue.
TS22: perichondrium	Present		2F
TS22: periosteum	Present		2F

Structure	Level	Pattern	Image	Note
TS27: connective tissue	Strong	Regionally restricted	2G	Expression is in connective tissue lining conchal cartilage.
TS27: turbinate bone	Strong	Regionally restricted	2G	Expression is in connective tissue lining conchal cartilage.
TS27: perichondrium	Strong	Regionally restricted	2G	Expression is on the nasal bones.
TS27: periosteum	Present		2G
TS27: nasal epithelium	Absent		2G

Structure	Level	Pattern	Image	Note
TS26: femur	Present	Regionally restricted	2H	Expression is in the zone of cell death and in the innerbone connective tissue cells, and is absent in the ossifying bone.
TS26: blood vessel	Present		2H
TS26: perichondrium	Present		2H
TS26: periosteum	Present		2H
TS26: intestine epithelium	Absent		2H
TS26: upper leg muscle	Absent		2H

Structure	Level	Pattern	Image	Note
TS24: connective tissue	Present	Regionally restricted	3A	Expression is in the connective tissue lining both sides of the muscularis propria main smooth muscle layer and in the subepithelial mesenchyme in the ventral part of the stomach and is weaker posteriorly.
TS24: stomach mesenchyme	Present	Regionally restricted	3A	Expression is in the connective tissue lining both sides of the muscularis propria main smooth muscle layer and in the subepithelial mesenchyme in the ventral part of the stomach and is weaker posteriorly.
TS24: duodenum mesenchyme	Present	Regionally restricted	3A	Expression is stronger in the beginning.
TS24: stomach epithelium	Absent		3A
TS24: stomach muscularis externa	Absent		3A

Structure	Level	Pattern	Image	Note
TS22: intestine mesenchyme	Moderate	Regionally restricted	3B	Expression is in mesenchyme on both sides of the smooth muscle layer.
TS22: intestine epithelium	Absent		3B

Structure	Level	Pattern	Image	Note
TS20: lung mesenchyme	Present		3C
TS20: lung epithelium	Absent		3C

Structure	Level	Pattern	Image	Note
TS22: blood vessel endothelium	Strong	Regionally restricted	3D	Expression is in cells lining small vessels in the lung.
TS22: lung vascular element	Strong	Regionally restricted	3D	Expression is in cells lining small vessels in the lung.
TS22: vena cava	Weak	Single cells	3D	Expression is in an isolated endothelial cell in the vena cava.
TS22: lung epithelium	Absent		3D

Structure	Level	Pattern	Image	Note
TS26: arterial system endothelium	Strong	Regionally restricted	3E	Expression is in cells lining small vessels in the lung.
TS26: blood vessel endothelium	Strong	Regionally restricted	3E	Expression is in cells lining small vessels in the lung.
TS26: lung vascular element	Strong	Regionally restricted	3E	Expression is in arteries.
TS26: venous blood vessel	Weak	Single cells	3E	Expression is in an isolated endothelial cell.
TS26: lung epithelium	Absent		3E

Structure	Level	Pattern	Image	Note
TS26: arterial system endothelium	Strong	Regionally restricted	3F	Expression is in cells lining small vessels in the lung.
TS26: blood vessel endothelium	Strong	Regionally restricted	3F	Expression is in cells lining small vessels in the lung.
TS26: lung vascular element	Strong	Regionally restricted	3F	Expression is in cells lining small vessels in the lung.

Structure	Level	Pattern	Image	Note
TS24: blood vessel	Present		3G	Expression is in large blood vessels in the liver.
TS24: liver vasculature	Present		3G	Expression is in large blood vessels in the liver.
TS24: liver parenchyma	Absent		3G

Structure	Level	Pattern	Image	Note
TS26: pubis	Strong	Regionally restricted	3H	Expression is on the outer part.
TS26: blood vessel	Present		3H	Expression is in penile blood vessels.
TS26: detrusor muscle of bladder	Absent		3H
TS26: seminal vesicle	Absent		3H
TS26: ureter	Absent		3H
TS26: urethra of male	Absent		3H
TS26: urothelium of bladder	Absent		3H

Structure	Level	Pattern	Image	Note
TS24: early nephron	Present	Regionally restricted	3I	Expression is in round structures developing into glomerular structures.
TS24: arterial blood vessel	Present	Regionally restricted	3I	Expression is in large arteries in the kidney.
TS24: metanephros	Present	Regionally restricted	3I	Expression is in round structures developing into glomerular structures and in large arteries and is absent in most parts of the kidney parenchyma.
TS24: collecting duct	Absent		3I
TS24: ureter	Absent		3I
TS24: urethra	Absent		3I

Structure	Level	Pattern	Image	Note
TS26: glomerular tuft	Present		3J
TS26: renal interstitium	Moderate	Regionally restricted	3J	Expression is stronger in aggregates of smaller caliber vessels.
TS26: metanephros epithelium	Absent		3J

Structure	Level	Pattern	Image	Note
TS26: submandibular gland mesenchyme	Present	Regionally restricted	3K	There is some vascular and mesenchymal expression.
TS26: submandibular gland epithelium	Absent		3K

Structure	Level	Pattern	Image	Note
TS24: thymus	Absent		3L

Structure	Level	Pattern	Image	Note
TS24: blood vessel	Present	Regionally restricted	3M	Expression is in small vessels in the adrenal gland.
TS24: adrenal cortex	Absent		3M
TS24: adrenal medulla	Absent		3M

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Strong	Regionally restricted	4A	Expression is in layer VII large cortical neurons and in the continuous layer of neuroepithelial cells lining the ventricle.
TS26: brain blood vessel	Absent		4A
TS26: cerebral cortex subventricular zone	Absent		4A

Structure	Level	Pattern	Image	Note
TS27: brain blood vessel	Strong	Regionally restricted	4B	Expression is in small vessels.

Structure	Level	Pattern	Image	Note
TS27: neocortex layer	Present	Regionally restricted	4C	Expression is in layer VII neurons.
TS27: main olfactory bulb	Present		4C
TS27: brain blood vessel	Trace		4C

Structure	Level	Pattern	Image	Note
TS20: mystacial pad	Moderate	Regionally restricted	5A	Expression is diffuse in the subcutaneous mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: mystacial pad	Strong	Regionally restricted	5B	Expression is in the mesenchyme surrounding the whisker hair follicles.
TS22: epidermis	Absent		5B

Structure	Level	Pattern	Image	Note
TS27: dermis connective tissue	Weak	Diffuse	5C	Expression is diffuse in the subcutaneous mesenchyme and is moderate around the hair follicles.

Structure	Level	Pattern	Image	Note
TS27: mystacial pad	Strong	Regionally restricted	5D	Expression is in several layers surrounding the whisker hair follicles and just below the skin epithelium. Weak in mesenchyme far removed from the follicles.

Structure	Level	Pattern	Image	Note
TS26: lip	Present	Regionally restricted	5E	Expression is in the perioral subcutaneous mesenchyme and ends just at the border of the inner lip.
TS26: oral region mesenchyme	Present	Regionally restricted	5E	Expression is in the perioral subcutaneous mesenchyme and ends just at the border of the inner lip.
TS26: tongue	Present	Regionally restricted	5E	Expression is in the dorsal region and is absent in the apical part.

Structure	Level	Pattern	Image	Note
TS26: eye	Moderate		5F	Expression is in small vessels lining the outer retinal surface.
TS26: hyaloid vascular plexus	Moderate	Regionally restricted	5F	Expression is in hyaloid vessels lining the posterior lens surface.
TS26: sclera	Moderate	Regionally restricted	5F	Expression is in the mesenchyme.
TS26: iris	Absent		5F
TS26: lens	Absent		5F
TS26: retina	Absent		5F

Structure	Level	Pattern	Image	Note
TS27: sphenoid bone	Moderate	Regionally restricted	5G	Expression is in the mesenchymal lining of the petrosal bone.
TS27: organ of Corti	Moderate		5G

Structure	Level	Pattern	Image	Note
TS27: spiral limbus	Present	Regionally restricted	5H	Expression is in the mesenchyme and the vestibular limbus lip.
TS27: tympanic membrane	Present	Regionally restricted	5H	Expression is in tympanal perithelial cells.
TS27: stria vascularis	Absent		5H
TS27: vestibular membrane	Absent		5H

Structure	Level	Pattern	Image	Note
TS26: tooth mesenchyme	Strong	Regionally restricted	5I	Expression is at the surface of the primary tooth bud meeting with the inner dental epithelium and is absent around the outer dental epithelium.
TS26: oral epithelium	Weak	Regionally restricted	5I	Expression is in early invaginating epithelium.
TS26: oral region mesenchyme	Weak		5I

Structure	Level	Pattern	Image	Note
TS27: molar dental papilla	Moderate	Regionally restricted	5J	Expression is in the subodontoblast mesenchyme.
TS27: stellate reticulum	Moderate		5J
TS27: molar epithelium	Present	Regionally restricted	5J	There is focal expression of the bicuspid epithelium.

Structure	Level	Pattern	Image	Note
TS27: lower jaw molar enamel organ	Strong		5K
TS27: inner enamel epithelium	Strong	Spotted	5K
TS27: outer enamel epithelium	Strong	Spotted	5K

Structure	Level	Pattern	Image	Note
TS22: tooth	Present			Expression is in tooth formation from E14-P3.

Structure	Level	Pattern	Image	Note
TS27: perichondrium	Strong	Regionally restricted		Expression is on the nasal bones.
TS27: connective tissue	Strong	Regionally restricted		Expression is in connective tissue lining conchal cartilage.
TS27: turbinate bone	Strong	Regionally restricted		Expression is in connective tissue lining conchal cartilage.

Structure	Level	Pattern	Image	Note
TS28: adrenal gland	Present
TS28: blood vessel endothelium	Present
TS28: lung vascular element	Present