Pax3 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:122815 Giordani J, et al., Six proteins regulate the activation of Myf5 expression in embryonic mouse limbs. Proc Natl Acad Sci U S A. 2007 Jul 3;104(27):11310-11315
Assay type:	RNA in situ
MGI Accession ID:	MGI:6504945
Gene symbol:	Pax3
Gene name:	paired box 3

Probe:	Pax3 probe54
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

1Aa ©

Specimen 1Aa: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted	1Aa	Expression in myogenic progenitor cells.

1Ab ©

Specimen 1Ab: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud	Present	Regionally restricted	1Ab	Expression in myogenic progenitor cells.
TS17: dermomyotome	Present		1Ab

1Ac ©

Specimen 1Ac: embryonic day 10.5; Six1^tm1Mair/Six1^tm1Mair (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Weak	Regionally restricted	1Ac	Authors report reduced number of myogenic progenitor cells.

1Ad ©

Specimen 1Ad: embryonic day 10.5; Six1^tm1Mair/Six1^tm1Mair (more

)

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud	Weak	Regionally restricted	1Ad	Reduced number of myogenic progenitor cells entering from hypaxial dermomyotome of adjacent somite
TS17: dermomyotome	Present		1Ad

1Ae ©

Specimen 1Ae: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: embryo	Present	Regionally restricted	1Ae

1Af ©

Specimen 1Af: embryonic day 10.5; Six1^tm1Mair/Six1^tm1Mair (more

)

Structure	Level	Pattern	Image	Note
TS17: embryo	Present	Regionally restricted	1Af

1Ah ©

Specimen 1Ah: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	1Ah	Myogenic progenitor cells persent in forelimb buds.

1Aj ©

Specimen 1Aj: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present	Regionally restricted	1Aj	Authors report hindlimb buds have engaged the myogenic program.

1Al ©

Specimen 1Al: embryonic day 11.5; Six1^tm1Mair/Six1^tm1Mair (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Weak	Single cells	1Al	Expression a few posterior cells.

1An ©

Specimen 1An: embryonic day 11.5; Six1^tm1Mair/Six1^tm1Mair (more

)

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present	Regionally restricted	1An

1Ap ©

Specimen 1Ap: embryonic day 11.5; Six1^tm1Mair/Six1^tm1Mair, Six4^tm1Mair/Six4⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Absent		1Ap

1Ar ©

Specimen 1Ar: embryonic day 11.5; Six1^tm1Mair/Six1^tm1Mair, Six4^tm1Mair/Six4⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Weak	Single cells	1Ar	Expression a few posterior cells.

6(I) b ©

Specimen 6(I)b: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present		6(I) b
TS19: hindlimb bud	Present		6(I) b

6(I) d ©

Specimen 6(I)d: embryonic day 11.5; Six1^tm1Mair/Six1^tm1Mair (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present		6(I) d
TS19: hindlimb bud	Present		6(I) d

6(I) f ©

Specimen 6(I)f: embryonic day 11.5; Six1^tm1Mair/Six1^tm1Mair, Six4^tm1Mair/Six4⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Absent		6(I) f
TS19: hindlimb bud	Present		6(I) f

Specimen 6(II) Pax3 PHL wt: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present		6(II) Pax3 PHL wt

Specimen 6(II) Pax3 PHL Six1-/-: embryonic day 11.5; Six1^tm1Mair/Six1^tm1Mair (more

)

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present		6(II) Pax3 PHL Six1-/-

Specimen 6(II) Pax3 PHL Six1-/-Six4-/+: embryonic day 11.5; Six1^tm1Mair/Six1^tm1Mair, Six4^tm1Mair/Six4⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Weak	Regionally restricted	6(II) Pax3 PHL Six1-/-Six4-/+	Weak expressin in ventral aspect.

Specimen 6(II) Pax3 FL wt: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present		6(II) Pax3 FL wt

Specimen 6(II) Pax3 FL Six1-/-: embryonic day 11.5; Six1^tm1Mair/Six1^tm1Mair (more

)

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Weak	Regionally restricted	6(II) Pax3 FL Six1-/-	Weak expressin in ventral aspect.

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