Antibody:

Anti-Gata3 (H-48; sc-9009)

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage embryo	Absent		4A Gata3/Nuclei , 4A Gata3

Structure	Level	Pattern	Image	Note
TS3: compacted morula	Present		4B Gata3/Nuclei , 4B Gata3	Expression correlated closely, but not completely with Cdx2.

Structure	Level	Pattern	Image	Note
TS4: compacted morula	Present		4C Gata3/Nuclei , 4C Gata3	Expression correlated closely, but not completely with Cdx2.

Structure	Level	Pattern	Image	Note
TS4: trophectoderm	Present	Regionally restricted	4D Gata3/Nuclei , 4D Gata3	Expression was detectable within the nucleiof the trophectoderm, where it colocalized with Cdx2.

Structure	Level	Pattern	Image	Note
TS4: trophectoderm	Present		6A Gata3 , 6A Gata3/Nuclei

Structure	Level	Pattern	Image	Note
TS4: trophectoderm	Trace		6B Gata3 , 6B Gata3/Nuclei	Nuclear levels of Gata3 were greatly reduced in Tead4 mutants. Low levels of Gata3 could be detected in the nuclei of some cells.

Structure	Level	Pattern	Image	Note
TS4: trophectoderm	Present		6C Gata3/Nuclei , 6C Gata3

Structure	Level	Pattern	Image	Note
TS4: trophectoderm	Present		6D Gata3 , 6D Gata3/Nuclei	Gata3 expression was unaffected by the loss of Cdx2.

Structure	Level	Pattern	Image	Note
TS4: trophectoderm	Present		7C Gata3	Gata3 expression pattern was largely unaffected in Pou5f1 mutants.
TS4: inner cell mass	Absent		7C Gata3

Structure	Level	Pattern	Image	Note
TS6: epiblast	Absent		7D Gata3	Expression was excluded from nuclei.
TS6: primitive endoderm	Absent		7D Gata3	Expression was excluded from nuclei.

Structure	Level	Pattern	Image	Note
TS6: epiblast	Present		7E Gata3	Nuclear expression of Gata3 was upregulated in the mutant epiblast and primitive endoderm.
TS6: primitive endoderm	Present		7E Gata3	Nuclear expression of Gata3 was upregulated in the mutant epiblast and primitive endoderm.