Gene Expression Data
Assay Details
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Assay
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Reference:
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J:297547
Leca I, et al., A proteomic survey of microtubule-associated proteins in a R402H TUBA1A mutant mouse. PLoS Genet. 2020 Nov;16(11):e1009104
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6695146
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Gene symbol:
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Pou3f2
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Gene name:
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POU domain, class 3, transcription factor 2
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Antibody:
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anti-Brn2 (sc-393324)
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Assay notes:
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Specimens were subjected to heat-mediated antigen retrieval.
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Results
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Specimen
4P:
embryonic day 18.5; Emx1tm1(cre)Krj/Emx1 +
(more  )
Note: coronal section
Specimen
4P:
(close  )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6J
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Mutant Allele(s):
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Emx1tm1(cre)Krj/Emx1+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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coronal section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cerebral cortex |
Present |
Regionally restricted |
4P
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Expression is seen in upper layer neurons.
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Specimen
4Q:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6J
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Mutant Allele(s):
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Tuba1atm1.1Dak/Tuba1a+
Emx1tm1(cre)Krj/Emx1+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Conditional mutant. coronal section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cerebral cortex |
Present |
Regionally restricted |
4Q
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Expression is seen in upper layer neurons. The number of expressing cells in the heterozygote is comparable to that seen in controls.
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Specimen
4R:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6J
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Mutant Allele(s):
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Tuba1atm1.1Dak/Tuba1atm1.1Dak
Emx1tm1(cre)Krj/Emx1+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Conditional mutant. coronal section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: cerebral cortex intermediate zone |
Present |
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4R
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Unlike in the controls, where staining is seen in upper layer neurons, in the homozygote neurons accumulate in the intermediate zone. In addition there is a significant reduction in the number of staining cells.
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Analysis Tools