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Gene Expression Data
Assay Details
Assay
Reference: J:54582 Iacopetti P, et al., Expression of the antiproliferative gene TIS21 at the onset of neurogenesis identifies single neuroepithelial cells that switch from proliferative to neuron-generating division. Proc Natl Acad Sci U S A. 1999 Apr 13;96(8):4639-44
Assay type: RNA in situ
MGI Accession ID: MGI:6508686
Gene symbol: Btg2
Gene name: BTG anti-proliferation factor 2
Probe: TIS21 cDNA2
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 2A/C: embryonic day 10.0 (more )
Note: Double labeled: purple - Btg2; brown - Tubb3 (assay MGI:6508687). Data was quantified in Fig 3.
Structure Level Pattern Image Note
TS16: diencephalon Present Single cells 2C , 2A Expressed in only a few cells in the neuroepithelium in regions where neurogenesis was just starting (a few Tubb3+ cells).

Specimen 2B/D: embryonic day 10.0 (more )
Note: Double labeled: purple - Btg2; brown - Tubb3 (assay MGI:6508687). Data was quantified in Fig 3.
Structure Level Pattern Image Note
TS16: future hindbrain Present 2D , 2B Expressed in many more cells in the neuroepithelium (Tubb3+).

Specimen not shown: embryonic day 10.0 (more )
Note: Also stained for Tubb3 (assay MGI:6508687). Data was quantified in Fig 3.
Structure Level Pattern Image Note
TS16: future midbrain Present Increased expression compared with the diencephalon.

Specimen 5A/C: embryonic day 10.0 (more )
Note: Double labeled: purple - Btg2; brown - Btg2 (assay MGI:6509041).
Structure Level Pattern Image Note
TS16: diencephalon lateral wall ventricular layer Present Regionally restricted 5C , 5A Expressed in the perikaryon of cells in the neuroepithelium, which also contained protein in the perikaryon and, more prominently, in cell processes.

Specimen 5B/D: embryonic day 10.0 (more )
Note: Double labeled: purple - Btg2; brown - Btg2 (assay MGI:6509041).
Structure Level Pattern Image Note
TS16: future hindbrain ventricular layer Present Regionally restricted 5D , 5B Expressed in the perikaryon of cells in the neuroepithelium, which also contained protein in the perikaryon and, more prominently, in cell processes.

Specimen 6C: embryonic day 10.0 (more )
Note: Double labeled: purple - Btg2; brown - Tubb3 (assay MGI:6508687).
Structure Level Pattern Image Note
TS16: future hindbrain ventricular layer Present Regionally restricted 6C Expressed in the neuroepithelial cells. The newborn neurons (Tubb3+) did not contain detectable levels of expression.

Specimen 6D: embryonic day 10.0 (more )
Note: Double labeled: purple - Btg2; brown - Tubb3 (assay MGI:6508687).
Structure Level Pattern Image Note
TS16: future hindbrain ventricular layer Present Regionally restricted 6D Expressed in the neuroepithelial cells. The newborn neurons (Tubb3+) did not contain detectable levels of expression, including those migrating through the ventricular zone and in close proximity to the apical surface.

Specimen 7H1: embryonic day 10.0 (more )
Note: Double labeled: purple - Btg2; brown - anti-mitotic proteins antibody.
Structure Level Pattern Image Note
TS16: future hindbrain ventricular layer Present 7H1 Not coexpressed with the mitotic markers.

Specimen 7H2: embryonic day 10.0 (more )
Note: Double labeled: purple - Btg2; brown - anti-mitotic proteins antibody.
Structure Level Pattern Image Note
TS16: future hindbrain ventricular layer Present 7H2 Not coexpressed with the mitotic markers.

Specimen 7I: embryonic day 10.0 (more )
Note: Double labeled: purple - Btg2; brown - anti-BrdU antibody.
Structure Level Pattern Image Note
TS16: future hindbrain ventricular layer Present 7I Majority of positive cells were not in S phase.

Specimen 7I inset: embryonic day 10.0 (more )
Note: Double labeled: purple - Btg2; brown - anti-BrdU antibody.
Structure Level Pattern Image Note
TS16: future hindbrain ventricular layer Present 7I inset Occasionally positive cell was in S phase.

Specimen 7J: embryonic day 10.0 (more )
Note: Double labeled: purple - Btg2; brown - anti-BrdU antibody.
Structure Level Pattern Image Note
TS16: future hindbrain ventricular layer Present 7J Majority of positive cells were not in G2 phase.

Specimen 7J inset: embryonic day 10.0 (more )
Note: Double labeled: purple - Btg2; brown - anti-BrdU antibody.
Structure Level Pattern Image Note
TS16: future hindbrain ventricular layer Present 7J inset Occasionally positive cell was in G2 phase.


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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory