Gene Expression Data
Assay Details
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Assay
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Reference:
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J:301719
Su J, et al., ZC3H4-a novel Cys-Cys-Cys-His-type zinc finger protein-is essential for early embryogenesis in mice. Biol Reprod. 2021 Feb 11;104(2):325-335
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6515650
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Gene symbol:
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Pou5f1
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Gene name:
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POU domain, class 5, transcription factor 1
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Results
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Specimen
S3 littermate 1:
embryonic day 5.5; Zc3h4em1(IMPC)Mbp/Zc3h4 +
(more  )
Note: This embryo could be a homozygous wild-type or a heterozygote. Triple labeled: green - Pou5f1; red - Cdx2 (assay MGI:6515655); white - laminin.
Specimen
S3 littermate 1:
(close  )
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Genetic Background:
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C57BL/6NCrl-Zc3h4em1(IMPC)Mbp/Mmucd
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Mutant Allele(s):
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Zc3h4em1(IMPC)Mbp/Zc3h4+
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Age:
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embryonic day 5.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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This embryo could be a homozygous wild-type or a heterozygote. Triple labeled: green - Pou5f1; red - Cdx2 (assay MGI:6515655); white - laminin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS8: embryo |
Present |
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S3_littermate 1 Merged
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S3_littermate 1 OCT4
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This is a normal embryo with appropriate expression.
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Specimen
S3 littermate 1, mother:
postnatal adult; Zc3h4em1(IMPC)Mbp/Zc3h4 +
(more )
Note: Triple labeled: green - Pou5f1; red - Cdx2 (assay MGI:6515655); white - laminin.
Specimen
S3 littermate 1, mother:
(close )
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Genetic Background:
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C57BL/6NCrl-Zc3h4em1(IMPC)Mbp/Mmucd
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Mutant Allele(s):
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Zc3h4em1(IMPC)Mbp/Zc3h4+
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: green - Pou5f1; red - Cdx2 (assay MGI:6515655); white - laminin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: decidua |
Present |
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S3_littermate 1 Merged
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S3_littermate 1 OCT4
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Specimen
S3 littermate 2:
embryonic day 5.5; Zc3h4em1(IMPC)Mbp/Zc3h4 +
(more )
Note: This embryo could be a homozygous wild-type or a heterozygote. Triple labeled: green - Pou5f1; red - Cdx2 (assay MGI:6515655); white - laminin.
Specimen
S3 littermate 2:
(close )
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Genetic Background:
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C57BL/6NCrl-Zc3h4em1(IMPC)Mbp/Mmucd
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Mutant Allele(s):
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Zc3h4em1(IMPC)Mbp/Zc3h4+
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Age:
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embryonic day 5.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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This embryo could be a homozygous wild-type or a heterozygote. Triple labeled: green - Pou5f1; red - Cdx2 (assay MGI:6515655); white - laminin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS8: embryo |
Present |
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S3_littermate 2 Merged
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S3_littermate 2 OCT4
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This is a normal embryo with appropriate expression.
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Specimen
S3 littermate 2, mother:
postnatal adult; Zc3h4em1(IMPC)Mbp/Zc3h4 +
(more )
Note: Triple labeled: green - Pou5f1; red - Cdx2 (assay MGI:6515655); white - laminin.
Specimen
S3 littermate 2, mother:
(close )
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Genetic Background:
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C57BL/6NCrl-Zc3h4em1(IMPC)Mbp/Mmucd
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Mutant Allele(s):
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Zc3h4em1(IMPC)Mbp/Zc3h4+
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: green - Pou5f1; red - Cdx2 (assay MGI:6515655); white - laminin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: decidua |
Present |
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S3_littermate 2 OCT4
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S3_littermate 2 Merged
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Specimen
S3 presumed mutant 1:
postnatal adult; Zc3h4em1(IMPC)Mbp/Zc3h4 +
(more )
Note: Triple labeled: green - Pou5f1; red - Cdx2 (assay MGI:6515655); white - laminin.
Specimen
S3 presumed mutant 1:
(close )
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Genetic Background:
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C57BL/6NCrl-Zc3h4em1(IMPC)Mbp/Mmucd
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Mutant Allele(s):
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Zc3h4em1(IMPC)Mbp/Zc3h4+
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: green - Pou5f1; red - Cdx2 (assay MGI:6515655); white - laminin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: decidua |
Present |
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S3_presumed mutant 1 Merged
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S3_presumed mutant 1 OCT4
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This decidua had tiny round structures in the midst of normal sized maternal decidual cells which were absent in expression. The authors presumed that these were remnants of the Zc3h4 mutant embryos and did not detail expression in the decidua.
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Specimen
S3 presumed mutant 2:
postnatal adult; Zc3h4em1(IMPC)Mbp/Zc3h4 +
(more )
Note: Triple labeled: green - Pou5f1; red - Cdx2 (assay MGI:6515655); white - laminin.
Specimen
S3 presumed mutant 2:
(close )
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Genetic Background:
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C57BL/6NCrl-Zc3h4em1(IMPC)Mbp/Mmucd
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Mutant Allele(s):
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Zc3h4em1(IMPC)Mbp/Zc3h4+
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: green - Pou5f1; red - Cdx2 (assay MGI:6515655); white - laminin.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: decidua |
Present |
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S3_presumed mutant 2 OCT4
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S3_presumed mutant 2 Merged
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This decidua had tiny round structures in the midst of normal sized maternal decidual cells which were absent in expression. The authors presumed that these were remnants of the Zc3h4 mutant embryos and did not detail expression in the decidua.
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Analysis Tools