H2ax Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:231231 Abby E, et al., Implementation of meiosis prophase I programme requires a conserved retinoid-independent stabilizer of meiotic transcripts. Nat Commun. 2016;7:10324
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6715676
Gene symbol:	H2ax
Gene name:	H2A.X variant histone

Antibody:	anti-phosphoH2AX (#05-636; JBW301)
Detection system:	Secondary antibody coupled to horseradish peroxidase

Results

4c Meioc+/+ gamma-H2AX ©

Specimen 4c +/+: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: meiotic germ cell of ovary	Present		4c Meioc+/+ gamma-H2AX

4c Meioc-/- gamma-H2AX ©

Specimen 4c -/-: embryonic day 15.5; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS23: meiotic germ cell of ovary	Weak		4c Meioc-/- gamma-H2AX	The meiotic marker was poorly detected, although germ cell density appeared unaltered.

4e Meioc+/+ Vasa/gamma-H2AX ©

Specimen 4e +/+ VASA/g-H2AX: embryonic day 18.5 (more

)
Note: Double labeled: brown - H2ax; magenta - Ddx4 (assay MGI:6695761).

Structure	Level	Pattern	Image	Note
TS26: meiotic germ cell of ovary	Weak		4e Meioc+/+ Vasa/gamma-H2AX	Expression of H2ax was progressively lost during later fetal life in wild type.

4e Meioc-/- Vasa/gamma-H2AX ©

Specimen 4e -/- VASA/g-H2AX: embryonic day 18.5; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Double labeled: brown - H2ax; magenta - Ddx4 (assay MGI:6695761).

Structure	Level	Pattern	Image	Note
TS26: meiotic germ cell of ovary	Present		4e Meioc-/- Vasa/gamma-H2AX	An abundance of staining for H2ax was detected in the mutant.

5a 18.5dpc Meioc+/+ gamma-H2AX ©

Specimen 5a g-H2AX 18.5-dpc +/+: embryonic day 18.5 (more

)

Structure	Level	Pattern	Image	Note
TS26: germ cell of testis	Absent		5a 18.5dpc Meioc+/+ gamma-H2AX

5a 18.5dpc Meioc-/- gamma-H2AX ©

Specimen 5a g-H2AX 18.5-dpc -/-: embryonic day 18.5; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS26: germ cell of testis	Absent		5a 18.5dpc Meioc-/- gamma-H2AX

5a 6dpp Meioc+/+ gamma-H2AX ©

Specimen 5a g-H2AX 6-dpp +/+: postnatal day 6 (more

)

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Absent		5a 6dpp Meioc+/+ gamma-H2AX

5a 6dpp Meioc-/- gamma-H2AX ©

Specimen 5a g-H2AX 6-dpp -/-: postnatal day 6; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Absent		5a 6dpp Meioc-/- gamma-H2AX

5a 8dpp Meioc+/+ gamma-H2AX ©

Specimen 5a g-H2AX 8-dpp +/+: postnatal day 8 (more

)

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		5a 8dpp Meioc+/+ gamma-H2AX	Meiosis initiated at 8 dpp.

5a 8dpp Meioc-/- gamma-H2AX ©

Specimen 5a g-H2AX 8-dpp -/-: postnatal day 8; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Weak		5a 8dpp Meioc-/- gamma-H2AX	At 8 dpp H2ax appeared to be globally weaker in the mutant than in wild type testes.

5a 10dpp Meioc+/+ gamma-H2AX ©

Specimen 5a g-H2AX 10-dpp +/+: postnatal day 10 (more

)

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		5a 10dpp Meioc+/+ gamma-H2AX

5a 10dpp Meioc-/- gamma-H2AX ©

Specimen 5a g-H2AX 10-dpp -/-: postnatal day 10; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		5a 10dpp Meioc-/- gamma-H2AX	A progressive accumulation of H2ax-positive cells was noted in later stages.

5a 16dpp Meioc+/+ gamma-H2AX ©

Specimen 5a g-H2AX 16-dpp +/+: postnatal day 16 (more

)

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		5a 16dpp Meioc+/+ gamma-H2AX

5a 16dpp Meioc-/- gamma-H2AX ©

Specimen 5a g-H2AX 16-dpp -/-: postnatal day 16; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		5a 16dpp Meioc-/- gamma-H2AX	A progressive accumulation of H2ax-positive cells was noted in later stages.

5a 20dpp Meioc+/+ gamma-H2AX ©

Specimen 5a g-H2AX 20-dpp +/+: postnatal day 20 (more

)

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		5a 20dpp Meioc+/+ gamma-H2AX

Specimen 5a g-H2AX 20-dpp -/-: postnatal day 20; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		5a 20dpp Meioc-/- gamma-H2AX	A progressive accumulation of H2ax-positive cells was noted in later stages.

Specimen 5a g-H2AX Adult +/+: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		5a Adult Meioc+/+ gamma-H2AX

Specimen 5a g-H2AX Adult -/-: postnatal adult; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		5a Adult Meioc-/- gamma-H2AX	A progressive accumulation of H2ax-positive cells was noted in later stages.

7a right ©

Specimen 7a: postnatal day 16; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: testis	Present		7a right

S4e +/+ ©

Specimen S4e +/+: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S4e +/+

S4e -/- ©

Specimen S4e -/-: postnatal adult; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S4e -/-	All spermatocytes were positive for H2ax in the mutant testis.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 05/07/2024 MGI 6.23