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Gene Expression Data
Assay Details
Assay
Reference: J:84975 Bach A, et al., Msx1 is required for dorsal diencephalon patterning. Development. 2003 Sep;130(17):4025-36
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6489890
Gene symbol: Msx1
Gene name: msh homeobox 1
Reporter: lacZ
Direct Detection
Results
Specimen not shown: embryonic day 12.5; Msx1tm1Bero/? (more )
Structure Level Pattern Image Note
TS20: brain Present Regionally restricted B-gal activity is seen in the ependymal layer at the dorsal midline of the brain over its entire length.
TS20: subcommissural organ primordium Present
Specimen 2A: embryonic day 14.5; Msx1tm1Bero/Msx1tm1Bero (more )
Structure Level Pattern Image Note
TS22: prosomere 1 Absent 2A In 15 of 15 embryos examined there is lack of B-gal activity in the midline of the pretectum. In a few cases a short remnant of the B-gal domain could be detected just posterior to the pineal anlage.
TS22: subcommissural organ Absent 2A
TS22: midbrain Present Regionally restricted 2A B-gal activity is seen at the dorsal midline.
Specimen not shown: embryonic day 14.5; Msx1tm1Bero/Msx1tm1Bero (more )
Structure Level Pattern Image Note
TS22: prosomere 1 Present Regionally restricted In 15 of 15 embryos examined there is lack of B-gal activity in the midline of the pretectum. In a few cases a short remnant of the B-gal domain could be detected just posterior to the pineal anlage.
Specimen 2B: embryonic day 14.5; Msx1tm1Bero/Msx1+ (more )
Structure Level Pattern Image Note
TS22: prosomere 1 Present Regionally restricted 2B B-gal activity is seen at the dorsal midline.
TS22: subcommissural organ Present 2B
TS22: midbrain Present Regionally restricted 2B B-gal activity is seen at the dorsal midline.
Specimen 2C: embryonic day 12.5; Msx1tm1Bero/Msx1tm1Bero (more )
Note: dorsal view
Structure Level Pattern Image Note
TS20: prosomere 1 Absent 2C At E12.5 B-gal activity (or lack thereof) in the mutant varied from a total absence to an apparently normal appearance.
TS20: subcommissural organ primordium Absent 2C
TS20: midbrain Present Regionally restricted 2C B-gal activity is seen at the dorsal midline.
Specimen not shown: embryonic day 12.5; Msx1tm1Bero/Msx1tm1Bero (more )
Structure Level Pattern Image Note
TS20: prosomere 1 Present Regionally restricted At E12.5 B-gal activity (or lack thereof) in the mutant varied from a total absence to an apparently normal appearance.
Specimen 2D: embryonic day 12.5; Msx1tm1Bero/Msx1+ (more )
Note: dorsal view
Structure Level Pattern Image Note
TS20: prosomere 1 Present Regionally restricted 2D B-gal activity is seen at the dorsal midline.
TS20: subcommissural organ primordium Present 2D
TS20: midbrain Present Regionally restricted 2D B-gal activity is seen at the dorsal midline.
Specimen 4C: embryonic day 11.5; Msx1tm1Bero/Msx1tm1Bero (more )
Note: embryo was also stained with Nile Blue to visualize dying cells
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted 4C Although there is variability in the mutants, expression is often seen at the midline at this age.
TS19: midbrain Present Regionally restricted 4C Although there is variability in the mutants, expression is often seen at the midline at this age.
TS19: prosomere 1 Present Regionally restricted 4C Although there is variability in the mutants, expression is often seen at the midline at this age.
Specimen 6A: embryonic day 9.5; Msx1tm1Bero/Msx1+ (more )
Note: Double labeled: blue - Msx1 reporter; purple - Wnt1 (assay MGI:6489888)
Structure Level Pattern Image Note
TS15: diencephalon Present Regionally restricted 6A B-gal activity is seen at the midline.
TS15: future midbrain Present Regionally restricted 6A B-gal activity is seen at the midline.
Specimen 6B: embryonic day 9.5; Msx1tm1Bero/Msx1tm1Bero (more )
Note: Double labeled: blue - Msx1 reporter; purple - Wnt1 (assay MGI:6489888)
Structure Level Pattern Image Note
TS15: diencephalon Present Regionally restricted 6B B-gal activity is seen at the midline in most embryos.
TS15: future midbrain Present Regionally restricted 6B B-gal activity is seen at the midline.
Specimen 6C: embryonic day 10.5; Msx1tm1Bero/Msx1+ (more )
Note: Double labeled: blue - Msx1 reporter; purple - Wnt1 (assay MGI:6489888)
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted 6C B-gal activity is seen at the midline.
TS17: midbrain Present Regionally restricted 6C B-gal activity is seen at the midline.
Specimen 6D: embryonic day 10.5; Msx1tm1Bero/Msx1tm1Bero (more )
Note: Double labeled: blue - Msx1 reporter; purple - Wnt1 (assay MGI:6489888)
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted 6D Although there is more variability at this age, at least some embryos showed B-gal activity at the midline.
TS17: midbrain Present Regionally restricted 6D Expression is seen at the midline.
Specimen 8C: embryonic day 12.5; Msx1tm1Bero/Msx1+ (more )
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 8C B-gal activity is seen at the midline.
TS20: posterior commissure Present Regionally restricted 8C B-gal activity is seen at the midline.
TS20: prosomere 1 Present Regionally restricted 8C B-gal activity is seen at the midline.
TS20: epithalamus Strong Regionally restricted 8C B-gal activity is seen in the pineal gland.
TS20: lateral ventricle choroid plexus Strong 8C
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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