Reporter:	lacZ
	Direct Detection

Structure	Level	Pattern	Image	Note
TS14: otic pit epithelium	Present			Expression detectable in otic epithelium at E9.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present	Regionally restricted	1A	Expression in ventral-lateral wall of posterior otocyst maintained and extended at E9.5.

Structure	Level	Pattern	Image	Note
TS15: otocyst epithelium	Absent		1A'	Otocyst forms but is smaller than in wildtype. No expression in otic epithelium

Structure	Level	Pattern	Image	Note
TS17: otocyst	Present	Regionally restricted	1B	Expression throughout lateral otocyst wall.
TS17: otocyst epithelium	Present	Widespread	1B	Widely expressed in otic epithelium, but excluded from most dorsal and medial regions.
TS17: endolymphatic appendage	Absent		1B	No expression in endolymphatic duct.

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Absent		1B'	Otocyst forms but is smaller than in wildtype. No expression in otic epithelium.

Structure	Level	Pattern	Image	Note
TS17: 2nd branchial arch mesenchyme	Present		1C	Expression in periotic mesenchyme where it is continuous with expression in mesenchyme of second pharyngeal arch.
TS17: otocyst mesenchyme	Present		1C	Expression in periotic mesenchyme where it is continuous with expression in mesenchyme of second pharyngeal arch.

Structure	Level	Pattern	Image	Note
TS20: semicircular canal	Present	Regionally restricted	1D	Expression delineates developing semicircular canals.

Structure	Level	Pattern	Image	Note
TS20: inner ear epithelium	Absent		1D'	No expression in otic epithelium.

Structure	Level	Pattern	Image	Note
TS21: cochlea	Present	Regionally restricted	1E	Expression delineates developing cochlea.

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Present	Regionally restricted	2G	Widely expressed in otic epithelium, but excluded from most dorsal and medial regions, including endolymphatic duct.

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Present	Regionally restricted	2H	Widely expressed in otic epithelium, but excluded from most dorsal and medial regions, including endolymphatic duct.

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Present	Regionally restricted	2I	Widely expressed in otic epithelium, but excluded from most dorsal and medial regions, including endolymphatic duct.

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Present	Regionally restricted	2J	Widely expressed in otic epithelium, but excluded from most dorsal and medial regions, including endolymphatic duct.

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Present	Regionally restricted	2K	Widely expressed in otic epithelium, but excluded from most dorsal and medial regions, including endolymphatic duct.

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Present	Regionally restricted	2L	Widely expressed in otic epithelium, but excluded from most dorsal and medial regions, including endolymphatic duct.

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Present	Regionally restricted	2M	Widely expressed in otic epithelium, but excluded from most dorsal and medial regions, including endolymphatic duct.

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Present	Regionally restricted	2N	Widely expressed in otic epithelium, but excluded from most dorsal and medial regions, including endolymphatic duct.

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Present	Regionally restricted	2O	Widely expressed in otic epithelium, but excluded from most dorsal and medial regions, including endolymphatic duct.

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Present	Regionally restricted	2P	Widely expressed in otic epithelium, but excluded from most dorsal and medial regions, including endolymphatic duct.

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Present	Regionally restricted	2Q	Widely expressed in otic epithelium, but excluded from most dorsal and medial regions, including endolymphatic duct.

Structure	Level	Pattern	Image	Note
TS17: otocyst epithelium	Present	Regionally restricted	2R	Widely expressed in otic epithelium, but excluded from most dorsal and medial regions, including endolymphatic duct.

Structure	Level	Pattern	Image	Note
TS14: otic pit epithelium	Present		4A	Expression in otocyst epithelial cells.

Structure	Level	Pattern	Image	Note
TS14: otic pit epithelium	Present	Regionally restricted	4A'	Expression in a small population of epithelial cells in posterior otocyst.

Structure	Level	Pattern	Image	Note
TS14: otic pit epithelium	Present	Regionally restricted	4B	Expression localized to lateral wall of posterior otocyst.

Structure	Level	Pattern	Image	Note
TS14: otic pit epithelium	Present	Regionally restricted	4B'	Expression in a small population of epithelial cells localized to lateral wall of posterior otocyst.