Gene Expression Data
Assay Details
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Assay
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Reference:
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J:301247
Probst S, et al., Spatiotemporal sequence of mesoderm and endoderm lineage segregation during mouse gastrulation. Development. 2021 Jan 7;148(1):dev193789
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6713515
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Gene symbol:
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Mesp1
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Gene name:
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mesoderm posterior 1
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Reporter:
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Venus
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Direct Detection
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Results
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Specimen
2D:
(close  )
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Genetic Background:
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involves: 129S/SvEv * NMRI
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Mutant Allele(s):
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Mesp1tm1.1Sjar/Mesp1+
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS9: embryo ectoderm |
Present |
Regionally restricted |
2D
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Expressing cells appear as early as E6.5 in proximal epiblast during primitive streak formation.
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Specimen
2E:
(close )
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Genetic Background:
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involves: 129S/SvEv * NMRI
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Mutant Allele(s):
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Mesp1tm1.1Sjar/Mesp1+
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS9: embryo ectoderm |
Present |
Regionally restricted |
2E
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Expression in early gastrulating cells in proximal epiblast.
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Specimen
2E inset:
(close )
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Genetic Background:
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involves: 129S/SvEv * NMRI
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Mutant Allele(s):
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Mesp1tm1.1Sjar/Mesp1+
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Age:
|
embryonic day 6.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS9: primitive streak |
Present |
Regionally restricted |
2E zoom
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Expresion in most cells leaving the early proximal primitive streak, identifying these cells as mesoderm progenitors.
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Specimen
2F:
(close )
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Genetic Background:
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involves: 129S/SvEv * NMRI
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Mutant Allele(s):
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Mesp1tm1.1Sjar/Mesp1+
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|
Age:
|
embryonic day 6.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS9: embryo ectoderm |
Present |
Regionally restricted |
2F
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No expressing cells in distal portions of epiblast.
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Specimen
2G:
(close )
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Genetic Background:
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involves: 129S/SvEv * NMRI
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Mutant Allele(s):
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Mesp1tm1.1Sjar/Mesp1+
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Age:
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embryonic day 6.75
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: embryo mesoderm |
Present |
Regionally restricted |
2G
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Expressing cells rapidly migrate proximally towards the extraembryonic mesoderm and anteriorly toward anterior mesoderm.
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Specimen
2H:
(close )
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Genetic Background:
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involves: 129S/SvEv * NMRI
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Mutant Allele(s):
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Mesp1tm1.1Sjar/Mesp1+
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Age:
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embryonic day 6.75
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: primitive streak |
Present |
Regionally restricted |
2H
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Expressing cells in the epithelial primitive streak - indicating mesoderm specification takes place before cells delaminate from epiblast.
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Specimen
2H inset:
(close )
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Genetic Background:
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involves: 129S/SvEv * NMRI
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Mutant Allele(s):
|
Mesp1tm1.1Sjar/Mesp1+
|
|
Age:
|
embryonic day 6.75
|
|
Sex:
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Not Specified
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Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS10: primitive streak |
Present |
Regionally restricted |
2H zoom
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Expressing cells in epithelial portion of primitive streak.
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Specimen
2I:
embryonic day 7.0; Mesp1tm1.1Sjar/Mesp1 +
(more  )
Note: Triple labeled: green - Mesp1 reporter; red - Eomes (assay MGI:6713521); blue - Dapi.
Specimen
2I:
(close )
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Genetic Background:
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involves: 129S/SvEv * NMRI
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Mutant Allele(s):
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Mesp1tm1.1Sjar/Mesp1+
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Age:
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embryonic day 7.0
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Sex:
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Not Specified
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Type:
|
optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Triple labeled: green - Mesp1 reporter; red - Eomes (assay MGI:6713521); blue - Dapi.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: embryo mesoderm |
Present |
Regionally restricted |
2I
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Expressing cells constituting a major population of cells within the mesoderm layer - intermingled with non-expressing cells.
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Specimen
2J:
(close )
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Genetic Background:
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involves: 129S/SvEv * NMRI
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Mutant Allele(s):
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Mesp1tm1.1Sjar/Mesp1+
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Age:
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embryonic day 7.25
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo mesoderm |
Present |
Regionally restricted |
2J
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Expression in mesodermal wings that have migrated far anteriorly.
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Specimen
2K:
(close )
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Genetic Background:
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involves: 129S/SvEv * NMRI
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Mutant Allele(s):
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Mesp1tm1.1Sjar/Mesp1+
|
|
Age:
|
embryonic day 7.25
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Sex:
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Not Specified
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Type:
|
optical section
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|
Fixation:
|
4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: primitive streak |
Present |
Regionally restricted |
2K
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Expressing cells still emerging from proximal primtive streak.
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Specimen
2L:
(close )
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Genetic Background:
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involves: 129S/SvEv * NMRI
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Mutant Allele(s):
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Mesp1tm1.1Sjar/Mesp1+
|
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Age:
|
embryonic day 7.25
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Sex:
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Not Specified
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Type:
|
optical section
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|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS11: primitive streak |
Present |
Regionally restricted |
2L
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No expression in more distal primitive streak.
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Specimen
2L inset:
(close )
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Genetic Background:
|
involves: 129S/SvEv * NMRI
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Mutant Allele(s):
|
Mesp1tm1.1Sjar/Mesp1+
|
|
Age:
|
embryonic day 7.25
|
|
Sex:
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Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo mesoderm |
Present |
Regionally restricted |
2L zoom
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Expressing cells intermigled with non-expressing cells mostly toward distal regions.
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Specimen
3A:
embryonic day 6.5; Mesp1tm1.1Sjar/Mesp1 +
(more )
Note: Triple labeled: green - Mesp1 reporter; red - Foxa2 (assay MGI:6713524); blue - Dapi.
Specimen
3A:
(close )
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Genetic Background:
|
involves: 129S/SvEv * NMRI
|
|
Mutant Allele(s):
|
Mesp1tm1.1Sjar/Mesp1+
|
|
Age:
|
embryonic day 6.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Triple labeled: green - Mesp1 reporter; red - Foxa2 (assay MGI:6713524); blue - Dapi.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS9: embryo ectoderm |
Present |
Regionally restricted |
3A
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Expressing cells in proximal epiblast.
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| TS9: embryo mesoderm |
Present |
Regionally restricted |
3A
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Expression in anterior mesoderm cells, some of which have delaminated from the primitive streak.
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Specimen
3B:
embryonic day 6.75; Mesp1tm1.1Sjar/Mesp1 +
(more )
Note: Triple labeled: green - Mesp1 reporter; red - Foxa2 (assay MGI:6713524); blue - Dapi.
Specimen
3B:
(close )
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Genetic Background:
|
involves: 129S/SvEv * NMRI
|
|
Mutant Allele(s):
|
Mesp1tm1.1Sjar/Mesp1+
|
|
Age:
|
embryonic day 6.75
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Triple labeled: green - Mesp1 reporter; red - Foxa2 (assay MGI:6713524); blue - Dapi.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS10: embryo mesoderm |
Present |
Regionally restricted |
3B
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Expressing cells in proximal anterior mesoderm.
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Specimen
3C:
embryonic day 6.5; Mesp1tm1.1Sjar/Mesp1 +
(more )
Note: Triple labeled: green - Mesp1 reporter; red - Foxa2 (assay MGI:6713524); blue - Dapi.
Specimen
3C:
(close )
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Genetic Background:
|
involves: 129S/SvEv * NMRI
|
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Mutant Allele(s):
|
Mesp1tm1.1Sjar/Mesp1+
|
|
Age:
|
embryonic day 6.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Triple labeled: green - Mesp1 reporter; red - Foxa2 (assay MGI:6713524); blue - Dapi.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS9: embryo mesoderm |
Present |
Regionally restricted |
3C
|
Expression in anterior mesoderm cells, some of which have delaminated from the primitive streak.
|
Specimen
3D:
embryonic day 6.5; Mesp1tm1.1Sjar/Mesp1 +
(more )
Note: Triple labeled: green - Mesp1 reporter; red - Foxa2 (assay MGI:6713524); blue - Dapi.
Specimen
3D:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * NMRI
|
|
Mutant Allele(s):
|
Mesp1tm1.1Sjar/Mesp1+
|
|
Age:
|
embryonic day 6.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Triple labeled: green - Mesp1 reporter; red - Foxa2 (assay MGI:6713524); blue - Dapi.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS9: embryo mesoderm |
Present |
Regionally restricted |
3D
|
Expression in anterior mesoderm cells, some of which have delaminated from the primitive streak.
|
Specimen
3E:
embryonic day 6.5; Mesp1tm1.1Sjar/Mesp1 +
(more )
Note: Triple labeled: green - Mesp1 reporter; red - Foxa2 (assay MGI:6713524); blue - Dapi.
Specimen
3E:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * NMRI
|
|
Mutant Allele(s):
|
Mesp1tm1.1Sjar/Mesp1+
|
|
Age:
|
embryonic day 6.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Triple labeled: green - Mesp1 reporter; red - Foxa2 (assay MGI:6713524); blue - Dapi.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS9: embryo mesoderm |
Present |
Regionally restricted |
3E
|
Expression in anterior mesoderm cells, some of which have delaminated from the primitive streak.
|
Specimen
3F:
embryonic day 6.5; Mesp1tm1.1Sjar/Mesp1 +
(more )
Note: Triple labeled: green - Mesp1 reporter; red - Foxa2 (assay MGI:6713524); blue - Dapi.
Specimen
3F:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * NMRI
|
|
Mutant Allele(s):
|
Mesp1tm1.1Sjar/Mesp1+
|
|
Age:
|
embryonic day 6.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Triple labeled: green - Mesp1 reporter; red - Foxa2 (assay MGI:6713524); blue - Dapi.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS9: embryo mesoderm |
Present |
Regionally restricted |
3F
|
Expression in anterior mesoderm cells, some of which have delaminated from the primitive streak.
|
Specimen
3G:
embryonic day 7.0; Mesp1tm1.1Sjar/Mesp1 +
(more )
Note: Triple labeled: green - Mesp1 reporter; red - Foxa2 (assay MGI:6713524); blue - Dapi.
Specimen
3G:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * NMRI
|
|
Mutant Allele(s):
|
Mesp1tm1.1Sjar/Mesp1+
|
|
Age:
|
embryonic day 7.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Triple labeled: green - Mesp1 reporter; red - Foxa2 (assay MGI:6713524); blue - Dapi.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS10: embryo mesoderm |
Present |
Regionally restricted |
3G
|
In more proximal sections, expression in primitive streak and cells of mesoderm layer.
|
| TS10: primitive streak |
Present |
Regionally restricted |
3G
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In more proximal sections, expression in primitive streak and cells of mesoderm layer.
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Specimen
3H:
embryonic day 7.0; Mesp1tm1.1Sjar/Mesp1 +
(more )
Note: Triple labeled: green - Mesp1 reporter; red - Foxa2 (assay MGI:6713524); blue - Dapi.
Specimen
3H:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * NMRI
|
|
Mutant Allele(s):
|
Mesp1tm1.1Sjar/Mesp1+
|
|
Age:
|
embryonic day 7.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Triple labeled: green - Mesp1 reporter; red - Foxa2 (assay MGI:6713524); blue - Dapi.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS10: primitive streak |
Present |
Regionally restricted |
3H
|
Intermediate zone of intermixed Mesp1-expressing and Foxa2-expressing cells. Few double-labeled cells are present.
|
Specimen
3I:
embryonic day 7.0; Mesp1tm1.1Sjar/Mesp1 +
(more )
Note: Triple labeled: green - Mesp1 reporter; red - Foxa2 (assay MGI:6713524); blue - Dapi.
Specimen
3I:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * NMRI
|
|
Mutant Allele(s):
|
Mesp1tm1.1Sjar/Mesp1+
|
|
Age:
|
embryonic day 7.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Triple labeled: green - Mesp1 reporter; red - Foxa2 (assay MGI:6713524); blue - Dapi.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS10: primitive streak |
Present |
Regionally restricted |
3I
|
Intermediate zone of intermixed Mesp1-expressing and Foxa2-expressing cells. Few double-labeled cells are present.
|
Specimen
3J:
embryonic day 7.0; Mesp1tm1.1Sjar/Mesp1 +
(more )
Note: Triple labeled: green - Mesp1 reporter; red - Foxa2 (assay MGI:6713524); blue - Dapi.
Specimen
3J:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * NMRI
|
|
Mutant Allele(s):
|
Mesp1tm1.1Sjar/Mesp1+
|
|
Age:
|
embryonic day 7.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Triple labeled: green - Mesp1 reporter; red - Foxa2 (assay MGI:6713524); blue - Dapi.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS10: embryo mesoderm |
Present |
Regionally restricted |
3J
|
At distal tip of primitive streak, no expressing cells within streak or delaminated cells.
|
| TS10: primitive streak |
Present |
Regionally restricted |
3J
|
At distal tip of primitive streak, no expressing cells within streak or delaminated cells.
|
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Analysis Tools