Antibody:	anti-Myo6 (25-6791)
Detection system:	Secondary antibody coupled to Alexa Fluor

Structure	Level	Pattern	Image	Note
TS27: cochlea	Present	Regionally restricted	1c merge , 1c MYO6	Expression is in the cytoplasm of the inner and outer hair cells.

Structure	Level	Pattern	Image	Note
TS27: cochlea	Present	Regionally restricted	1e MYO6 , 1e merge	Expression is in the cytoplasm of the inner and outer hair cells.

Structure	Level	Pattern	Image	Note
TS22: cochlea	Absent		2g MYO6 , 2g Merge	No hair cells were seen in the cochlea at this age.

Structure	Level	Pattern	Image	Note
TS23: cochlea	Present	Regionally restricted	2h Merge , 2h MYO6	Expression is seen in inner and outer hair cells.

Structure	Level	Pattern	Image	Note
TS27: cochlea	Present	Regionally restricted	2i Merge , 2i MYO6	Expression is seen in inner and outer hair cells.

Structure	Level	Pattern	Image	Note
TS21: utricle epithelium	Present	Regionally restricted	5b WT E13.5 upper left	Expression is seen in the hair cells.

Structure	Level	Pattern	Image	Note
TS21: saccule epithelium	Present	Regionally restricted	5b WT E13.5 upper right	Expression is seen in the hair cells.

Structure	Level	Pattern	Image	Note
TS22: superior semicircular canal epithelium	Present	Regionally restricted	5b WT E14.5 lower left	Expression is seen in the hair cells of the anterior crista.
TS22: lateral semicircular canal epithelium	Present	Regionally restricted	5b WT E14.5 lower left	Expression is seen in the hair cells of the lateral crista.

Structure	Level	Pattern	Image	Note
TS22: cochlear duct	Absent		5b WT E14.5 lower right	Hair cells have not differentiated at this age.

Structure	Level	Pattern	Image	Note
TS21: utricle epithelium	Present	Regionally restricted	5b hom E13.5 upper left	Expression is seen in the hair cells; no differences in expression were observed between wild type and the mutants.

Structure	Level	Pattern	Image	Note
TS21: saccule epithelium	Present	Regionally restricted	5b hom E13.5 lower left	Expression is seen in the hair cells; no differences in expression were observed between wild type and the mutants.

Structure	Level	Pattern	Image	Note
TS21: posterior semicircular canal epithelium	Present	Regionally restricted	5b hom E13.5 upper right	Expression is seen in the hair cells of the posterior crista; no differences in expression were observed between wild type and the mutants.

Structure	Level	Pattern	Image	Note
TS21: cochlear duct	Absent		5b hom E13.5 lower right	As in wild type, hair cells have not differentiated at this age.

Structure	Level	Pattern	Image	Note
TS22: lateral semicircular canal epithelium	Present	Regionally restricted	5b hom E14.5 upper left	Expression is seen in the hair cells of the lateral crista; no differences in expression were observed between wild type and the mutants.
TS22: superior semicircular canal epithelium	Present	Regionally restricted	5b hom E14.5 upper left	Expression is seen in the hair cells of the anterior crista; no differences in expression were observed between wild type and the mutants.

Structure	Level	Pattern	Image	Note
TS22: utricle epithelium	Present	Regionally restricted	5b hom E14.5 lower left	Expression is seen in the hair cells; no differences in expression were observed between wild type and the mutants.

Structure	Level	Pattern	Image	Note
TS22: saccule epithelium	Present	Regionally restricted	5b hom E14.5 upper right	Expression is seen in the hair cells; no differences in expression were observed between wild type and the mutants.

Structure	Level	Pattern	Image	Note
TS22: cochlear duct	Absent		5b hom E14.5 lower right	As in wild type, hair cells have not differentiated at this age.

Structure	Level	Pattern	Image	Note
TS23: cochlear duct epithelium	Present	Regionally restricted	5b WT E15.5 upper	Expression is seen in the hair cells.

Structure	Level	Pattern	Image	Note
TS25: utricle epithelium	Present	Regionally restricted	5b WT E17.5 left	Expression is seen in the hair cells.

Structure	Level	Pattern	Image	Note
TS25: cochlear duct epithelium	Present	Regionally restricted	5b WT E17.5 right	Expression is seen in the hair cells.

Structure	Level	Pattern	Image	Note
TS23: lateral semicircular canal epithelium	Present	Regionally restricted	5b hom E15.5 upper left	Expression is seen in the hair cells of the lateral crista; no differences in expression were observed between wild type and the mutants.
TS23: superior semicircular canal epithelium	Present	Regionally restricted	5b hom E15.5 upper left	Expression is seen in the hair cells of the anterior crista; no differences in expression were observed between wild type and the mutants.

Structure	Level	Pattern	Image	Note
TS23: posterior semicircular canal epithelium	Present	Regionally restricted	5b hom E15.5 upper right	Expression is seen in the hair cells of the posterior crista; no differences in expression were observed between wild type and the mutants.

Structure	Level	Pattern	Image	Note
TS23: utricle epithelium	Present	Regionally restricted	5b hom E15.5 lower left	Expression is seen in the hair cells; no differences in expression were observed between wild type and the mutants.

Structure	Level	Pattern	Image	Note
TS23: cochlear duct epithelium	Present	Regionally restricted	5b hom E15.5 lower right	Expression is seen in the hair cells; no differences in expression were observed between wild type and the mutants.

Structure	Level	Pattern	Image	Note
TS25: lateral semicircular canal epithelium	Present	Regionally restricted	5b hom E17.5 upper left	Expression is seen in the hair cells of the lateral crista; no differences in expression were observed between wild type and the mutants.
TS25: superior semicircular canal epithelium	Present	Regionally restricted	5b hom E17.5 upper left	Expression is seen in the hair cells of the anterior crista; no differences in expression were observed between wild type and the mutants.

Structure	Level	Pattern	Image	Note
TS25: posterior semicircular canal epithelium	Present	Regionally restricted	5b hom E17.5 upper right	Expression is seen in the hair cells of the posterior crista; no differences in expression were observed between wild type and the mutants.

Structure	Level	Pattern	Image	Note
TS25: utricle epithelium	Present	Regionally restricted	5b hom E17.5 lower left	Expression is seen in the hair cells; no differences in expression were observed between wild type and the mutants.

Structure	Level	Pattern	Image	Note
TS25: saccule epithelium	Present	Regionally restricted	5b hom E17.5 lower center	Expression is seen in the hair cells; no differences in expression were observed between wild type and the mutants.

Structure	Level	Pattern	Image	Note
TS25: cochlear duct epithelium	Present	Regionally restricted	5b hom E17.5 lower right	Expression is seen in the hair cells; no differences in expression were observed between wild type and the mutants.

Structure	Level	Pattern	Image	Note
TS28: cochlear duct epithelium	Present	Regionally restricted	6a upper left , 6a Apex Merge , 6a Apex MYO6	Expression is seen in the inner and outer cells of the apical cochlear duct.

Structure	Level	Pattern	Image	Note
TS28: cochlear duct epithelium	Present	Regionally restricted	6a Middle MYO6 , 6a Middle Merge , 6a upper center	Expression is seen in the inner and outer cells of the middle cochlear duct.

Structure	Level	Pattern	Image	Note
TS28: cochlear duct epithelium	Present	Regionally restricted	6a Basal MYO6 , 6a Basal Merge , 6a upper right	Expression is seen in the inner and outer cells of the basal cochlear duct.

Structure	Level	Pattern	Image	Note
TS28: cochlear duct epithelium	Present	Regionally restricted	6b Apex MYO6 , 6b upper left , 6b Apex Merge	Expression is seen in the inner and outer cells of the apical cochlear duct; the number and organization of these cells is the same in the mutant as in wild type.

Structure	Level	Pattern	Image	Note
TS28: cochlear duct epithelium	Present	Regionally restricted	6b Middle MYO6 , 6b Middle Merge , 6b upper center	Expression is seen in the inner and outer cells of the middle cochlear duct; the number and organization of these cells is the same in the mutant as in wild type.

Structure	Level	Pattern	Image	Note
TS28: cochlear duct epithelium	Present	Regionally restricted	6b upper right , 6b Basal MYO6 , 6b Basal Merge	Expression is seen in the inner and outer cells of the basal cochlear duct; the number and organization of these cells is the same in the mutant as in wild type.