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Gene Expression Data
Assay Details
Assay
Reference: J:84975 Bach A, et al., Msx1 is required for dorsal diencephalon patterning. Development. 2003 Sep;130(17):4025-36
Assay type: RNA in situ
MGI Accession ID: MGI:6489888
Gene symbol: Wnt1
Gene name: wingless-type MMTV integration site family, member 1
Probe: Wnt1 cDNA18
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 3C: embryonic day 11.5 (more )
Note: wild type
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted 3C Expression is seen at the midline.
TS19: midbrain Present Regionally restricted 3C Expression is seen at the midline.
TS19: prosomere 1 Present Regionally restricted 3C Expression is seen at the midline.
Specimen 3D: embryonic day 11.5; Msx1tm1Bero/Msx1tm1Bero (more )
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted 3D Expression is seen at the midline; in the mutant it is interrupted in the pretectum midline.
TS19: midbrain Present Regionally restricted 3D Expression is seen at the midline.
Specimen 6A: embryonic day 9.5; Msx1tm1Bero/Msx1+ (more )
Note: Double labeled: purple - Wnt1; blue - Msx1 reporter (assay MGI:6489890)
Structure Level Pattern Image Note
TS15: diencephalon Present Regionally restricted 6A Expression is seen at the midline.
TS15: midbrain-hindbrain junction Present 6A Expression is seen in the isthmus.
Specimen 6B: embryonic day 9.5; Msx1tm1Bero/Msx1tm1Bero (more )
Note: Double labeled: purple - Wnt1; blue - Msx1 reporter (assay MGI:6489890)
Structure Level Pattern Image Note
TS15: diencephalon Present Regionally restricted 6B Expression is limited to a few scattered cells in prosomere 1.
TS15: midbrain-hindbrain junction Present 6B Expression in the isthmus is unaffected in the mutant.
Specimen 6C: embryonic day 10.5; Msx1tm1Bero/Msx1+ (more )
Note: Double labeled: purple - Wnt1; blue - Msx1 reporter (assay MGI:6489890)
Structure Level Pattern Image Note
TS17: midbrain Present Regionally restricted 6C Expression is seen at the midline.
TS17: diencephalon Present Regionally restricted 6C Expression is seen at the midline of the posterior diencephalon.
Specimen 6D: embryonic day 10.5; Msx1tm1Bero/Msx1tm1Bero (more )
Note: Double labeled: purple - Wnt1; blue - Msx1 reporter (assay MGI:6489890)
Structure Level Pattern Image Note
TS17: midbrain Present Regionally restricted 6D Expression is seen at the midline.
TS17: diencephalon Present Regionally restricted 6D In the mutant expression is limited to a few patches of cells.
Specimen 8A: embryonic day 10.5 (more )
Note: wild type
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted 8A Expression is seen at the midline.
TS17: midbrain Present Regionally restricted 8A Expression is seen at the midline.
Specimen 8B: embryonic day 10.5; Msx1tm1Bero/Msx1tm1Bero, Msx2tm1Bero/Msx2tm1Bero (more )
Structure Level Pattern Image Note
TS17: diencephalon Absent 8B
TS17: midbrain Present Regionally restricted 8B Expression is observed only caudally in the mesencephalon.
Specimen not shown: embryonic day 11.5; Msx1tm1Bero/Msx1tm1Bero, Msx2tm1Bero/Msx2tm1Bero (more )
Structure Level Pattern Image Note
TS19: diencephalon Absent
TS19: midbrain Present Regionally restricted Expression is observed only caudally in the mesencephalon.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory