Probe:	Insm1 probe1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	1A	Expressed around the ventricle. Expressed in a band of cells a short distance away from the apical surface of the ventricles. In addition, in cortex there was a second band of cells closer to the ventricle.
TS20: hindbrain	Present	Regionally restricted	1A	Expressed around the ventricle. Expressed in a band of cells a short distance away from the apical surface of the ventricles. In addition, in cortex there was a second band of cells closer to the ventricle.
TS20: telencephalon	Present	Regionally restricted	1A	Expressed around the ventricle. Expressed in a band of cells a short distance away from the apical surface of the ventricles. In addition, in cortex there was a second band of cells closer to the ventricle.
TS20: spinal cord	Present	Regionally restricted	1A	Expressed in the ependymal layer.
TS20: lateral ganglionic eminence	Present		1A
TS20: medial ganglionic eminence	Present		1A

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	1B	Expressed around the ventricle. Expressed in a band of cells a short distance away from the apical surface of the ventricles. In addition, in cortex there was a second band of cells closer to the ventricle.
TS20: telencephalon	Present	Regionally restricted	1B	Expressed around the ventricle. Expressed in a band of cells a short distance away from the apical surface of the ventricles. In addition, in cortex there was a second band of cells closer to the ventricle.
TS20: spinal cord	Present	Regionally restricted	1B	Expressed in the ependymal layer.
TS20: lateral ganglionic eminence	Present		1B
TS20: medial ganglionic eminence	Present		1B
TS20: spinal ganglion	Present		1B
TS20: trigeminal ganglion	Present		1B

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted	4C , 4A	Not expressed in apical dividing cells and from most basal postmitotic cells, but expressed in a band of cells that included some of the most basal dividing precursors and some of the adjacent postmitotic cells.

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Present		4E	About half of the dividing cells expressed Insml and about half did not.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	5A , 5C	Absent from the more apical S-phase cells and from the most basal postmitotic cells, but was present in a band of cells that included some of the most basal dividing progenitors and some of the adjacent, least basal BrdU-negative cells.

Structure	Level	Pattern	Image	Note
TS22: ganglionic eminence	Present	Regionally restricted	6C , 6A	Expression was absent from the apical cells, and thus from the surface mitoses of the ventricular zone, but was present in a band of cells that includes the basal, nonsurface mitotic precursors of the subventricular zone.

Structure	Level	Pattern	Image	Note
TS21: lateral ganglionic eminence	Present	Regionally restricted	6E	Expression was absent from the apical cells, and thus from the surface mitoses of the ventricular zone, but was present in a band of cells that includes the basal, nonsurface mitotic precursors of the subventricular zone.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	7A	Expressed by the more basal progenitors (BrdU-positive) and by some adjacent BrdU-negntive cells, which could be nonsurface progenitors in G1 or nascent neurons.

Structure	Level	Pattern	Image	Note
TS20: neocortex	Present	Regionally restricted	7C	Expression overlaped with the population of progenitors in S-phase that were more basally located and thus probably were the secondary proliferative population.
TS20: cortical plate	Absent		7C

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex ventricular layer	Present	Regionally restricted	7E	Expressed in more cells close to the ventricles, but absent from the apical cells, including those undergoing surface mitoses.
TS21: cerebral cortex subventricular zone	Present	Regionally restricted	7E	Expressed in a band of cells that includes the basal, nonsurface mitotic precursors.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Present		8A , 8D	Expressed in both dividing progenitors (Mki67-positive) and nondividing cells (Mki67-negative), which were abundant basally.
TS22: lateral ganglionic eminence	Present		8A

Structure	Level	Pattern	Image	Note
TS21: cortical plate	Present	Regionally restricted	8B	There were a few cells co-expressed with Dcx in the basal cortical plate. Mostly not co-expressed with Dcx.

Structure	Level	Pattern	Image	Note
TS21: cortical plate	Present		8G , 8I	Many cells co-expressed with Eomes, while others were Eomes-negative.

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present	Regionally restricted	9A	Expressed in basal mitotic cells (neurogenic progenitors), but not in apical mitotic sustentacular cells.

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present	Regionally restricted	9C	Expressed in basal but not apical progenitors. Also expressed in postmitotic olfactory receptor neurons in the middle of the epithelium.

Structure	Level	Pattern	Image	Note
TS22: vomeronasal organ	Present	Scattered	9E	Expressed in cells scattered throughout the vomeronasal organ, many of them in the basal part of the sensory half. Some were in mitosis.

Structure	Level	Pattern	Image	Note
TS22: neural retina	Present	Regionally restricted	10A	Expressed by about half of all dividing cells of the outer neuroblastic layer and by some of the adjacent postmitotic cells of the ganglion cell layer but not in the most basal cells.

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Present		11C	Expressed in proliferating cells of the outer external granule cell layer and in postmitotic precursors of the inner external granule cell layer.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex subventricular zone	Present		12A
TS28: rostral migratory stream	Present		12A

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex subventricular zone	Present		12B

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex subventricular zone	Present		12D

Structure	Level	Pattern	Image	Note
TS28: rostral migratory stream	Present		12F	Expressed in dividing cells (Mki67+) and non-dividing cells.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Present		13A
TS28: rostral migratory stream	Present		13A
TS28: main olfactory bulb granule cell layer	Weak	Scattered	13A
TS28: main olfactory bulb external plexiform layer	Absent		13A
TS28: main olfactory bulb mitral cell layer	Absent		13A

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Present		13E

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus subgranular zone	Present		14A	Expressed in dividing cells.