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Gene Expression Data
Assay Details
Assay
Reference: J:163986 Bush JO, et al., Ephrin-B1 forward signaling regulates craniofacial morphogenesis by controlling cell proliferation across Eph-ephrin boundaries. Genes Dev. 2010 Sep 15;24(18):2068-80
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6501127
Gene symbol: Efnb1
Gene name: ephrin B1
Results
Specimen 1D: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: palatal shelf mesenchyme Present Regionally restricted 1D Expression restricted to anterior palatal shelf mesenchyme.
Specimen 1H: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: palatal shelf mesenchyme Present Regionally restricted 1H More weakly expressed in middle positions.
Specimen 1L: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: palatal shelf mesenchyme Present Regionally restricted 1L Expression not detected in posterior palate.
Specimen 1M: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: palatal shelf Absent 1M Expression not significantly detectable in primordial palatal mesenchyme at E11.5.
Specimen 1N: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: palatal shelf mesenchyme Strong Regionally restricted 1N Robust expression within palatal msenchyme by E12.5. Expression first detected in anterior secondary palate, strongest at most distal tip of palatal shelf mesenchyme.
Specimen 1O: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: palatal shelf mesenchyme Strong Widespread 1O Expression remains highly restricted to mesenchyme during outgrowth of palatal shelves. Expression strengthens and expands to encompass most of anterior palatal shelf mesenchyme.
Specimen 1P: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: palatal shelf mesenchyme Strong Regionally restricted 1P Expression remains highly restricted to mesenchyme after elevation of palatal shelves.
Specimen not shown: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: palatal shelf Ambiguous Authors report expression is downregulated around E15.5.
Specimen 2A/C: embryonic day 13.5 (more )
Note: Double labeled: red - Efnb1; green - Ephb3 (assay MGI:6501128).
Structure Level Pattern Image Note
TS21: palatal shelf mesenchyme Present Regionally restricted 2A , 2C Expression in anterior palatal shelves.
TS21: upper jaw mesenchyme Present Regionally restricted 2A , 2C No expression in maxillary mesenchyme lateral to oral cavity.
Specimen 2D/F: embryonic day 13.5; Efnb1tm1.1Sor/Efnb1+ (more )
Note: Double labeled: red - Efnb1; green - Ephb3 (assay MGI:6501128).
Structure Level Pattern Image Note
TS21: palatal shelf mesenchyme Present Regionally restricted 2D , 2F Large mosaic domains of expression in anterior palatal shelves.
Specimen 2G/I: embryonic day 13.5; Efnb1tm1.1Sor/? (more )
Note: Ephrin-B1 null mice may be either ephrin-B1-/- or ephrin-B1-/Y. No phenotypic differences have been observed. Double labeled: red - Efnb1; green - Ephb3 (assay MGI:6501128).
Structure Level Pattern Image Note
TS21: palatal shelf Absent 2G , 2I
Specimen S2A/C: embryonic day 13.5 (more )
Note: Double labeled: red - Efnb1; green - Ephb2 (assay MGI:6501129).
Structure Level Pattern Image Note
TS21: palatal shelf mesenchyme Present Regionally restricted S2C , S2A Expression in anterior palatal shelves.
Specimen S2D/F: embryonic day 13.5; Efnb1tm1.1Sor/Efnb1+ (more )
Note: Double labeled: red - Efnb1; green - Ephb2 (assay MGI:6501129).
Structure Level Pattern Image Note
TS21: palatal shelf mesenchyme Present Regionally restricted S2F , S2D Mosaic domains of expression in anterior palatal shelves.
Specimen S2G/I: embryonic day 13.5; Efnb1tm1.1Sor/? (more )
Note: Ephrin-B1 null mice may be either ephrin-B1-/- or ephrin-B1-/Y. No phenotypic differences have been observed. Double labeled: red - Efnb1; green - Ephb2 (assay MGI:6501129).
Structure Level Pattern Image Note
TS21: palatal shelf Absent S2G , S2I
Specimen 3A: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: palatal shelf mesenchyme Present Widespread 3A Expression throughout palatal shelf mesenchyme.
Specimen 3E: embryonic day 13.5; Efnb1tm1.1Sor/Efnb1+ (more )
Structure Level Pattern Image Note
TS21: palatal shelf Present Regionally restricted 3E Ephrin-B1 (Efnb1) protein expression in mosaic pattern, identical to that of ephrin-B1 mRNA.
Specimen 3I: embryonic day 13.5; Efnb1tm1.1Sor/Efnb1+ (more )
Structure Level Pattern Image Note
TS21: telencephalon Present Regionally restricted 3I Authors report mosaic domains of ephrin-B1 (Efnb1) protein.
Specimen S3A: embryonic day 13.5; Efnb1tm1.1Sor/? (more )
Note: Ephrin-B1 null mice may be either ephrin-B1-/- or ephrin-B1-/Y. No phenotypic differences have been observed.
Structure Level Pattern Image Note
TS21: palatal shelf Absent S3A
Specimen S3E: embryonic day 13.5 (more )
Note: Genotype indicated as ephrin-B1+/Y.
Structure Level Pattern Image Note
TS21: palatal shelf Present S3E
Specimen S3G: embryonic day 13.5; Efnb1tm4.2Sor/Efnb1+ (more )
Structure Level Pattern Image Note
TS21: palatal shelf Present S3G
Specimen S3I: embryonic day 13.5; Efnb1tm4.2Sor/Y (more )
Structure Level Pattern Image Note
TS21: palatal shelf Present S3I
Specimen S5A: embryonic day 13.5 (more )
Note: Triple labeled: green - Efnb1; red - BrdU; blue - DAPI.
Structure Level Pattern Image Note
TS21: telencephalon Present S5A
Specimen S5B: embryonic day 13.5; Efnb1tm1.1Sor/Efnb1+ (more )
Note: Triple labeled: green - Efnb1; red - BrdU; blue - DAPI.
Structure Level Pattern Image Note
TS21: telencephalon Present Regionally restricted S5B Authors report ephrin-B1 (Efnb1) expressing and non-expressing domains.
Specimen S5D: embryonic day 13.5; Efnb1tm1.1Sor/Efnb1+ (more )
Note: Triple labeled: green - Efnb1; red - BrdU; blue - DAPI.
Structure Level Pattern Image Note
TS21: palatal shelf Present Regionally restricted S5D Authors report ephrin-B1 (Efnb1)-negattive cells are under-represented.
Specimen 6F: embryonic day 14.25 (more )
Note: Triple labeled: green - Efnb1; red - BrdU; blue - DAPI.
Structure Level Pattern Image Note
TS22: palatal shelf Present 6F
Specimen 6H: embryonic day 14.25; Efnb1tm1.1Sor/Efnb1+ (more )
Note: Triple labeled: green - Efnb1; red - BrdU; blue - DAPI.
Structure Level Pattern Image Note
TS22: palatal shelf Present Regionally restricted 6H Expression-positive and expression-negative regions.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory