H2ax Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:297493 Matos-Rodrigues GE, et al., Progenitor death drives retinal dysplasia and neuronal degeneration in a mouse model of ATRIP-Seckel syndrome. Dis Model Mech. 2020 Oct 30;13(10):dmm045807
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6506440
Gene symbol:	H2ax
Gene name:	H2A.X variant histone

Antibody:	anti-gamma H2A.X (phospho S139) (abcam; ab11174)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to Cy3
Assay notes:	Two antibodies were described, but the one used in this assay was not detailed. Either MGI:6430453 or MGI:5305847 was used.

Results

4E Atrip^Ctrl ©

Specimen 4E Atrip^Ctrl: embryonic day 17.5 (more

)

Structure	Level	Pattern	Image	Note
TS25: retina	Ambiguous		4E Atrip^Ctrl

Specimen 4E Atrip^alpha-Cre: embryonic day 17.5; Atrip^tm1.1Pof/Atrip^tm1.1Pof, Tg(Pax6-cre,GFP)2Pgr/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS25: retina	Present		4E Atrip^alpha-Cre	A substantial increase in DNA damage was detected in the mutant retina.

Specimen 4F E12.5 Atrip^Ctrl: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: retina	Ambiguous

Specimen 4F E12.5 Atrip^alpha-Cre: embryonic day 12.5; Atrip^tm1.1Pof/Atrip^tm1.1Pof, Tg(Pax6-cre,GFP)2Pgr/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS20: retina	Ambiguous			No change in DNA damage was detected in the mutant retina.

Specimen 4F P2 Atrip^Ctrl: postnatal day 2 (more

)

Structure	Level	Pattern	Image	Note
TS27: retina	Present

Specimen 4F P2 Atrip^alpha-Cre: postnatal day 2; Atrip^tm1.1Pof/Atrip^tm1.1Pof, Tg(Pax6-cre,GFP)2Pgr/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS27: retina	Present			A modest increase in DNA damage was detected in the mutant retina.

Specimen 4F P4 Atrip^Ctrl: postnatal day 4 (more

)

Structure	Level	Pattern	Image	Note
TS28: retina	Present

Specimen 4F P4 Atrip^alpha-Cre: postnatal day 4; Atrip^tm1.1Pof/Atrip^tm1.1Pof, Tg(Pax6-cre,GFP)2Pgr/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS28: retina	Present

Specimen S3C Atrip^Ctrl: embryonic day 17.5 (more

)

Structure	Level	Pattern	Image	Note
TS25: retina	Ambiguous		S3C Atrip^Ctrl

Specimen S3C Atrip^alpha-Cre: embryonic day 17.5; Atrip^tm1.1Pof/Atrip^tm1.1Pof, Tg(Pax6-cre,GFP)2Pgr/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS25: retina	Present	Regionally restricted	S3C Atrip^alpha-Cre	DNA damage was in the retinal periphery.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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