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Gene Expression Data
Assay Details
Assay
Reference: J:294964 Vincentz JW, et al., Variation in a Left Ventricle-Specific Hand1 Enhancer Impairs GATA Transcription Factor Binding and Disrupts Conduction System Development and Function. Circ Res. 2019 Aug 30;125(6):575-589
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6478001
Gene symbol: Hand1
Gene name: heart and neural crest derivatives expressed 1
Reporter: lacZ
Direct Detection
Results
Specimen 1B: embryonic day 10.5; Hand1tm1Eno/Hand1+ (more )
Structure Level Pattern Image Note
TS17: primitive ventricle Present 1B
Specimen 1E: embryonic day 10.5; Hand1tm1Eno/Hand1+ (more )
Structure Level Pattern Image Note
TS17: primitive ventricle cardiac muscle Present 1E Expression is seen in the left ventricle.
TS17: outflow tract Present 1E Expression is seen in the myocardial cuff of the outflow tract.
TS17: mesenchyme derived from neural crest Present 1E Expression is seen in neural crest cells.
Specimen 4A: postnatal day 42; Hand1tm1Eno/Hand1+ (more )
Structure Level Pattern Image Note
TS28: heart left ventricle Present 4A Expression is seen in the left ventricle septal wall.
TS28: interventricular septum Present 4A Expression is seen in the left ventricle septal wall.
Specimen 4B: postnatal day 42; Rr132em1Abfi/Rr132+, Hand1tm1Eno/Hand1tm1Eno (more )
Structure Level Pattern Image Note
TS28: heart left ventricle Present 4B Expression is seen in the left ventricle septal wall; this staining is increased in this mutant.
TS28: interventricular septum Present 4B Expression is seen in the left ventricle septal wall; this staining is increased in this mutant.
Specimen 4C: postnatal day 7; Hand1tm1Eno/Hand1+ (more )
Note: Double labeled: blue - Hand1 reporter; brown - Cntn2 (assay MGI:6478019)
Structure Level Pattern Image Note
TS28: impulse conducting system Present 4C Expression is seen in the His bundle and left bundle branch.
Specimen 4D: postnatal day 7; Rr132em1Abfi/Rr132+, Hand1tm1Eno/Hand1tm1Eno (more )
Note: Double labeled: blue - Hand1 reporter; brown - Cntn2 (assay MGI:6478019)
Structure Level Pattern Image Note
TS28: impulse conducting system Present 4D Expression is seen in the His bundle and left bundle branch.
Specimen Online 5B: postnatal day 42; Hand1tm1Eno/Hand1+ (more )
Structure Level Pattern Image Note
TS28: heart right ventricle Present Regionally restricted Online 5B' , Online 5B Staining is seen in the base of the right ventricle septum.
TS28: interventricular septum Present Regionally restricted Online 5B' , Online 5B Staining is seen in the base of the right ventricle septum.
Specimen Online 5C: postnatal day 42; Hand1tm1Eno/Hand1+ (more )
Structure Level Pattern Image Note
TS28: heart left ventricle Present Regionally restricted Online 5C , Online 5C' Staining is seen in the wall of the left ventricle septum.
TS28: interventricular septum Present Regionally restricted Online 5C , Online 5C' Staining is seen in the wall of the left ventricle septum.
Specimen Online 5D: postnatal day 42; Hand1tm1Eno/Hand1+ (more )
Structure Level Pattern Image Note
TS28: heart ventricle papillary muscle Present Online 5D Staining is seen surrounding the papillary muscle connecting with the free wall of the left ventricle.
Specimen Online 5E: postnatal day 42; Hand1tm1Eno/Hand1+ (more )
Note: Double labeled: blue - Hand1 reporter; brown - Gja5 (assay MGI:6478283)
Structure Level Pattern Image Note
TS28: impulse conducting system Present Online 5E , Online 5E' Expression is seen in the ventricular conduction system.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory