Six2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:280280 Rutledge EA, et al., Morphogenesis of the kidney and lung requires branch-tip directed activity of the Adamts18 metalloprotease. Dev Biol. 2019 Oct 15;454(2):156-169
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6512059
Gene symbol:	Six2
Gene name:	sine oculis-related homeobox 2

Antibody:	anti-Six2 (11562-1-AP)
Detection system:	Secondary antibody

Results

8B ©

Specimen 8B: embryonic day 12.5 (more

)
Note: 50-52 somite-stage embryos. Double labeled: red - Six2; white - pan-cytokeratin immunostaining.

Structure	Level	Pattern	Image	Note
TS20: cap mesenchyme	Present	Regionally restricted	8B	Expression is seen in nephron progenitors; double ureters are observed in 2% of individuals.

8D ©

Specimen 8D: embryonic day 12.5; Adamts18^{tm1(KOMP)Vlcg}/Adamts18⁺ (more

)
Note: 50-52 somite-stage embryos. Double labeled: red - Six2; white - pan-cytokeratin immunostaining.

Structure	Level	Pattern	Image	Note
TS20: cap mesenchyme	Present	Regionally restricted	8D	Expression is seen in nephron progenitors; double ureters are observed in 3% of individuals.

8F ©

Specimen 8F: embryonic day 12.5; Adamts18^{tm1(KOMP)Vlcg}/Adamts18^{tm1(KOMP)Vlcg} (more

)
Note: 50-52 somite-stage embryos. Double labeled: red - Six2; white - pan-cytokeratin immunostaining.

Structure	Level	Pattern	Image	Note
TS20: cap mesenchyme	Present	Regionally restricted	8F	Expression is seen in nephron progenitors; double ureters are observed in 38% of individuals.

8H ©

Specimen 8H: embryonic day 12.5; Adamts16^em1Amc/Adamts16^em1Amc, Adamts18^{tm1(KOMP)Vlcg}/Adamts18^{tm1(KOMP)Vlcg} (more

)
Note: 50-52 somite-stage embryos. Double labeled: red - Six2; white - pan-cytokeratin immunostaining.

Structure	Level	Pattern	Image	Note
TS20: cap mesenchyme	Present	Regionally restricted	8H	Expression is seen in nephron progenitors; double ureters are observed in 35% of individuals.

S3A ©

Specimen S3A: embryonic day 12.5 (more

)
Note: Double labeled: red - Six2; white - pan-cytokeratin immunostaining.

Structure	Level	Pattern	Image	Note
TS20: lung	Present		S3A

S3B ©

Specimen S3B: embryonic day 12.5; Adamts16^em1Amc/Adamts16^em1Amc (more

)
Note: Double labeled: red - Six2; white - pan-cytokeratin immunostaining.

Structure	Level	Pattern	Image	Note
TS20: lung	Present		S3B	Expression in the mutant is the same as in wild type.

S3C ©

Specimen S3C: embryonic day 12.5; Adamts18^{tm1(KOMP)Vlcg}/Adamts18^{tm1(KOMP)Vlcg} (more

)
Note: Double labeled: red - Six2; white - pan-cytokeratin immunostaining.

Structure	Level	Pattern	Image	Note
TS20: lung	Present		S3C	Abnormalities are noted in the mutant lung.

S3D ©

Specimen S3D: embryonic day 12.5; Adamts16^em1Amc/Adamts16^em1Amc, Adamts18^{tm1(KOMP)Vlcg}/Adamts18^{tm1(KOMP)Vlcg} (more

)
Note: Double labeled: red - Six2; white - pan-cytokeratin immunostaining.

Structure	Level	Pattern	Image	Note
TS20: lung	Present		S3D	Abnormalities are noted in the mutant lung.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24