Gene Expression Data
Assay Details
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Assay
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Reference:
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J:54582
Iacopetti P, et al., Expression of the antiproliferative gene TIS21 at the onset of neurogenesis identifies single neuroepithelial cells that switch from proliferative to neuron-generating division. Proc Natl Acad Sci U S A. 1999 Apr 13;96(8):4639-44
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6509041
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Gene symbol:
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Btg2
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Gene name:
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BTG anti-proliferation factor 2
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Antibody:
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K4
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Results
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Specimen
5A/C:
embryonic day 10.0
(more  )
Note: Double labeled: brown - Btg2; purple - Btg2 (assay MGI:6508686).
Specimen
5A/C:
(close  )
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Genetic Background:
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NMRI
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Btg2; purple - Btg2 (assay MGI:6508686).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: diencephalon |
Present |
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5C
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5A
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Expressed in the perikaryon and cell processes of the neuroepithelium. Cells containing protein but not mRNA were also observed in the neuroepithelium. Protein but not mRNA was detected in neurons in the mantle zone.
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| TS16: diencephalon lateral wall ventricular layer |
Present |
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5A
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5C
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Expressed in the perikaryon and cell processes of the neuroepithelium. Cells containing protein but not mRNA were also observed in the neuroepithelium.
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Specimen
5B/D:
embryonic day 10.0
(more )
Note: Double labeled: brown - Btg2; purple - Btg2 (assay MGI:6508686).
Specimen
5B/D:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Btg2; purple - Btg2 (assay MGI:6508686).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: future hindbrain |
Present |
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5D
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5B
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Expressed in the perikaryon and cell processes of the neuroepithelium. Cells containing protein but not mRNA were also observed in the neuroepithelium. Protein but not mRNA was detected in neurons in the mantle zone.
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| TS16: future hindbrain ventricular layer |
Present |
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5B
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5D
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Expressed in the perikaryon and cell processes of the neuroepithelium. Cells containing protein but not mRNA were also observed in the neuroepithelium.
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