Probe:	Sox4 cDNA5
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS13: head	Strong		1A

Structure	Level	Pattern	Image	Note
TS13: neural tube	Present	Regionally restricted	1B	Expression is in the posterior end.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Strong		1C
TS17: future spinal cord	Strong		1C
TS17: midbrain	Strong		1C
TS17: limb	Present		1C

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Present		1D
TS17: limb	Present		1D
TS17: future spinal cord	Strong	Regionally restricted	1D	Expression is in two stripes.

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	2B	Expression is in the sub-ventricular zone.

Structure	Level	Pattern	Image	Note
TS19: neural tube lateral wall	Present	Regionally restricted	2E	Expression is in the sub-ventricular zone. Expression is low in the ventricular zone with scattered higher expressing cells.

Structure	Level	Pattern	Image	Note
TS20: spinal cord lateral wall	Strong	Regionally restricted	2H	Expression is in the sub-ventricular zone and is stronger dorsally.
TS20: spinal cord ventricular layer	Weak		2H	Expression is low in the ventricular zone with scattered higher expressing cells.

Structure	Level	Pattern	Image	Note
TS21: dorsal grey horn	Strong		2K

Structure	Level	Pattern	Image	Note
TS24: dorsal grey horn	Present		2N

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Strong	Regionally restricted	3A	Expression is in the primordial plexiform layer.

Structure	Level	Pattern	Image	Note
TS21: main olfactory bulb layer	Strong	Regionally restricted	3C	Expression is in the primordial plexiform layer.

Structure	Level	Pattern	Image	Note
TS24: cortical plate	Strong	Regionally restricted	3E	Expression is in the dorsal cortical plate.
TS24: cerebral cortex subventricular zone	Absent		3E

Structure	Level	Pattern	Image	Note
TS27: cortical layer V	Present		3G
TS27: cortical layer VI	Present		3G
TS27: cortical plate	Present		3G

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Weak	Regionally restricted	3I	Expression is in the cortical plate and is absent in the deeper layer.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	4A	Expression is in the subventricular zone.

Structure	Level	Pattern	Image	Note
TS21: thalamus	Present	Regionally restricted	4C	Expression is in the differentiating region.

Structure	Level	Pattern	Image	Note
TS24: thalamus	Present	Regionally restricted	4E	Expression is in the medial and lateral region of the thalamus.

Structure	Level	Pattern	Image	Note
TS24: hippocampus	Present	Regionally restricted	5A	Expression is in the hippocampal inferior band and strongly in the intermediate zone of the CA1 region.
TS24: dentate gyrus	Present	Regionally restricted	5A	Expression is in a population of cells migrating from the primary dentate neuroepithelium.

Structure	Level	Pattern	Image	Note
TS27: CA1 pyramidal cell layer	Present		5C
TS27: dentate gyrus	Present	Regionally restricted	5C	Expression is in the migrating granule cell precursors.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Present		5E

Structure	Level	Pattern	Image	Note
TS27: cerebellum external granule cell layer	Trace		6A
TS27: cerebellum ventricular layer	Weak		6A
TS27: cerebellum layer	Strong	Regionally restricted	6A	Expression is in a broad region immediately below the external granule layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Present	Regionally restricted	6D , 6C	Expression is strong in the deep layer and at lower levels in the superficial layer.
TS28: cerebellum internal granule cell layer	Weak		6D , 6C
TS28: cerebellum white matter	Present		6C , 6D

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Absent
TS28: spinal cord lateral wall	Absent