Myh6 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:178848 An CI, et al., Genome-wide mapping of Sox6 binding sites in skeletal muscle reveals both direct and indirect regulation of muscle terminal differentiation by Sox6. BMC Dev Biol. 2011;11:59
Assay type:	RT-PCR
MGI Accession ID:	MGI:6492198
Gene symbol:	Myh6
Gene name:	myosin, heavy polypeptide 6, cardiac muscle, alpha

Probe:	Myh6-pG, Myh6-pH
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR. Relative expression is shown normalized to beta-actin expression. Values are summarized in Table 2.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
Control-1	P m 2	TS28: extensor digitorum longus	Present	Not Specified; total RNA	involves: 129	Sox6^tm2.1Vlf/Sox6^tm2.1Vlf	Not Specified
Control-2	P m 2	TS28: extensor digitorum longus	Present	Not Specified; total RNA	involves: 129	Sox6^tm2.1Vlf/Sox6^tm2.1Vlf	Not Specified
Control-3	P m 3	TS28: extensor digitorum longus	Present	Not Specified; total RNA	involves: 129	Sox6^tm2.1Vlf/Sox6^tm2.1Vlf	Not Specified
KO-1 EDL	P m 2	TS28: extensor digitorum longus	Present (a)	Not Specified; total RNA	involves: 129 * FVB	Sox6^tm2.1Vlf/Sox6^tm2.1Vlf, Tg(Ckmm-cre)5Khn/0	Not Specified	Conditional mutant.
KO-2 EDL	P m 2	TS28: extensor digitorum longus	Present (b)	Not Specified; total RNA	involves: 129 * FVB	Sox6^tm2.1Vlf/Sox6^tm2.1Vlf, Tg(Ckmm-cre)5Khn/0	Not Specified	Conditional mutant.
KO-3 EDL	P m 3	TS28: extensor digitorum longus	Present (c)	Not Specified; total RNA	involves: 129 * FVB	Sox6^tm2.1Vlf/Sox6^tm2.1Vlf, Tg(Ckmm-cre)5Khn/0	Not Specified	Conditional mutant.
-	Other lane(s) in blot assay different gene(s)

Notes:
(a) 24.19 fold change in mRNA levels in the mutant muscle compared to control.
(b) 25.37 fold change in mRNA levels in the mutant muscle compared to control.
(c) 6.65 fold change in mRNA levels in the mutant muscle compared to control.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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