Gene Expression Data
Assay Details
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Assay
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Reference:
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J:54582
Iacopetti P, et al., Expression of the antiproliferative gene TIS21 at the onset of neurogenesis identifies single neuroepithelial cells that switch from proliferative to neuron-generating division. Proc Natl Acad Sci U S A. 1999 Apr 13;96(8):4639-44
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6508686
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Gene symbol:
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Btg2
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Gene name:
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BTG anti-proliferation factor 2
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Probe:
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TIS21 cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
2A/C:
embryonic day 10.0
(more  )
Note: Double labeled: purple - Btg2; brown - Tubb3 (assay MGI:6508687). Data was quantified in Fig 3.
Specimen
2A/C:
(close  )
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Genetic Background:
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NMRI
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: purple - Btg2; brown - Tubb3 (assay MGI:6508687). Data was quantified in Fig 3.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: diencephalon |
Present |
Single cells |
2C
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2A
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Expressed in only a few cells in the neuroepithelium in regions where neurogenesis was just starting (a few Tubb3+ cells).
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Specimen
2B/D:
embryonic day 10.0
(more )
Note: Double labeled: purple - Btg2; brown - Tubb3 (assay MGI:6508687). Data was quantified in Fig 3.
Specimen
2B/D:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: purple - Btg2; brown - Tubb3 (assay MGI:6508687). Data was quantified in Fig 3.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: future hindbrain |
Present |
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2D
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2B
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Expressed in many more cells in the neuroepithelium (Tubb3+).
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Specimen
not shown:
embryonic day 10.0
(more )
Note: Also stained for Tubb3 (assay MGI:6508687). Data was quantified in Fig 3.
Specimen
not shown:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Also stained for Tubb3 (assay MGI:6508687). Data was quantified in Fig 3.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: future midbrain |
Present |
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Increased expression compared with the diencephalon.
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Specimen
5A/C:
embryonic day 10.0
(more )
Note: Double labeled: purple - Btg2; brown - Btg2 (assay MGI:6509041).
Specimen
5A/C:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: purple - Btg2; brown - Btg2 (assay MGI:6509041).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: diencephalon lateral wall ventricular layer |
Present |
Regionally restricted |
5C
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5A
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Expressed in the perikaryon of cells in the neuroepithelium, which also contained protein in the perikaryon and, more prominently, in cell processes.
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Specimen
5B/D:
embryonic day 10.0
(more )
Note: Double labeled: purple - Btg2; brown - Btg2 (assay MGI:6509041).
Specimen
5B/D:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: purple - Btg2; brown - Btg2 (assay MGI:6509041).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: future hindbrain ventricular layer |
Present |
Regionally restricted |
5D
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5B
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Expressed in the perikaryon of cells in the neuroepithelium, which also contained protein in the perikaryon and, more prominently, in cell processes.
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Specimen
6C:
embryonic day 10.0
(more )
Note: Double labeled: purple - Btg2; brown - Tubb3 (assay MGI:6508687).
Specimen
6C:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
|
Note:
|
Double labeled: purple - Btg2; brown - Tubb3 (assay MGI:6508687).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: future hindbrain ventricular layer |
Present |
Regionally restricted |
6C
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Expressed in the neuroepithelial cells. The newborn neurons (Tubb3+) did not contain detectable levels of expression.
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Specimen
6D:
embryonic day 10.0
(more )
Note: Double labeled: purple - Btg2; brown - Tubb3 (assay MGI:6508687).
Specimen
6D:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
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Note:
|
Double labeled: purple - Btg2; brown - Tubb3 (assay MGI:6508687).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: future hindbrain ventricular layer |
Present |
Regionally restricted |
6D
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Expressed in the neuroepithelial cells. The newborn neurons (Tubb3+) did not contain detectable levels of expression, including those migrating through the ventricular zone and in close proximity to the apical surface.
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Specimen
7H1:
embryonic day 10.0
(more )
Note: Double labeled: purple - Btg2; brown - anti-mitotic proteins antibody.
Specimen
7H1:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: purple - Btg2; brown - anti-mitotic proteins antibody.
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Specimen
7H2:
embryonic day 10.0
(more )
Note: Double labeled: purple - Btg2; brown - anti-mitotic proteins antibody.
Specimen
7H2:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: purple - Btg2; brown - anti-mitotic proteins antibody.
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Specimen
7I:
embryonic day 10.0
(more )
Note: Double labeled: purple - Btg2; brown - anti-BrdU antibody.
Specimen
7I:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: purple - Btg2; brown - anti-BrdU antibody.
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Specimen
7I inset:
embryonic day 10.0
(more )
Note: Double labeled: purple - Btg2; brown - anti-BrdU antibody.
Specimen
7I inset:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: purple - Btg2; brown - anti-BrdU antibody.
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Specimen
7J:
embryonic day 10.0
(more )
Note: Double labeled: purple - Btg2; brown - anti-BrdU antibody.
Specimen
7J:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: purple - Btg2; brown - anti-BrdU antibody.
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Specimen
7J inset:
embryonic day 10.0
(more )
Note: Double labeled: purple - Btg2; brown - anti-BrdU antibody.
Specimen
7J inset:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: purple - Btg2; brown - anti-BrdU antibody.
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Analysis Tools