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Gene Expression Data
Assay Details
Assay
Reference: J:227628 Sarkar MK, et al., An Xist-activating antisense RNA required for X-chromosome inactivation. Nat Commun. 2015;6:8564
Assay type: RNA in situ
MGI Accession ID: MGI:6719343
Gene symbol: Xist
Gene name: inactive X specific transcripts
Probe: XistAR probe1
Probe preparation: labelled with Cy3 RNA
Results
Specimen 2a: embryonic day 3.5 (more )
Note: Triple labeled: red - XistAR (Xist Activating RNA); green - Xist (assay MGI:6719342); white - Atrx (assay MGI:6719344).
Structure Level Pattern Image Note
TS4: blastocyst Present Regionally restricted 2a Expression in substantial percentage (~28%) of nuclei adjacent to or overlapping with Xist-expressing coat.

Specimen 2a inset: embryonic day 3.5 (more )
Note: Triple labeled: red - XistAR (Xist Activating RNA); green - Xist (assay MGI:6719342); white - Atrx (assay MGI:6719344).
Structure Level Pattern Image Note
TS4: blastocyst Present Regionally restricted 2a inset Expression in substantial percentage (~28%) of nuclei adjacent to or overlapping with Xist-expressing coat.

Specimen 2b: embryonic day 6.5 (more )
Note: Triple labeled: red - XistAR (Xist Activating RNA); green - Xist (assay MGI:6719342); white - Atrx (assay MGI:6719344).
Structure Level Pattern Image Note
TS8: extraembryonic ectoderm Present Regionally restricted 2b Expression from the inactive X in ~40% of the nuclei.

Specimen 2c: embryonic day 6.5 (more )
Note: Triple labeled: red - XistAR (Xist Activating RNA); green - Xist (assay MGI:6719342); white - Atrx (assay MGI:6719344).
Structure Level Pattern Image Note
TS8: epiblast Present Regionally restricted 2c Expression in ~48% of randomly inactivated epiblast cells.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory