Antibody:	anti-Pax2
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted	1A	Expression in ventral telencephalon. Few expressing cells in lateral sagittal sections.
TS17: diencephalon	Present	Regionally restricted	1A	Expression in future eminentia thalami of diencephalon.
TS17: optic cup	Present		1A

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted	1a	Expression in ventral telencephalon. Few expressing cells in lateral sagittal sections.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted	1a'	Expression in ventral telencephalon. Expression more intense and abundant in medial sections.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	1B	Expression in a small population of cells in neuroepithelium of anterior hypothalamus close to optic recess area.
TS17: optic recess	Strong	Regionally restricted	1B	Strong expression in ventral neuroepithelium of optic recess.

Structure	Level	Pattern	Image	Note
TS17: optic recess	Present	Regionally restricted	1b	Strong expression in ventral neuroepithelium of optic recess.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Strong	Regionally restricted	1C	Strong expression in developing septum.
TS19: diencephalon	Present	Regionally restricted	1C	Exprexsion in cells close to base of hypothalamus, and also in more dorsal areas of anterior hypothalamus.
TS19: optic recess	Strong	Regionally restricted	1C	Strong expression in ventral neuroepithelium of optic recess.
TS19: future spinal cord	Present		1C

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Strong	Regionally restricted	1c	Strong expression in developing septum.
TS19: diencephalon	Present	Regionally restricted	1c	Expression in anterior hypothalamus in cells that may originate from ventral neuroepithelium of optic recess area.
TS19: optic recess	Strong		1c	Strong expression in ventral neuroepithelium of optic recess.
TS19: diencephalon lamina terminalis	Absent		1c

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Strong	Regionally restricted	2A	Strong expression in septum of basal forebrain, mainly in septal neuroepithelium.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Strong	Regionally restricted	2a	Expression in most cells of septal neuroepithelium, in close proximity to dorso-medial telencephalon.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	2B	Expression in group of cells in lateral hypothalamic area, and in narrow strip of cells parallel to anterior hypothalamic ventricular zone.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	2b	Expression in group of cells in lateral hypothalamic area, in narrow strip of cells parallel to anterior hypothalamic ventricular zone, and along base of hypothalamus, excluding midline region.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	2b'	Expression in anterior hypothalamic epithelium.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	2C	Expression in small cluster of cells resembling a nucleus in neuroepithelium of anteiorr hypothalamus.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	2c	Expression in groups of cells in lateral hypothalamic area. Expression in anterior hypothalamic epithelium.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Strong	Regionally restricted	2D	Strong expression in septum in region where lamina terminalis joins the septum.
TS20: midbrain-hindbrain junction	Strong		2D	Strong expression in isthmic region.
TS20: spinal cord	Strong		2D

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Strong	Regionally restricted	2d	Strong expression in septum in region where lamina terminalis joins the septum.
TS20: diencephalon lamina terminalis	Present	Single cells	2d	Expression in a few cells in lamina terminalis.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	2d'	Expression in groups of cells in lateral hypothalamic area, in anterior hypothalamic epithelium and in ventral neuroepithelium of optic recess area at base of hypothalamus.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	3B	Expression in septum. Only a few of expressing cells co-express Nes.

Structure	Level	Pattern	Image	Note
TS20: retina	Strong

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Strong	Regionally restricted	2E	Strong expression in cells at septal midline.

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Strong	Regionally restricted	2F	Strong expression in septal neuroepithelium at point where septum joins future hippocampus vias the lamina terminalis.

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	2G	At more rostral levels, few expressing cells in differentiating layer of septum.

Structure	Level	Pattern	Image	Note
TS21: hypothalamus	Present	Regionally restricted		Authors report expression was similar to E12.5, with the exception that expression in lateral hypothalamic area and base of hypothalamus was no longer detectable.

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present	Regionally restricted		Authors report expression in coronal telencephalic sections similar to E13.5.

Structure	Level	Pattern	Image	Note
TS22: telencephalon septum	Strong	Regionally restricted	4A	Strong expression in neuroepithelium of septum adjacent to foramen of Monro.

Structure	Level	Pattern	Image	Note
TS22: telencephalon septum	Strong	Regionally restricted	4a	Strong expression at level where lamina terminalis joins the septum.

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present	Regionally restricted	4B	Expression in narrow band of cells in differentiating field of anterior hypothalamus, reaching medial horn of lateral ventricle.

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present	Regionally restricted	4b	Expression in narrow band of cells in differentiating field of anterior hypothalamus, reaching medial horn of lateral ventricle.

Structure	Level	Pattern	Image	Note
TS24: optic chiasma	Present		4C
TS24: optic stalk	Present	Regionally restricted	4C	Expression in optic stalk epithelium.
TS24: hypothalamus	Present	Regionally restricted	4C	Expression in a few cells of hypothalamic neuroepithelium next to suprachiasmatic nucleus.

Structure	Level	Pattern	Image	Note
TS24: telencephalon septum	Present	Regionally restricted	4D	Expression in differentiating layer of medial septum surrounded by axonal bundles of fornix.

Structure	Level	Pattern	Image	Note
TS27: telencephalon septum	Present	Scattered	5A	Expression in cells scattered in the most caudal sections, at levels where fornix is in proximity fo anterior commissure.

Structure	Level	Pattern	Image	Note
TS27: telencephalon septum	Present	Single cells	5B	Expression in a few cells close to fornix, in medial septal area, and in border zone bewteen medial and lateral septum.
TS27: medial preoptic nucleus	Present		5B

Structure	Level	Pattern	Image	Note
TS28: telencephalon septum	Present	Scattered	5C	Expression in a few dispersed cells in the septal area.

Structure	Level	Pattern	Image	Note
TS28: telencephalon septum	Present	Single cells	5D	Expression in a few dispersed cells in the septal area.

Structure	Level	Pattern	Image	Note
TS28: subfornical organ	Present		5E

Structure	Level	Pattern	Image	Note
TS28: forebrain	Absent			Expression no longer detected at P15.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	6B	Expression in septum.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	6C	Intensity of expression appears reduced and expression domain is shifted dorsally compared to wild type.

Structure	Level	Pattern	Image	Note
TS28: telencephalon septum	Present	Regionally restricted	7A , 7C	Expression in septal area. No co-expression with calbindin (Calb1).

Structure	Level	Pattern	Image	Note
TS27: telencephalon septum	Present	Regionally restricted		No co-expression with calbindin (Calb1) or Islet1 (Isl1).