Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:301247
Probst S, et al., Spatiotemporal sequence of mesoderm and endoderm lineage segregation during mouse gastrulation. Development. 2021 Jan 7;148(1):dev193789
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:6713524
|
|
Gene symbol:
|
Foxa2
|
|
Gene name:
|
forkhead box A2
|
|
|
Results
|
Specimen
3A:
embryonic day 6.5; Mesp1tm1.1Sjar/Mesp1 +
(more  )
Note: Triple labeled: red - Foxa2 immunohistochemistry; green - Mesp1 reporter (assay MGI:6713515); blue - Dapi.
Specimen
3A:
(close  )
|
Genetic Background:
|
involves: 129S/SvEv * NMRI
|
|
Mutant Allele(s):
|
Mesp1tm1.1Sjar/Mesp1+
|
|
Age:
|
embryonic day 6.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Triple labeled: red - Foxa2 immunohistochemistry; green - Mesp1 reporter (assay MGI:6713515); blue - Dapi.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS9: embryo ectoderm |
Present |
Regionally restricted |
3A
|
Expression in definitive endoderm progenitors within posterior epiblast, located more distally in relation to proximally located Mesp1-expressing anterior mesoderm cells.
|
Specimen
3B:
embryonic day 6.75; Mesp1tm1.1Sjar/Mesp1 +
(more )
Note: Triple labeled: red - Foxa2 immunohistochemistry; green - Mesp1 reporter (assay MGI:6713515); blue - Dapi.
Specimen
3B:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * NMRI
|
|
Mutant Allele(s):
|
Mesp1tm1.1Sjar/Mesp1+
|
|
Age:
|
embryonic day 6.75
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Triple labeled: red - Foxa2 immunohistochemistry; green - Mesp1 reporter (assay MGI:6713515); blue - Dapi.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS10: primitive endoderm |
Present |
Widespread |
3B
|
Expression in visceral endoderm covering whole embryo.
|
| TS10: embryo ectoderm |
Present |
Regionally restricted |
3B
|
Expressing cells in posterior distal epiblast are especially dense, probably corresponding to definitive endoderm precursors.
|
Specimen
3C:
embryonic day 6.5; Mesp1tm1.1Sjar/Mesp1 +
(more )
Note: Triple labeled: red - Foxa2 immunohistochemistry; green - Mesp1 reporter (assay MGI:6713515); blue - Dapi.
Specimen
3C:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * NMRI
|
|
Mutant Allele(s):
|
Mesp1tm1.1Sjar/Mesp1+
|
|
Age:
|
embryonic day 6.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Triple labeled: red - Foxa2 immunohistochemistry; green - Mesp1 reporter (assay MGI:6713515); blue - Dapi.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS9: primitive endoderm |
Present |
|
3C
|
Expression in visceral endoderm cells.
|
| TS9: embryo ectoderm |
Present |
|
3C
|
Expression in definitive endoderm progenitors remaining within epithelial epiblast, located more distally in relation to Mesp1-expressing anterior mesoderm cells.
|
Specimen
3D:
embryonic day 6.5; Mesp1tm1.1Sjar/Mesp1 +
(more )
Note: Triple labeled: red - Foxa2 immunohistochemistry; green - Mesp1 reporter (assay MGI:6713515); blue - Dapi.
Specimen
3D:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * NMRI
|
|
Mutant Allele(s):
|
Mesp1tm1.1Sjar/Mesp1+
|
|
Age:
|
embryonic day 6.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Triple labeled: red - Foxa2 immunohistochemistry; green - Mesp1 reporter (assay MGI:6713515); blue - Dapi.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS9: embryo ectoderm |
Present |
Single cells |
3D
|
Single expressing cells in Mesp1-expressing domain of epiblast.
|
Specimen
3E:
embryonic day 6.5; Mesp1tm1.1Sjar/Mesp1 +
(more )
Note: Triple labeled: red - Foxa2 immunohistochemistry; green - Mesp1 reporter (assay MGI:6713515); blue - Dapi.
Specimen
3E:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * NMRI
|
|
Mutant Allele(s):
|
Mesp1tm1.1Sjar/Mesp1+
|
|
Age:
|
embryonic day 6.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Triple labeled: red - Foxa2 immunohistochemistry; green - Mesp1 reporter (assay MGI:6713515); blue - Dapi.
|
Specimen
3F:
embryonic day 6.5; Mesp1tm1.1Sjar/Mesp1 +
(more )
Note: Triple labeled: red - Foxa2 immunohistochemistry; green - Mesp1 reporter (assay MGI:6713515); blue - Dapi.
Specimen
3F:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * NMRI
|
|
Mutant Allele(s):
|
Mesp1tm1.1Sjar/Mesp1+
|
|
Age:
|
embryonic day 6.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Triple labeled: red - Foxa2 immunohistochemistry; green - Mesp1 reporter (assay MGI:6713515); blue - Dapi.
|
Specimen
3G:
embryonic day 7.0; Mesp1tm1.1Sjar/Mesp1 +
(more )
Note: Triple labeled: red - Foxa2 immunohistochemistry; green - Mesp1 reporter (assay MGI:6713515); blue - Dapi.
Specimen
3G:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * NMRI
|
|
Mutant Allele(s):
|
Mesp1tm1.1Sjar/Mesp1+
|
|
Age:
|
embryonic day 7.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Triple labeled: red - Foxa2 immunohistochemistry; green - Mesp1 reporter (assay MGI:6713515); blue - Dapi.
|
Specimen
3H:
embryonic day 7.0; Mesp1tm1.1Sjar/Mesp1 +
(more )
Note: Triple labeled: red - Foxa2 immunohistochemistry; green - Mesp1 reporter (assay MGI:6713515); blue - Dapi.
Specimen
3H:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * NMRI
|
|
Mutant Allele(s):
|
Mesp1tm1.1Sjar/Mesp1+
|
|
Age:
|
embryonic day 7.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Triple labeled: red - Foxa2 immunohistochemistry; green - Mesp1 reporter (assay MGI:6713515); blue - Dapi.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS10: primitive endoderm |
Present |
|
3H
|
Expression in visceral endoderm cells.
|
| TS10: embryo ectoderm |
Present |
Regionally restricted |
3H
|
Expression in definitive endoderm progenitors. Expression in single cells intermixed with single Mesp1-expressing cells.
|
Specimen
3I:
embryonic day 7.0; Mesp1tm1.1Sjar/Mesp1 +
(more )
Note: Triple labeled: red - Foxa2 immunohistochemistry; green - Mesp1 reporter (assay MGI:6713515); blue - Dapi.
Specimen
3I:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * NMRI
|
|
Mutant Allele(s):
|
Mesp1tm1.1Sjar/Mesp1+
|
|
Age:
|
embryonic day 7.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Triple labeled: red - Foxa2 immunohistochemistry; green - Mesp1 reporter (assay MGI:6713515); blue - Dapi.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS10: primitive endoderm |
Present |
|
3I
|
Expression in visceral endoderm cells.
|
| TS10: embryo ectoderm |
Present |
Regionally restricted |
3I
|
Expression in definitive endoderm progenitors. Expression in single cells intermixed with single Mesp1-expressing cells.
|
Specimen
3J:
embryonic day 7.0; Mesp1tm1.1Sjar/Mesp1 +
(more )
Note: Triple labeled: red - Foxa2 immunohistochemistry; green - Mesp1 reporter (assay MGI:6713515); blue - Dapi.
Specimen
3J:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * NMRI
|
|
Mutant Allele(s):
|
Mesp1tm1.1Sjar/Mesp1+
|
|
Age:
|
embryonic day 7.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Triple labeled: red - Foxa2 immunohistochemistry; green - Mesp1 reporter (assay MGI:6713515); blue - Dapi.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS10: primitive streak |
Present |
Regionally restricted |
3J
|
At distal tip of streak, only Foxa2-expressing cells within streak or delaminated cells.
|
|
Analysis Tools