Gene Expression Data
Assay Details
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Assay
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Reference:
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J:163986
Bush JO, et al., Ephrin-B1 forward signaling regulates craniofacial morphogenesis by controlling cell proliferation across Eph-ephrin boundaries. Genes Dev. 2010 Sep 15;24(18):2068-80
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6501128
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Gene symbol:
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Ephb3
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Gene name:
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Eph receptor B3
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Results
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Specimen
2B/C:
embryonic day 13.5
(more  )
Note: Double labeled: green - Ephb3; red - Efnb1 (assay MGI:6501127).
Specimen
2B/C:
(close  )
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Genetic Background:
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involves: 129S4/SvJaeSor
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Ephb3; red - Efnb1 (assay MGI:6501127).
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Specimen
2E/F:
embryonic day 13.5; Efnb1tm1.1Sor/Efnb1 +
(more )
Note: Double labeled: green - Ephb3; red - Efnb1 (assay MGI:6501127).
Specimen
2E/F:
(close )
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Genetic Background:
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involves: 129S4/SvJaeSor
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Mutant Allele(s):
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Efnb1tm1.1Sor/Efnb1+
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Age:
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embryonic day 13.5
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Ephb3; red - Efnb1 (assay MGI:6501127).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: palatal shelf mesenchyme |
Present |
Regionally restricted |
2F
,
2E
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Expression upregulated within palatal shelf mesenchyme in domains lacking ephrin-B1 (Efnb1) expression. Mosaic expression in pattern complementary to that of ephrin-B1, with a boundary of one to two cells separating domains.
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Specimen
2H/I:
embryonic day 13.5; Efnb1tm1.1Sor/?
(more )
Note: Ephrin-B1 null mice may be either ephrin-B1-/- or ephrin-B1-/Y. No phenotypic differences have been observed. Double labeled: green - Ephb3; red - Efnb1 (assay MGI:6501127).
Specimen
2H/I:
(close )
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Genetic Background:
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involves: 129S4/SvJaeSor
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Mutant Allele(s):
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Efnb1tm1.1Sor/?
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Ephrin-B1 null mice may be either ephrin-B1-/- or ephrin-B1-/Y. No phenotypic differences have been observed. Double labeled: green - Ephb3; red - Efnb1 (assay MGI:6501127).
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Specimen
3C:
embryonic day 13.5
(more )
Specimen
3C:
(close )
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Genetic Background:
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involves: 129S4/SvJaeSor
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: palatal shelf |
Absent |
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3C
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Ephb3 protein expression not detectable in palatal shelf.
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Specimen
3G:
(close )
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Genetic Background:
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involves: 129S4/SvJaeSor
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Mutant Allele(s):
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Efnb1tm1.1Sor/Efnb1+
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Age:
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embryonic day 13.5
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: palatal shelf |
Present |
Regionally restricted |
3G
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Ephb3 protein up-regulated specifically in ephrin-B1 mutant domains.
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Specimen
3K:
(close )
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Genetic Background:
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involves: 129S4/SvJaeSor
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Mutant Allele(s):
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Efnb1tm1.1Sor/Efnb1+
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Age:
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embryonic day 13.5
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: telencephalon |
Present |
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3K
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No up-regulation of expression complementary to that of ephrin-B1 (Efbn1).
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Specimen
S3B:
embryonic day 13.5; Efnb1tm1.1Sor/?
(more )
Note: Ephrin-B1 null mice may be either ephrin-B1-/- or ephrin-B1-/Y. No phenotypic differences have been observed.
Specimen
S3B:
(close )
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Genetic Background:
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involves: 129S4/SvJaeSor
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Mutant Allele(s):
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Efnb1tm1.1Sor/?
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Ephrin-B1 null mice may be either ephrin-B1-/- or ephrin-B1-/Y. No phenotypic differences have been observed.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: palatal shelf |
Present |
Regionally restricted |
S3B
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Ephb3 protein expression very similar to Ephb3 mRNA expression.
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Specimen
S3F:
embryonic day 13.5
(more )
Note: Genotype indicated as ephrin-B1+/Y.
Specimen
S3F:
(close )
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Genetic Background:
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involves: 129S4/SvJaeSor
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Age:
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embryonic day 13.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Genotype indicated as ephrin-B1+/Y.
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Specimen
S3H:
(close )
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Genetic Background:
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involves: 129S4/SvJaeSor
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Mutant Allele(s):
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Efnb1tm4.2Sor/Efnb1+
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Age:
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embryonic day 13.5
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: palatal shelf |
Present |
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S3H
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Expression not upregulated compared to ephrin-B1+/Y mice.
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Specimen
S3J:
(close )
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Genetic Background:
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involves: 129S4/SvJaeSor
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Mutant Allele(s):
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Efnb1tm4.2Sor/Y
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Age:
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embryonic day 13.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: palatal shelf |
Present |
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S3J
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Expression not upregulated compared to ephrin-B1+/Y mice.
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Analysis Tools