Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:122815
Giordani J, et al., Six proteins regulate the activation of Myf5 expression in embryonic mouse limbs. Proc Natl Acad Sci U S A. 2007 Jul 3;104(27):11310-11315
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:6504945
|
|
Gene symbol:
|
Pax3
|
|
Gene name:
|
paired box 3
|
|
Probe:
|
Pax3 probe54
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
1Aa:
embryonic day 10.5
(more  )
Specimen
1Aa:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
1Aa
|
Expression in myogenic progenitor cells.
|
Specimen
1Ab:
embryonic day 10.5
(more )
Specimen
1Ab:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1Ac:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas
|
|
Mutant Allele(s):
|
Six1tm1Mair/Six1tm1Mair
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Weak |
Regionally restricted |
1Ac
|
Authors report reduced number of myogenic progenitor cells.
|
Specimen
1Ad:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas
|
|
Mutant Allele(s):
|
Six1tm1Mair/Six1tm1Mair
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud |
Weak |
Regionally restricted |
1Ad
|
Reduced number of myogenic progenitor cells entering from hypaxial dermomyotome of adjacent somite
|
| TS17: dermomyotome |
Present |
|
1Ad
|
|
Specimen
1Ae:
embryonic day 10.5
(more )
Specimen
1Ae:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: embryo |
Present |
Regionally restricted |
1Ae
|
|
Specimen
1Af:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas
|
|
Mutant Allele(s):
|
Six1tm1Mair/Six1tm1Mair
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: embryo |
Present |
Regionally restricted |
1Af
|
|
Specimen
1Ah:
embryonic day 11.5
(more )
Specimen
1Ah:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
1Ah
|
Myogenic progenitor cells persent in forelimb buds.
|
Specimen
1Aj:
embryonic day 11.5
(more )
Specimen
1Aj:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindlimb bud |
Present |
Regionally restricted |
1Aj
|
Authors report hindlimb buds have engaged the myogenic program.
|
Specimen
1Al:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas
|
|
Mutant Allele(s):
|
Six1tm1Mair/Six1tm1Mair
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Weak |
Single cells |
1Al
|
Expression a few posterior cells.
|
Specimen
1An:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas
|
|
Mutant Allele(s):
|
Six1tm1Mair/Six1tm1Mair
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1Ap:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas
|
|
Mutant Allele(s):
|
Six1tm1Mair/Six1tm1Mair
Six4tm1Mair/Six4+
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1Ar:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas
|
|
Mutant Allele(s):
|
Six1tm1Mair/Six1tm1Mair
Six4tm1Mair/Six4+
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindlimb bud |
Weak |
Single cells |
1Ar
|
Expression a few posterior cells.
|
Specimen
6(I)b:
embryonic day 11.5
(more )
Specimen
6(I)b:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
6(I)d:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas
|
|
Mutant Allele(s):
|
Six1tm1Mair/Six1tm1Mair
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
6(I)f:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas
|
|
Mutant Allele(s):
|
Six1tm1Mair/Six1tm1Mair
Six4tm1Mair/Six4+
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
6(II) Pax3 PHL wt:
embryonic day 11.5
(more )
Specimen
6(II) Pax3 PHL wt:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
6(II) Pax3 PHL Six1-/-:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas
|
|
Mutant Allele(s):
|
Six1tm1Mair/Six1tm1Mair
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
6(II) Pax3 PHL Six1-/-Six4-/+:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas
|
|
Mutant Allele(s):
|
Six1tm1Mair/Six1tm1Mair
Six4tm1Mair/Six4+
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
6(II) Pax3 FL wt:
embryonic day 11.5
(more )
Specimen
6(II) Pax3 FL wt:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
6(II) Pax3 FL Six1-/-:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas
|
|
Mutant Allele(s):
|
Six1tm1Mair/Six1tm1Mair
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Analysis Tools