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Gene Expression Data
Assay Details
Assay
Reference: J:263030 Hu J, et al., WDR1-regulated actin dynamics is required for outflow tract and right ventricle development. Dev Biol. 2018 Jun 15;438(2):124-137
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6715546
Gene symbol: Tnnt2
Gene name: troponin T2, cardiac
Results
Specimen S3F: embryonic day 9.5; Tg(Mef2c-cre)2Blk/0, Wdr1tm1.1Zzy/Wdr1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS15: outflow tract Present S3F
TS15: primitive ventricle Present S3F
Specimen S3J: embryonic day 9.5; Tg(Mef2c-cre)2Blk/0, Wdr1tm1.1Zzy/Wdr1tm1.1Zzy (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS15: outflow tract Present S3J Authors report no expansion of expression domains compared with control.
TS15: primitive ventricle Present S3J Authors report no expansion of expression domains compared with control.
Specimen E10.5 CTL not shown: embryonic day 10.5; Tg(Mef2c-cre)2Blk/0, Wdr1tm1.1Zzy/Wdr1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: outflow tract Present Number of cardiomyocytes in outflow trac and right ventricle were similar betwen control and mutant embryos at E9.5 and E10.5.
TS17: primitive ventricle Present Number of cardiomyocytes in outflow trac and right ventricle were similar betwen control and mutant embryos at E9.5 and E10.5.
Specimen E10.5 shfKO not shown: embryonic day 10.5; Tg(Mef2c-cre)2Blk/0, Wdr1tm1.1Zzy/Wdr1tm1.1Zzy (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: outflow tract Present Number of cardiomyocytes in outflow trac and right ventricle were similar betwen control and mutant embryos at E9.5 and E10.5.
TS17: primitive ventricle Present Number of cardiomyocytes in outflow trac and right ventricle were similar betwen control and mutant embryos at E9.5 and E10.5.
Specimen 6A: embryonic day 9.5; Tg(Mef2c-cre)2Blk/0, Wdr1tm1.1Zzy/Wdr1+ (more )
Note: Conditional mutant. Triple labeled: green - Tnnt2; gray - Ctnnb1 (assay MGI:6715553); red - phalloidin.
Structure Level Pattern Image Note
TS15: outflow tract Present Regionally restricted 6A Authors report proximal outflow tract composed of compact and tidily aligned monolayed/bilayed cardiomyocytes.
Specimen 6B: embryonic day 9.5; Tg(Mef2c-cre)2Blk/0, Wdr1tm1.1Zzy/Wdr1tm1.1Zzy (more )
Note: Conditional mutant. Triple labeled: green - Tnnt2; gray - Ctnnb1 (assay MGI:6715553); red - phalloidin.
Structure Level Pattern Image Note
TS15: outflow tract Present Regionally restricted 6B Organization of cardiac layers disrupted: cardiomyocytes loosely and disorderly arranged, and myocardium multilayered.
Specimen S5H: embryonic day 9.5; Tg(Mef2c-cre)2Blk/0, Wdr1tm1.1Zzy/Wdr1+ (more )
Note: Conditional mutant. Double labeled: red - Tnnt2; green - Emcn (assay MGI:6715554).
Structure Level Pattern Image Note
TS15: primitive ventricle Present S5H
Specimen S5J: embryonic day 9.5; Tg(Mef2c-cre)2Blk/0, Wdr1tm1.1Zzy/Wdr1tm1.1Zzy (more )
Note: Conditional mutant. Double labeled: red - Tnnt2; green - Emcn (assay MGI:6715554).
Structure Level Pattern Image Note
TS15: primitive ventricle Present Regionally restricted S5J No apparent abnormalities in mutant; endothelial cells tidily lining well-developed trabecula and myocardium.
Specimen 7A/C: embryonic day 9.5; Tg(Mef2c-cre)2Blk/0, Wdr1tm1.1Zzy/Wdr1+ (more )
Note: Conditional mutant. Double labeled: green - Tnnt2; red - phalloidin.
Structure Level Pattern Image Note
TS15: cardiac muscle tissue Present Regionally restricted 7C , 7A Inner cellular layers were longitudinal striated cardiac myofibrils; expression aligned in striation. Outer monlolayer/bilayer complosed of closely connected cardioyocytes.
Specimen 7D/F: embryonic day 9.5; Tg(Mef2c-cre)2Blk/0, Wdr1tm1.1Zzy/Wdr1tm1.1Zzy (more )
Note: Conditional mutant. Double labeled: green - Tnnt2; red - phalloidin.
Structure Level Pattern Image Note
TS15: cardiac muscle tissue Present Regionally restricted 7D , 7F Cardiomyocytes loosely and disorderly organized into multilayered myocardium. Number of striated longitudinal myofibrils decreased. Striated pattern of myofibrils lost in regions of F-actin accumulation.
Specimen S6A/C: embryonic day 9.5; Nkx2-5tm1(cre)Rjs/Nkx2-5+, Wdr1tm1.1Zzy/Wdr1+ (more )
Note: Conditional mutant. Double labeled: green - Tnnt2; red - phalloidin.
Structure Level Pattern Image Note
TS15: primitive ventricle Present S6A , S6C
Specimen S6D/F: embryonic day 9.5; Nkx2-5tm1(cre)Rjs/Nkx2-5+, Wdr1tm1.1Zzy/Wdr1+ (more )
Note: Conditional mutant. Double labeled: green - Tnnt2; red - phalloidin.
Structure Level Pattern Image Note
TS15: primitive ventricle Present S6D , S6F
Specimen S6G/I: embryonic day 9.5; Nkx2-5tm1(cre)Rjs/Nkx2-5+, Wdr1tm1.1Zzy/Wdr1tm1.1Zzy (more )
Note: Conditional mutant. Double labeled: green - Tnnt2; red - phalloidin.
Structure Level Pattern Image Note
TS15: primitive ventricle Present Regionally restricted S6G , S6I Abundant expression, striated pattern lost in regions of F-actin accumulation. Disruption of sarcomeric structure in both left and right ventricles.
Specimen S6J/L: embryonic day 9.5; Nkx2-5tm1(cre)Rjs/Nkx2-5+, Wdr1tm1.1Zzy/Wdr1tm1.1Zzy (more )
Note: Conditional mutant. Double labeled: green - Tnnt2; red - phalloidin.
Structure Level Pattern Image Note
TS15: primitive ventricle Present Regionally restricted S6L , S6J Abundant expression, striated pattern lost in regions of F-actin accumulation. Disruption of sarcomeric structure in both left and right ventricles.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
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