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Gene Expression Data
Assay Details
Assay
Reference: J:130649 Fossat N, et al., A new GFP-tagged line reveals unexpected Otx2 protein localization in retinal photoreceptors. BMC Dev Biol. 2007;7:122
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6479807
Gene symbol: Otx2
Gene name: orthodenticle homeobox 2
Reporter: GFP
Direct Detection
Results
Specimen 2b OTX2-GFP +/OG: embryonic day 9.5; Otx2tm3Tlam/Otx2+ (more )
Structure Level Pattern Image Note
TS15: future brain Present 2b Otx2-GFP +/OG
Specimen 2b OTX2-GFP OG/OG weak: embryonic day 9.5; Otx2tm3Tlam/Otx2tm3Tlam (more )
Structure Level Pattern Image Note
TS15: future brain Present 2b Otx2-GFP OG/OG weak Authors report variable reduction in size of brain from E9.5 on.
Specimen 2b OTX2-GFP OG/OG strong: embryonic day 9.5; Otx2tm3Tlam/Otx2tm3Tlam (more )
Structure Level Pattern Image Note
TS15: future brain Present 2b Otx2-GFP OG/OG strong Authors report variable reduction in size of brain from E9.5 on.
Specimen 2d GFP +/OG: postnatal adult; Otx2tm3Tlam/Otx2+ (more )
Structure Level Pattern Image Note
TS28: photoreceptor layer Present 2d GFP +/OG
Specimen 2d GFP OG/OG: postnatal adult; Otx2tm3Tlam/Otx2tm3Tlam (more )
Structure Level Pattern Image Note
TS28: photoreceptor layer Present 2d GFP OG/OG Photoreceptor cells appeared normal.
Specimen 3a GFP E6.5 +/OG: embryonic day 6.5; Otx2tm3Tlam/Otx2+ (more )
Structure Level Pattern Image Note
TS9: embryo Strong 3a GFP E6.5 +/OG Strong expression in embryonic part of embryos.
Specimen 3a GFP E7.5 +/OG: embryonic day 7.5; Otx2tm3Tlam/Otx2+ (more )
Structure Level Pattern Image Note
TS11: embryo Strong Regionally restricted 3a GFP E7.5 +/OG Expression regionalized toward anterior pole.
Specimen 3b GFP / F-Actin: embryonic day 6.5; Otx2tm3Tlam/Otx2+ (more )
Note: Double labeled: green - Otx2 reporter; red - F-Actin.
Structure Level Pattern Image Note
TS8: embryo Present 3b GFP / F-Actin
Specimen 3b GFP / DNA: embryonic day 6.5; Otx2tm3Tlam/Otx2+ (more )
Structure Level Pattern Image Note
TS8: anterior visceral endoderm Present Regionally restricted 3b GFP / DNA (upper) Nuclear expression detected in both layers.
TS8: epiblast Present Regionally restricted 3b GFP / DNA (upper) Nuclear expression detected in both layers.
Specimen 4a: embryonic day 8.5; Otx2tm3Tlam/Otx2+ (more )
Structure Level Pattern Image Note
TS13: future brain Present 4a Authors state that reporter paralleled that of mRNA expression at all stages.
Specimen 4b: embryonic day 9.5; Otx2tm3Tlam/Otx2+ (more )
Structure Level Pattern Image Note
TS15: future brain Present 4b Authors state that reporter paralleled that of mRNA expression at all stages.
Specimen 4c: embryonic day 10.5; Otx2tm3Tlam/Otx2+ (more )
Structure Level Pattern Image Note
TS17: brain Present Regionally restricted 4c
TS17: telencephalon Weak 4c Decreased expression in telencephalic areas.
Specimen 4d: embryonic day 11.5; Otx2tm3Tlam/Otx2+ (more )
Structure Level Pattern Image Note
TS19: brain Present Regionally restricted 4d
TS19: telencephalon Weak 4d Decreased expression in telencephalic areas.
Specimen 4i: embryonic day 12.5; Otx2tm3Tlam/Otx2+ (more )
Structure Level Pattern Image Note
TS20: olfactory epithelium Present 4i
Specimen 4j: embryonic day 12.5; Otx2tm3Tlam/Otx2+ (more )
Structure Level Pattern Image Note
TS20: eye Present 4j
Specimen 4k: embryonic day 12.5; Otx2tm3Tlam/Otx2+ (more )
Structure Level Pattern Image Note
TS20: inner ear Present 4k
Specimen 5a: embryonic day 16.5; Otx2tm3Tlam/Otx2+ (more )
Structure Level Pattern Image Note
TS24: diencephalon Present 5a
TS24: midbrain Present 5a
TS24: olfactory epithelium Present 5a
Specimen 5b: embryonic day 16.5; Otx2tm3Tlam/Otx2+ (more )
Structure Level Pattern Image Note
TS24: olfactory epithelium Present 5b
Specimen 5c: embryonic day 16.5; Otx2tm3Tlam/Otx2+ (more )
Structure Level Pattern Image Note
TS24: midbrain roof plate Present 5c
Specimen 5d: embryonic day 16.5; Otx2tm3Tlam/Otx2+ (more )
Structure Level Pattern Image Note
TS24: choroid plexus Present 5d
Specimen 5e: postnatal day 2; Otx2tm3Tlam/Otx2+ (more )
Structure Level Pattern Image Note
TS27: brain Present Regionally restricted 5e
Specimen 5f: postnatal day 2; Otx2tm3Tlam/Otx2+ (more )
Structure Level Pattern Image Note
TS27: telencephalon Present Diffuse 5f Expression in diffuse group of cells in basal telencephalon.
Specimen 5g: postnatal day 2; Otx2tm3Tlam/Otx2+ (more )
Structure Level Pattern Image Note
TS27: inferior colliculus Present 5g
TS27: superior colliculus Strong Regionally restricted 5g Stronger expression in superficial optic layers than in deep layers.
Specimen 5h: postnatal day 2; Otx2tm3Tlam/Otx2+ (more )
Structure Level Pattern Image Note
TS27: choroid plexus Present 5h
TS27: cerebellum external granule cell layer Strong Graded 5h High posterior to low anterior expression.
Specimen 5i: postnatal adult; Otx2tm3Tlam/Otx2+ (more )
Structure Level Pattern Image Note
TS28: superior colliculus Strong 5i
Specimen 5j: postnatal adult; Otx2tm3Tlam/Otx2+ (more )
Structure Level Pattern Image Note
TS28: choroid plexus Strong 5j
Specimen 5l/m: postnatal day 6; Otx2tm3Tlam/Otx2+ (more )
Note: Double labeled: green - Otx2 reporter (direct detection of GFP); red - Otx2 reporter (detection of GFP by antibody; assay MGI:6479816).
Structure Level Pattern Image Note
TS28: ventral lateral geniculate nucleus Strong Regionally restricted 5l , 5m Authors report the same number of expressing neurons as seen for GFP immunohistochemistry.
Specimen 6a: postnatal adult; Otx2tm3Tlam/Otx2+ (more )
Note: Double labeled: green - Otx2 reporter; red - propidium iodide.
Structure Level Pattern Image Note
TS28: pigmented retinal epithelium Present Regionally restricted 6a Expression throughout nuclear space in retinal pigment epithelial cells.
TS28: photoreceptor layer outer segment Present 6a
TS28: photoreceptor layer inner segment Present Regionally restricted 6a Expression in cells located at outemost part of internal nuclear layer.
Specimen 6c: postnatal adult; Otx2tm3Tlam/Otx2+ (more )
Note: Double labeled: green - Otx2 reporter; red - Lmnb1 (assay MGI:6479828).
Structure Level Pattern Image Note
TS28: pigmented retinal epithelium Present Regionally restricted 6c Strictly nuclear localization of expression.
Specimen 6d: postnatal adult; Otx2tm3Tlam/Otx2+ (more )
Note: Double labeled: green - Otx2 reporter; red - Lmnb1 (assay MGI:6479828).
Structure Level Pattern Image Note
TS28: photoreceptor layer inner segment Present Regionally restricted 6d Strictly nuclear localization of expression in bipolar cells.
Specimen 6e/e'': postnatal adult; Otx2tm3Tlam/Otx2+ (more )
Note: Double labeled: green - Otx2 reporter; red - Lmnb1 (assay MGI:6479828).
Structure Level Pattern Image Note
TS28: photoreceptor layer outer segment Present Regionally restricted 6e'' , 6e' Expression concentrated at periphery of nuclei, facing inner nuclear envelope.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory